Method, apparatus, and system for detecting fetus gene information

A gene and fetal technology, applied in the field of biological detection, can solve the problems of difficulty in detecting fetal genomic abnormalities, restricting the scope of application, poor robustness, etc. and precision effects

Active Publication Date: 2015-08-12
CHI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Considering the high cost of sequencing, the poor robustness restricts the scope of its application and hinders the further development of this technology, making it difficult to detect more refined fetal genomic abnormalities

Method used

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  • Method, apparatus, and system for detecting fetus gene information
  • Method, apparatus, and system for detecting fetus gene information
  • Method, apparatus, and system for detecting fetus gene information

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Detection of Fetal Chromosomal Aneuploidy

[0062] 1. Blood sample collection and processing

[0063] (1) Pregnant women are 17 weeks pregnant, and 2ml of peripheral blood is collected by vein.

[0064] (2) Separation of blood cells and plasma: place the anticoagulated peripheral blood at 4°C for 30 minutes until the blood sample has obvious stratification, carefully suck out the upper layer of plasma, centrifuge at 4000rpm for 5 minutes, and separate the supernatant maternal plasma from the blood cell pellet.

[0065] (3) Genomic DNA of blood cells was extracted using Tiangen blood DNA group extraction kit (DP318).

[0066] (4) The cfDNA in the plasma was extracted using Magen Fetal DNA Mini-Extraction Kit (D3184).

[0067] (5) Use a Nanodrop 2000 spectrophotometer to measure OD260nm and OD280nm, confirm its high purity, and measure its concentration.

[0068] 2. Large-scale sequencing

[0069] Use the NEBNext Fast DNA Fragmentation&Library Prep Set for Io...

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Abstract

The invention discloses a method for extracting fetus gene information. In the provided method, maternal cell DNA and acellular DNA are separated from a maternal sample, wherein the acellular DNA is from the fetus. Two DNA samples are strictly processed by same reagents and methods and subjected to parallel sequencing in a same sequencing reaction; and the fetus gene information and fetus gene abnormity can be obtained by comparing the sequencing results of two DNA samples. The provided autologous comparing method can be applied to detection of chromosome aneuploidy, and can also be applied to gene detections such as chimera detection, copy number variation detection, single point mutation detection, and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to the detection of fetal gene information. Background technique [0002] The extraction of fetal genetic information is the main source of information for prenatal genetic diagnosis. This method can be used for prenatal diagnosis of genetic diseases suffered by the fetus, such as trisomy 21 (Down syndrome), trisomy 18, trisomy 13, etc. , or determine the mutation of a specific gene, in order to achieve the purpose of prenatal and postnatal care. Compared with protein detection, ultrasound imaging detection and other methods, genetic diagnosis has the characteristics of accuracy and early detection, so it has attracted much attention. Traditional prenatal genetic diagnosis requires amniocentesis sampling, which is an invasive sampling of the uterus. Although it can be performed under ultrasound guidance, it will still increase the risk of miscarriage by 0.5% to 1.5%. Non-invasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G06F19/22
CPCC12Q1/6869G16B30/00C12Q2535/122
Inventor 张弓董鸣
Owner CHI BIOTECH CO LTD
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