Application of cinnamic acid on preparation of medicines for treating cancers

Abstract

The invention discloses an application of cinnamic acid on preparation of medicines for treating cancers. The cancers comprise a prostatic cancer, a non-Hodgkins lymphoma, spongioblastoma, oophoroma and a colorectal cancer. The cinnamic acid can effectively promote apoptosis of malignant cells, adjusts a proliferation cycle of the malignant cells and restrains proliferation of the malignant cells to a certain degree; and moreover, the cinnamic acid can act on an STAT3 signal channel, obviously reduces expression of STAT3 genes and effectively restrains growth of malignant tumors of the prostatic cancer, the non-Hodgkins lymphoma, the spongioblastoma, the oophoroma and the colorectal cancer in bodies of mice. By the application of the cinnamic acid on preparation of the medicines for treating the cancers, novel medicines with antitumor activity can be conveniently researched on the aspects of cell apoptosis and tissue cell proliferation cycle, prevention and treatment on various malignant tumors which are relevant to STAT3 are researched conveniently, and a novel thinking is provided for treatment of the various malignant tumors.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the use of cinnamic acid in the preparation of medicines for treating prostate cancer, non-Hodgkin's lymphoma, glioblastoma, ovarian cancer, prostate cancer or colorectal cancer. Background technique

[0002] Cinnamic acid, also known as β-phenylacrylic acid or 3-phenyl-2-acrylic acid. It is an organic acid isolated from cinnamon bark or benzoin. It is mainly used in flavors and fragrances, food additives, pharmaceutical industry, beauty, pesticides, organic synthesis, etc.

[0003] Malignant tumors, as one of the largest public health problems in the world, have greatly endangered human health, and have gradually become the number one killer of human beings in today's era. The occurrence and progress of different malignant tumors are very different, but they also have a common feature, that is, the occurrence and development of tumors are largely not only th...

Examples

Embodiment 1

[0027] Example 1: Effect of cinnamic acid on apoptosis of malignant tumor cells

[0028] 1. Experimental materials and grouping

[0029] 1.1 Experimental cells

[0030] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, malignant tumor cells of ovarian cancer), human colorectal cancer cells (HCCT-116 cells, malignant tumor cells of colorectal cancer) and non-Hodgkin's lymphoma cells (Raji cells, non-Hodgkin Malignant tumor cells of gold lymphoma) were routinely cultured according to the characteristics of various cells.

[0031] 1.2 Experimental drugs and equipment

[0032] Cinnamic acid (Aladdin), Annexin-FITC / PI double staining kit (Beyond), high-speed centrifuge, flow cytometer, etc.

[0033] 1.3 Experimental grouping and steps

[0034] For each malignant tumor cell, three groups were set up, respectively name...

Embodiment 2

[0048] Example 2: Effects of cinnamic acid on the proliferation cycle of malignant tumor cells

[0049] 1. Experimental materials and grouping

[0050] 1.1 Experimental cells

[0051] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, malignant tumor cells of ovarian cancer), human colorectal cancer cells (HCCT-116 cells, malignant tumor cells of colorectal cancer) and non-Hodgkin's lymphoma cells (Raji cells, non-Hodgkin Malignant tumor cells of gold lymphoma) were routinely cultured according to the characteristics of various cells.

[0052] 1.2 Experimental drugs and equipment

[0053] Cinnamic acid (Aladdin), PI dye (Beiyuntian), 70% ethanol, high-speed centrifuge, flow cytometer, etc.

[0054] 1.3 Experimental grouping and steps

[0055] For each malignant tumor cell, three groups were set up, respectively n...

Embodiment 3

[0068] Example 3: Effect of cinnamic acid on STAT3 signaling pathway

[0069] 1. Experimental materials

[0070] 1.1 Experimental materials

[0071] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, that is, malignant tumor cells of ovarian cancer), human-derived colorectal cancer cells (HCCT-116 cells, that is, malignant tumor cells of colorectal cancer), were routinely cultured according to the characteristics of various cells.

