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Dark black chafer luring agent composition and application thereof

A technology of black beetle and composition, applied in the direction of application, attracting pests, biocides, etc., can solve the problems of high replacement cost, low sustainability, and difficulty in popularization, and achieve strong autonomy, low price, and cost reduction Effect

Active Publication Date: 2015-11-18
PHEROBIO TECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main ingredients are L-isoleucine methyl ester and linalool. The trapping effect is good, but due to the low sustainability, it causes high replacement costs and is difficult to popularize.

Method used

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  • Dark black chafer luring agent composition and application thereof
  • Dark black chafer luring agent composition and application thereof
  • Dark black chafer luring agent composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The Y-type olfactometer of embodiment 1 single-component synergist is measured

[0032] In a room with clean air at 25°C, a relative humidity of about 70%, use a 3cm*20cm Y-shaped olfactometer, and according to the characteristics of nocturnal sexual activities of scarab beetles, conduct biometric measurements at 20:00-23:00, using red Light source observation measurement results. Take 20 μl of the single-component compound of the synergist and drop it on a filter paper strip, and place it in one side arm of a Y-shaped olfactometer, and put an equal amount of n-hexane in the other side arm as a control. Insert a dark gill beetle fasting for 24 hours at a distance of 3 cm from the main arm of the Y-shaped olfactory instrument. If the beetle stays in any side arm for more than 1 min, it is considered to have made a choice; if it stays at the main arm for 10 min, then Considered unresponsive. Repeat for 30 dark gill beetles, 15 males and 15 males. The t-test was perform...

Embodiment 2

[0039] Example 2 Determination of the antennal potential activity of the black beetle to 6-methyl-5-hepten-2-one

[0040] Prepare 6-methyl-5-hepten-2-one into 0.01, 0.1, 1, 10, 100 μg / μL n-hexane solutions for use. Use tweezers to carefully remove the antennae of the black beetle, stick the antennae on the conductive rod with conductive glue, and keep the gills of the antennae open. The mouth of the air supply tube is perpendicular to the antennae, and the distance from the antennae is about 1cm. Take 10 μL of the solution and drop it on a 2cm*0.5cm filter paper sheet. reaction on the instrument. Before the treatment, do a control with the same amount of n-hexane. The test concentration ranged from low to high, and 6 antennae (3 male and 3 antennae) were used for each test concentration, and the test was repeated 3 times for each antennae. During the test, the stimulation time was 0.5s, the interval between two times was not less than 1min, and the carrier gas flow rate was...

Embodiment 3

[0042] Example 3 Determination of the antennal potential activity of the black beetle to 2-ethyl-1-hexanol

[0043] Prepare 2-ethyl-1-hexanol into 0.01, 0.1, 1, 10, 100 μg / μL n-hexane solutions for use. Use tweezers to carefully remove the antennae of the black beetle, stick the antennae on the conductive rod with conductive glue, and keep the gills of the antennae open. The mouth of the air supply tube is perpendicular to the antennae, and the distance from the antennae is about 1cm. Take 10 μL of the solution and drop it on a 2cm*0.5cm filter paper sheet. reaction on the instrument. Before the treatment, do a control with the same amount of n-hexane. The test concentration ranged from low to high, and 6 antennae (3 male and 3 antennae) were used for each test concentration, and the test was repeated 3 times for each antennae. During the test, the stimulation time was 0.5s, the interval between two times was not less than 1min, and the carrier gas flow rate was 20mL / min. ...

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Abstract

The invention discloses a dark black chafer luring agent composition and application thereof and belongs to the technical field of insect sex pheromone preparation. The dark black chafer luring agent composition comprises a dark black chafer sex pheromone and a synergist, wherein the weight ratio of the dark black chafer sex pheromone to the synergist is 1: (0.5-100), the main components of the dark black chafer sex pheromone include isoleucinate and R-(-)-linalool, and the synergist is at least one of 6-methyl-5-heptylen-2-ketone, 2-ethyl-1-hexyl alcohol, octyl aldehyde and nonanal. The dark black chafer luring agent composition can be accurately applied to observation and prediction of the dark black chafer situation, large-number dark black chafer entrapment and disturbed mating and has important significance on comprehensive dark black chafer control.

Description

technical field [0001] The invention relates to the technical field of preparation of insect sex pheromones, in particular to a black beetle attractant composition and application thereof. Background technique [0002] The dark gill beetle (Holotrichiaparallela), also known as Lachnostemamorose Waterhouse, belongs to Coleoptera (Coleoptera), Scarabidae (Scarabeidae), is an important worldwide underground pest, especially in China, India and Japan. Both adults and larvae of the black beetle can cause serious damage. Adults feed on the leaves of trees and shrubs such as elms, poplars, pears, and apples, and have the characteristics of overeating on fixed trees and sections of fixed trees. They also eat leaves of crops such as peanuts, soybeans, corn, and sunflowers. The larvae mainly damage field crops such as peanuts, soybeans, sweet potatoes, and wheat, causing heavy losses, and in severe cases, even crop failure. [0003] The dark gill beetle is one of the three dominant ...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01P19/00A01N31/02A01N35/02
Inventor 李克斌尹姣梅向东张帅曹雅忠费仁雷刘丹丹张开心衣建坤孙庆胜
Owner PHEROBIO TECHNOLOGY CO LTD
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