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Indoor biological measuring method of wheat aphids

A bioassay and wheat aphid technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of wheat seedlings being unable to stretch, strong escape, and the impact of bioassay results, so as to save space, ensure accuracy, reduce Effects of damage and errors

Active Publication Date: 2015-12-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet this technical scheme has taken the method for taking root and protecting absorbent cotton, to play moisturizing effect, but this technical scheme is that the wheat seedlings are curled up and placed in the petri dish, and the wheat seedlings cannot be stretched, and it is an open environment, in view of the wheat aphid is relatively The more active test insects have strong escape ability, and the open environment also has a certain impact on the bioassay results

Method used

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  • Indoor biological measuring method of wheat aphids
  • Indoor biological measuring method of wheat aphids
  • Indoor biological measuring method of wheat aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Establishment of Sensitive Toxicity Baseline of Sulfacamid to Aphid graminearum

[0033] 1. Research object

[0034] A population of A. graminearum that has not been exposed to any pesticides for many years was raised in the laboratory.

[0035] 2. Method

[0036] The test agent was sulfoxaflor original drug with a concentration greater than 95%, and the toxicity test was carried out indoors by using the plastic dropper blade film method.

[0037] 2.1 Preparation and dilution of the mother liquor of the original drug

[0038] Dissolve the original drug in acetone, store it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C. Before the toxicity test, it was diluted to various concentrations with distilled water containing 0.01% (v / v) TritonX-100 and set aside.

[0039] 2.2 Selection and use of bioassay leaves

[0040] Use indoor hydroponics to cultivate fresh wheat seedlings for about 10 days. The wheat...

Embodiment 2

[0050] 1. Research object

[0051] Laboratory-reared multigenerational field populations of wheat aphids.

[0052] 2. Method

[0053] For the test agent, sulfoxaflor, the toxicity test was carried out in the laboratory by using the method of drug film on the leaves of twelve-well cell culture plates.

[0054] 2.1 Preparation and dilution of the mother liquor of the original drug

[0055]Dissolve the original drug in acetone, store it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C. Before the toxicity test, it was diluted to various concentrations with distilled water containing 0.01% (v / v) TritonX-100 and set aside.

[0056] 2.2 Selection and use of bioassay leaves

[0057] Use indoor hydroponics to cultivate fresh wheat seedlings for about 10 days, and cut the wheat seedlings to a length of about 10 cm with clean scissors. Immerse the wheat seedlings in the dilution concentration of each drug for 15 seconds, take ...

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Abstract

The invention relates to the field of plant protection and particularly provides an indoor biological measuring method of wheat aphids. In the invention, a plastic dropper is employed as a biological measuring tool and is sealed by cotton. The method is simple in operation, can save space and is reduced in damage and error on to-be-test aphids due to operations in a biological measuring process. In addition, wheat seedlings are arranged in the plastic dropper so that the moisture of the wheat seedlings can be maintained well, thereby ensuring accuracy of a biological measuring result. The novel indoor medicine film biological measuring method of the wheat aphids can be used for finding resistance level and distribution thereof of field population of the wheat aphids on medicines in different action mechanisms, thereby guiding field application of the medicines more scientifically.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a method for indoor bioassay of wheat aphids. Background technique [0002] Wheat aphid is one of the important pests that harm wheat crops worldwide. It occurs in both northern and southern wheat regions of my country. Wheat aphids can not only directly suck plant nutrients, but also excrete a large amount of honeydew that seriously affects the respiration and photosynthesis of wheat seedlings. It is also an important medium for spreading plant viruses, causing major economic losses to agricultural production. At present, wheat aphids are mainly controlled by chemical methods, but due to the short life cycle of wheat aphids, large and fast reproduction, many times of control, large dosage and unreasonable use of single agents, wheat aphids have different degrees of resistance to some commonly used pesticides. drug resistance. [0003] Pest resistance monitoring is an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085
Inventor 高希武范银君刘晓岚于文鑫
Owner CHINA AGRI UNIV
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