[0072] 1.2 Experimental drugs and equipment

[0073] Cinnamic acid (Aladdin), AG490 (Gene operation), mRNA extraction kit (Tiangen), cDNA first-strand synthesis kit (Tiangen), PCR instrument, etc. Among them, in this example, α-cyano-(3,4-hydroxy)N-benzyl styrylamine (AG490) was used as a positive control drug to investigate the effect of cinnamic acid on the STAT3 signaling pathway. A...

technical field

[0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the use of cinnamic acid in the preparation of medicines for treating prostate cancer, non-Hodgkin's lymphoma, glioblastoma, ovarian cancer, prostate cancer or colorectal cancer. Background technique

[0002] Cinnamic acid, also known as β-phenylacrylic acid or 3-phenyl-2-acrylic acid. It is an organic acid isolated from cinnamon bark or benzoin. It is mainly used in flavors and fragrances, food additives, pharmaceutical industry, beauty, pesticides, organic synthesis, etc.

[0003] Malignant tumors, as one of the largest public health problems in the world, have greatly endangered human health, and have gradually become the number one killer of human beings in today's era. The occurrence and progress of different malignant tumors are very different, but they also have a common feature, that is, the occurrence and development of tumors are largely not only th...

Examples

Embodiment 1

[0027] Example 1: Effect of cinnamic acid on apoptosis of malignant tumor cells

[0028] 1. Experimental materials and grouping

[0029] 1.1 Experimental cells

[0030] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, malignant tumor cells of ovarian cancer), human colorectal cancer cells (HCCT-116 cells, malignant tumor cells of colorectal cancer) and non-Hodgkin's lymphoma cells (Raji cells, non-Hodgkin Malignant tumor cells of gold lymphoma) were routinely cultured according to the characteristics of various cells.

[0031] 1.2 Experimental drugs and equipment

[0032] Cinnamic acid (Aladdin), Annexin-FITC / PI double staining kit (Beyond), high-speed centrifuge, flow cytometer, etc.

[0033] 1.3 Experimental grouping and steps

[0034] For each malignant tumor cell, three groups were set up, respectively name...

Embodiment 2

[0048] Example 2: Effects of cinnamic acid on the proliferation cycle of malignant tumor cells

[0049] 1. Experimental materials and grouping

[0050] 1.1 Experimental cells

[0051] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, malignant tumor cells of ovarian cancer), human colorectal cancer cells (HCCT-116 cells, malignant tumor cells of colorectal cancer) and non-Hodgkin's lymphoma cells (Raji cells, non-Hodgkin Malignant tumor cells of gold lymphoma) were routinely cultured according to the characteristics of various cells.

[0052] 1.2 Experimental drugs and equipment

[0053] Cinnamic acid (Aladdin), PI dye (Beiyuntian), 70% ethanol, high-speed centrifuge, flow cytometer, etc.

[0054] 1.3 Experimental grouping and steps

[0055] For each malignant tumor cell, three groups were set up, respectively n...

Embodiment 3

[0068] Example 3: Effect of cinnamic acid on STAT3 signaling pathway

[0069] 1. Experimental materials

[0070] 1.1 Experimental materials

[0071] Human malignant glioblastocytes (U87MG cells, malignant tumor cells of glioblastoma), human prostate cancer cells (PC-3 cells, malignant tumor cells of prostate cancer), human ovarian cancer cells (SKOV -3 cells, that is, malignant tumor cells of ovarian cancer), human-derived colorectal cancer cells (HCCT-116 cells, that is, malignant tumor cells of colorectal cancer), were routinely cultured according to the characteristics of various cells.

[0072] 1.2 Experimental drugs and equipment

[0073] Cinnamic acid (Aladdin), AG490 (Gene operation), mRNA extraction kit (Tiangen), cDNA first-strand synthesis kit (Tiangen), PCR instrument, etc. Among them, in this example, α-cyano-(3,4-hydroxy)N-benzyl styrylamine (AG490) was used as a positive control drug to investigate the effect of cinnamic acid on the STAT3 signaling pathway. A...

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