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Method for extracting and preparing blood-sugar-reducing substance--secoiridoid glucoside compounds from seeds of Fraxinus rhynchophylla Hance

A technology for reducing blood sugar of the large-leaf white wax tree, which is applied in the field of extraction and preparation of blood sugar lowering substances, can solve the problems of less research on the chemical components of the white wax tree seeds, and achieve the effect of high extraction rate

Inactive Publication Date: 2015-07-08
NORTHWEST UNIV(CN)
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Problems solved by technology

There are a lot of research reports on the chemical constituents of Ash tree at home and abroad, but there are few studies on the chemical constituents of Ash tree seeds

Method used

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  • Method for extracting and preparing blood-sugar-reducing substance--secoiridoid glucoside compounds from seeds of Fraxinus rhynchophylla Hance
  • Method for extracting and preparing blood-sugar-reducing substance--secoiridoid glucoside compounds from seeds of Fraxinus rhynchophylla Hance
  • Method for extracting and preparing blood-sugar-reducing substance--secoiridoid glucoside compounds from seeds of Fraxinus rhynchophylla Hance

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Embodiment Construction

[0032] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0033] 1. Extraction and separation

[0034] see figure 1 Shown, it is the flow chart of the extracting process of the ash tree seeds of the present invention; the ash tree seeds selected in the present invention are produced in Baoji, Shaanxi, and the extraction process of the ash tree seeds is:

[0035] In step a, after crushing the seeds of Ashwagandha japonica, extract them by percolation twice with 75% ethanol, and soak for three days each time to obtain a 75% alcohol extraction solution;

[0036] Step b, taking the extract and performing HPLC detection after micro-membrane filtration;

[0037] From the analysis of HPLC, it was found that the seeds of A. grandiflora mainly contained iridoid glycosides Nuzhenide, GI3, GI5, oleoside dimethyl ester and ligstroside.

[0038] In the above step b, in the...

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Abstract

The invention relates to a method for extracting and preparing a blood-sugar-reducing substance from the seeds of Fraxinus rhynchophylla Hance. The method comprises the following concrete steps: crushing the seeds of Fraxinus rhynchophylla Hance and subjecting the crushed seeds to percolation and extraction with 75% ethanol twice so as to obtain a 75% ethanol extraction solution, wherein the seeds are soaked for three days in each percolation and extraction; carrying out micro-membrane filtration on the 75% ethanol extraction solution and then carrying out HPLC detection; and concentrating 75% ethanol extraction liquid so as to obtain extract, dispersing the extract with water, carrying out extraction with petroleum ether, concentrating a petroleum ether layer solution so as to obtain another extract, laying the extract aside, extracting a water layer with ethyl acetate, concentrating an ethyl acetate layer so as to obtain another extract, carrying out petroleum ether-acetone gradient elution, wherein TLC detection and analysis are cooperatively carried out in the process of elution, flushing a column with ethanol, combining eluate of a same part and carrying out respective drying by distillation so as to obtain separated compounds, wherein the separated compounds include secoiridoid glucoside compounds. The method provided by the invention has high extraction rates of components in the seeds of Fraxinus rhynchophylla Hance and can effectively extract the secoiridoid glucoside compounds capable of reducing blood sugar.

Description

technical field [0001] The invention relates to the field of extraction and preparation of hypoglycemic substances, in particular to a method for extracting and preparing hypoglycemic substances from seeds of ash tree species. Background technique [0002] Fraxinus rhynchophylla Hance, also known as Fraxinus rhynchophylla Hance, is also known as Fraxinus rhynchophylla Hance, which belongs to the genus Fraxinus rhynchophylla Hance. The big-leaf ash tree is a large deciduous tree, 12-15 meters high, the bark is gray-brown or dark gray, the young branches are gray-green, smooth, and lobed when old, the flowering period is April-May, and the fruiting period is September-October. The wood of the big-leaf ash tree is tough, strong and durable, and it is an important material for agricultural tools. The branches are used as weaving materials. It is a good tree species for embankment, sand fixation, and urban greening. Its skin is included in "Chinese Pharmacopoeia" as Qinpi, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63A61P3/10A61K131/00
Inventor 白乃生刘庆超张云婷薛伟明白璐张丽郭甜甜温慧云郭森
Owner NORTHWEST UNIV(CN)
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