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Preparation method of low-concentration IAA and glycerogel

A technology of glycerol gel and preparation method, applied in the field of plant biology, can solve problems such as high cost, loss of branches, death, etc., and achieve the effects of easy operation and reduction of water evaporation

Inactive Publication Date: 2015-11-25
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] A method for preparing low-concentration IAA and glycerin gel, characterized in that the method includes hormone mother solution preparation, working solution preparation, and hormone gel preparation, and the specific steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it with a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0013] ②Preparation of working solution: Dilute the above mother solution into a low-concentration hormone working solution with a concentration of 10mg / L according to the volume ratio of 1:100, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone and glycerol working solution with a concentration of 10 mg / L in a water bath at 50°C for later use.

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Abstract

The invention discloses a preparation method of low-concentration IAA and glycerogel and belongs to the technical field of plant biology. The preparation method includes: preparing an IAA mother solution of 1mg / mL; diluting the mother solution to be a low-concentration IAA and glycerol working solution of 10mg / L; using the working solution to prepare a low-concentration IAA and glycerol blocking solution containing 1% sodium carboxymethylcellulose and 8% sodium alginate.

Description

technical field [0001] The invention discloses a method for preparing low-concentration IAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G1/00A01G7/06
Inventor 钱夕刚
Owner 钱夕刚