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High-strength biomimetic collagen membrane and preparation method thereof

A biomimetic rubber, high-strength technology, used in medical science, prosthesis, surgery, etc., can solve the problems of long production process, reduce medical costs, and high production costs, achieve high mechanical properties, easy mass production, and production costs. low effect

Active Publication Date: 2018-04-06
BEIJING PAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] For the existing technology of using collagen as raw material to make collagen film, the problems to be solved urgently are: using type I collagen as raw material, mainly through the collagen film produced by traditional processes such as dissolving, adding chondroitin sulfate, and freeze-drying , need to add chondroitin sulfate to increase the strength of the material, adding non-degradable substances to the material will increase the risk of bioincompatibility, and the production process is long and the production cost is high, which is not conducive to reducing medical costs

Method used

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  • High-strength biomimetic collagen membrane and preparation method thereof
  • High-strength biomimetic collagen membrane and preparation method thereof
  • High-strength biomimetic collagen membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Step 1, take the pigskin, remove the hair, add delipase and rinse in purified water for 24 hours to remove the fat;

[0059] Step 2, after slicing, add acetic acid solution with a pH value of 1.0, then add pepsin, and enzymatically hydrolyze at 4°C for 90 hours;

[0060] Step 3, after enzymolysis, use a centrifuge to centrifuge the enzymolysis solution to take the supernatant, use an alkaline solution to adjust the pH value to 7, and then use a sodium chloride salt solution to salt out the collagen;

[0061] Step 4: Take the collagen, put it into a dialysis bag, and use the method of gradient dialysis to dissolve it in acetic acid solution for dialysis, including the following steps;

[0062] S1-1: Put 20g of collagen into a dialysis bag with a diameter of 6cm and a length of 50cm, and seal the port of the dialysis bag;

[0063] S1-2: The first gradient dialysis, using pH=2 acetic acid solution for 7 days, the temperature is 4°C, and the dialysate is changed twice a da...

Embodiment 2

[0069] Step 1, take the beef Achilles tendon, add methyl chloride, soak for 24 hours, and remove the fat;

[0070] Step 2, after slicing, add a citric acid solution with a pH value of 2.0, add papain, and enzymolyze at 10°C for 36 hours;

[0071] Step 3, after enzymolysis, use a centrifuge to centrifuge the enzymolysis solution to take the supernatant, use an alkaline solution to adjust the pH value to 6, and then use a potassium chloride salt solution to salt out the collagen;

[0072] Step 4: Take the collagen, put it into a dialysis bag, and use the method of gradient dialysis to dissolve it in acetic acid solution for dialysis, including the following steps;

[0073] S1-1: Put 10g of collagen into a dialysis bag with a diameter of 6cm and a length of 40cm, and seal the port of the dialysis bag;

[0074] S1-2: The first gradient dialysis, using pH = 1.5 acetic acid solution for 3 days, the temperature is 10°C, and the dialysate is changed once a day;

[0075] S1-3: The se...

Embodiment 3

[0080] Step 1: Purchase commercially available soluble collagen, put it into a dialysis bag, and use gradient dialysis to dissolve and dialysis in acetic acid solution, including the following steps;

[0081] S1-1: Put 8g of collagen into a dialysis bag with a diameter of 5cm and a length of 45cm, and seal the port of the dialysis bag;

[0082] S1-2: The first gradient dialysis, use pH=2.5 acetic acid solution for 7 days, the temperature is 15°C, change the dialysate twice a day;

[0083] S1-3: The second gradient dialysis, use pH=4 acetic acid solution for 5 days, the temperature is 15°C, and the dialysate is changed twice a day;

[0084] S1-4: The third gradient dialysis, use neutral purified water for 2 days, the temperature is 15 ℃, change the dialysate twice a day.

[0085] Step 2, take a circular stainless steel mesh tank mold with a diameter of 20cm, spread a layer of silk on the bottom surface, and then inject the collagen solution obtained from dialysis into the mold...

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Abstract

The present invention provides a preparation method of high-strength bionic collagen membrane. The preparation method is by conducting gradient dialysis process on collagen to rearrange collagen molecules into an ordered structure, and endow the molecules with more consistent and more extensive collagen fiber orientation, thus improving the mechanical properties of the material. The collagen membrane prepared by using the method has similar appearance, structure and performance to natural dura and amnion of mammals, and good rehydration, and is free to bend and fold after water absorption; the bionic collagen membrane with high tensile strength can be stitched by using suture and can be used in clinical guided tissue regeneration or repair of various tissues.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a high-strength bionic collagen film and a preparation method thereof. The material is used for repairing dura mater and dura mater defects, and can also be used for guiding tissues in tendon suture or nerve suture growth, and can also be used to induce periodontal tissue regeneration or induce bone tissue regeneration. Background technique [0002] The dura mater is the outermost layer of the membrane structure wrapped around the brain and spinal cord. It is a relatively strong connective tissue film. The natural dura mater structure is mainly composed of fibroblasts and collagen fibers, which can well protect the brain and prevent The role of cerebrospinal fluid leakage. At present, there are mainly several types of materials used in clinical dural defect repair: autologous fascia tissue, allogeneic tissue, xenogeneic biological materials, artificial synthetic materials, etc....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L31/04C08J5/18
Inventor 张自强李海涛符斌其他发明人请求不公开姓名
Owner BEIJING PAISHENG BIOTECH CO LTD
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