Method for increasing amount of spores

A spore quantity and spore technology, which is applied in the field of microbial fermentation engineering, can solve the problems of expanded culture and low cost, and achieve the effects of increasing spore production, less workload, and solving the problem of expanding culture.

Inactive Publication Date: 2015-07-22
FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The embodiment of the present invention provides a method for increasing the amount of spores, which can solve the problem of expanded cultivation in the industrial application of strains with low spore production, and has low cost

Method used

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  • Method for increasing amount of spores

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Experimental program
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Embodiment 1

[0040]Under the optimal culture conditions, the sporulation yield of the high sporulation-yielding Aspergillus oryzae culture is 11.5-12 billion / g dry basis, and the sporulation yield of the culture of the low sporulation-yielding Aspergillus oryzae strain is The amount is 3.5-4 billion / g dry basis. Dry the culture of high-spore-producing strains to a certain amount of moisture, and set aside; using bran, bran, and flour as the main medium raw materials, adding 10% of the culture of high-spore-producing strains of bran weight to replace the bran of the corresponding weight, moistening After mixing the water and materials evenly, sterilize at 121°C, 0.11MPa high-temperature steam for 30 minutes, then cool to room temperature, inoculate the low sporulation-producing Aspergillus oryzae strain and cultivate for 72 hours.

[0041] High sporulation-yielding strain cultures with different bran weight ratios were added to the culture medium, and the spore number and germination rate o...

Embodiment 2

[0045] Under the optimal culture conditions, the sporulation yield of the high-sporulation-yielding Aspergillus oryzae strain culture is 9 billion to 10 billion / g dry basis, and the sporulation yield of the culture of the low sporulation-yielding Aspergillus oryzae strain is The amount is 3.5-4 billion / g dry basis. After the spores were removed from the culture of high sporulation-producing strains, the remaining culture was collected, dried to a certain amount of water, and set aside; rice bran, bran, and flour were used as the main raw materials, and cultures with 10% of the weight of bran were added to replace the corresponding weight After the bran was moistened and evenly mixed, it was sterilized by high-temperature steam at 121°C and 0.11 MPa for 30 minutes, then cooled to room temperature, and inoculated with a low sporulation-producing Aspergillus oryzae strain for 72 hours.

[0046] High sporulation-yielding strain cultures with different bran weight ratios were added...

Embodiment 3

[0050] Under the optimal conditions, the sporulation of the culture of the high sporulation-producing Aspergillus oryzae strain is 11.5-12 billion / g dry basis, and the sporulation of the culture of the low sporulation-producing Aspergillus sojae without adding sporulation accelerator It is 2.5-3 billion / g dry basis. After the spores were removed from the culture of high sporulation-producing strains, the remaining culture was collected, dried to a certain amount of water, and set aside; rice bran, bran, and flour were used as the main raw materials, and cultures with 10% of the weight of bran were added to replace the corresponding weight After the bran was moistened and evenly mixed, it was sterilized by high-temperature steam at 121°C and 0.11MPa for 30 minutes, cooled to room temperature, and inoculated with a low sporulation-producing Aspergillus sojae strain for 72 hours.

[0051] High sporulation-yielding strain cultures with different bran weight ratios were added to th...

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Abstract

The invention discloses a method for increasing the amount of spores. The method comprises the following steps: 1) obtaining high-spore bacterial strain culture with a spore-producing accelerant; 2) performing inactivation treatment on the obtained high-spore bacterial strain culture with the spore-producing accelerant; and 3) adding the high-spore bacterial strain culture with the spore-producing accelerant subjected to the inactivation treatment in the step 2) into a culture medium of a low-spore bacterial strain. Compared with the prior art, the method can solve the enlarged cultivation problem of the low-spore bacterial strain in industrial application, and is low in cost.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for increasing the amount of spores in the process of microbial cultivation. Background technique [0002] As one of the most important industrial microbial strains in fermentation engineering, Aspergillus oryzae is widely used in food fermentation, enzyme engineering and other fields. The quality of strains is the core technology in the microbial industry. Excellent industrial strains, in addition to having excellent metabolic capacity, must also be easy to expand and cultivate. Therefore, in the industrial application of Aspergillus oryzae strains, the formation of spores plays a very important role in product quality, and is a key index for the expanded cultivation of Aspergillus oryzae. The direct purpose of expanding the culture is to obtain a large number of good spores. [0003] If a bacterial strain with excellent traits is obtained through scr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/69
Inventor 周其洋
Owner FOSHAN HAITIAN FLAVOURING & FOOD CO LTD
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