Vero cell domestication method suitable for serum-free culture system and application of vero cell domestication method

A serum-free culture and cell technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as instability, unclear components of bovine serum, and affecting the stability of vaccine products
CN104862270AActive Publication Date: 2015-08-26GUANGDONG WENS DAHUANONG BIOTECH
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG WENS DAHUANONG BIOTECH
Publication Date
2015-08-26

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Abstract

The invention discloses a vero cell domestication method suitable for a serum-free culture system. The vero cell domestication method comprises the following steps: reviving frozen vero cells; cultivating in a serum culture medium; and domesticating the vero cells by virtue of a domestication method of gradually reducing the serum content. The vero cells are domesticated and cultivated by virtue of the domestication method of gradually reducing the serum content, so that the vero cells can adapt to the serum-free culture environment; the vero cells which can adapt to the serum-free culture are obtained; and the domesticated vero cells can be continuously subcultured for at least 50 generations in a VP-SFM (serum-free medium).
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Description

technical field

[0001] The invention relates to the field of veterinary biological products, and more specifically relates to a vero cell domestication method suitable for a serum-free culture system and its application in a porcine epidemic diarrhea virus propagation and culture method. Background technique

[0002] At present, in the production process of domestic animal vaccines, the proliferation and culture of passaged cells and viruses mostly contain bovine serum. Because bovine serum is a natural medium, its source is limited, and its price has been rising year after year, which increases the cost of raw materials for vaccine production; the composition of bovine serum is unclear and unstable, and the differences between batches affect the stability of vaccine products; in addition, There are certain risks in the serum supply chain, and there is a potential risk of foreign pathogen contamination. The serum-free (VP-SFM) culture system has no animal-derived components...

Claims

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