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Utilization of Dark Moth Wing Gene in Silkworm Genetic Breeding

A technology of genetic breeding and dark moth, applied in the direction of animal husbandry and other directions, can solve the problems of reducing economic traits, the degree of practical application of genetic resources of pollution and darkening, achieving no pollution, avoiding inconsistency of hybridization rate indicators, and simple process Effect

Active Publication Date: 2018-03-27
湖南省蚕桑科学研究所
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  • Abstract
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Problems solved by technology

[0006] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a method to solve the technical problems of pollution caused by artificial dyeing technology and the low degree of practical application of darkening genetic resources. Dark moth wing gene resources, through a method of quickly introducing homozygous dominant genes into a variety in the hybrid combination, so that it can be used without reducing the practicality of economic traits. The significant difference in wing body color between another pair of hybrids in the hybrid combination achieves the purpose of distinguishing and eliminating pure pairs, thereby increasing the hybridization rate of the first generation of hybrids

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  • Utilization of Dark Moth Wing Gene in Silkworm Genetic Breeding

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Embodiment Construction

[0022] Such as figure 1 Shown is that the present invention provides the utilization method of dark moth wing gene in silkworm genetic breeding, comprises the following steps:

[0023] (1) select the silkworm kind that needs transformation as parent;

[0024] (2) Use the basic variety containing the dark moth wing gene to cross with the silkworm variety that needs to be transformed to obtain the heterozygous type F 1 , using F 1 Or the dominant heterozygote obtained in each generation of backcrossing is carried out continuous backcrossing with the silkworm variety selected for transformation to obtain backcrossing breeding materials, and then the backcrossing breeding materials are self-crossed to obtain F 2 , from F 2 F 3 . Since the dark moth wing gene is a dominant gene, and the homozygous individual of the dominant gene has the darkest color, the color of the non-homozygous individual (heterozygous individual) of the dominant gene is lighter than that of the homozygou...

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Abstract

The invention discloses a dark moth wing gene utilization method in silkworm genetic breeding. Through a homozygosis method of rapidly introducing a dominant gene, the dominant gene is introduced into one breed of hybridized combination, so that on the basis that the degree of functionization of economic characters is not lowered, and by utilizing the differences of obvious moth wing body color between the dominant gene and another pair of hybridized breed of the hybridized combination, the purposes that pure pairs are distinguished and removed so that the hybrid rate of first generation hybrid cultivar can be improved are achieved. According to the homozygosis method of rapidly introducing the dominant gene, by utilizing the inherent characteristics of a homozygous individual of the dominant gene, individual transgenerational of the dominant gene is selected, the complete homozygous process of the dominant gene can be accelerated, and compared with a traditional breeding method, the number of selecting generations can be substantially reduced. According to the method, the number of generations needed by the gene homozygosis is small, the working procedure is simple, the cost is low, no pollution occurs, the economic characters are good, the practical value is high, by utilizing the preparation characteristics of the dark moth wing gene of the hybridized combination, the hybrid rate of the first generation hybrid cultivar can be substantially improved, and the occurrence that the hybrid rate indicator is not qualified is avoided.

Description

technical field [0001] The invention relates to a silkworm breeding method, in particular to a method for utilizing dark moth wing genes in silkworm genetic breeding. Background technique [0002] According to the regulations of the national silkworm first-generation hybrid inspection standard (NYT 327-1997), the hybridization rate of the first-generation hybrid must not be lower than 95%. As a result, a large number of pure pair matings occurred due to incomplete separation of males and females, and the hybridization rate index was difficult to reach the standard, resulting in the scrapping of whole batches of silkworm eggs. [0003] In the prior art, there are 2 technical methods for avoiding pure pairs in silkworm breeding and production: [0004] 1. Some silkworm egg production units use dyes to dye one of the hybrid varieties of silkworm moths to achieve the purpose of distinguishing pure pairs. Dyestuffs cause silkworm moths to be unable to continue to be used in dee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 艾均文司马杨虎薛宏何行健李学升刘昌文郑颖
Owner 湖南省蚕桑科学研究所
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