Portable fluorescence quantitative PCR detector

Abstract

The invention discloses a portable fluorescence quantitative PCR detector, and belongs to the fields of biology and medical detection. The detector comprises an optical excitation unit, an optical detection unit, and a temperature control unit. The optical excitation unit comprises a plurality of light sources, an exciting light optical filter, and a holophote. The temperature control unit comprises a reaction tank, a heat cover, a heating / refrigerating sheet, a heat dissipating part, and a temperature control circuit. The optical detection unit comprises a plurality of light splitting sheets, an optical filter, an imaging sensor, and a detection circuit. The light given off by the light source goes through the exciting light optical filter and then is converted into exciting light with a specific wavelength; then the exciting light is reflected by the holophote and is projected into a PCR tube to excite the fluorescence substances in the reagent, thus the fluorescence substances will give off light, part of the light goes though the hole in the side surface of the reaction tank and enters the optical detection unit, the light is split and filtered in the optical detection unit, and finally the light form an image on the corresponding image sensor.

Description

technical field [0001] The invention relates to a portable fluorescent quantitative PCR detector, which belongs to the field of biological and medical detection. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. Real-time fluorescent quantitative PCR technology refers to the method of adding fluorescent groups to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally performing quantitative analysis of unknown templates through standard curves. [0003] At present, among the fluorescent quantitative PCR detectors on the market, the biggest difference between each product lies in the optical excitation and detection modules. These products mainly have the following deficiencies: [0004] 1. Some fluorescent quantitative PCR detectors use photodiodes as detectors, and each photodiode can only detect one sample at a t...

AI Technical Summary

Problems solved by technology

[0004] 1. Some fluorescent quantitative PCR detectors use photodiodes as detectors, and each photodiode can only detect one sample at a time, so all samples need to be scanned by mechanical scanning. The existence of these mechanical devices increases volume and power consumption and reduces reliability , more importantly, there is a difference in detection time between different samples, which affects the accuracy of the experiment, such as the CFX96Touch fluorescence quantitative PCR instrument of BIO-RAD company;

[0005] 2. The optical path design of some fluorescence quantitative PCR detectors is very complicated, which increases the volume and cost of the product and reduces the reliability, such as the TOpticalThermocycler fluorescence quantitative PCR instrument of Biometra;

[0006] 3. Some fluorescent quantitative PCR detectors achieve the purpose of multi-light path detection by adding rotatable excitation light filter color wheels and emission light filter color wheels in the light path. The existence of these mechanical devices increases power consumption and reduces Reliability, such as Roche's LightCycler480 fluorescent quantitative PCR instrument;

[0007] 4. In addition, most of the fluorescent quantitative PCR detectors currently on the market are medium and large machines for laboratory testing, which are expensive and poor in portability. Users must take the prepared samples to the laboratory for testing. This method affects the rapid diagnosis of some sudden diseases

Images