Recombinant protein for diagnosing coenurosis cerebralis of sheep

A technology of recombinant protein and polycephalus, which is applied in the direction of recombinant DNA technology, microbiological determination/inspection, and the introduction of foreign genetic material using vectors, etc., which can solve the problems of limited antigen source, low specificity, and low sensitivity of diagnosis

Active Publication Date: 2015-12-16
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] Early reports of diagnostic antigens for polycephalic eczema in sheep include crude antigens such as cyst fluid and protoscolene tissue homogenate. There is cross-reaction among tapeworm cercariasis (eg, sheep thin neck cysticercosis, sheep cysticercosis)

Method used

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  • Recombinant protein for diagnosing coenurosis cerebralis of sheep
  • Recombinant protein for diagnosing coenurosis cerebralis of sheep
  • Recombinant protein for diagnosing coenurosis cerebralis of sheep

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Embodiment Construction

[0014] The present invention is described in further detail below in conjunction with embodiment.

[0015] 1. Cloning of TmAdh3 gene of brain polycephalus

[0016] 1. Genomic DNA Extraction

[0017] The sheep with cerebral polycephalus were collected from Jingtai slaughterhouse in Gansu Province, and brain polycephalic cysts were isolated by craniotomy. After confirmation by morphological examination, the original scoliosis was stripped off under aseptic conditions, washed with PBS, and finally applied with Beijing Tiangen Co., Ltd. The tissue DNA extraction kit is used to extract the genomic DNA of brain polycephales, and the specific operation is detailed in the instructions (omitted).

[0018] 2. Amplification of TmAdh3 gene

[0019] Using the brain polycephalus genomic DNA extracted in 1.1 above as a template, PCR amplification was performed with the upstream primer sequence SEQIDNo. get. PCR reaction system: 5 μL of 10×PCR buffer, 4 μL of dNTP mixture (2.5 mmol / L), 1 ...

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Abstract

The invention discloses a recombinant protein for diagnosing coenurosis cerebralis of sheep. The amino acid sequence of the recombinant protein is represented by SEQ ID No.1, the gene sequence of the recombinant protein is represented by SEQ ID No.2, and the preservation number of the recombinant protein is M2015388. The invention also discloses an upstream primer and a downstream used for synthesizing the recombinant protein and respectively represented by SEQ ID No.3 and SEQ ID No.4, and a preparation method for synthesizing the recombinant protein. A result of west-blotern and ELISA detection shows that the recombinant protein has obvious response to coenurosis cerebralis infected serum of sheep, so the recombinant protein has a diagnosis value in the coenurosis cerebralis of sheep.

Description

technical field [0001] The invention relates to a recombinant protein derived from Taenia polycephala and a preparation method thereof, and also relates to the application of the recombinant protein in the diagnosis of polycephalic eczema of sheep brain. Background technique [0002] Cerebral polycephalus ( Coenurus cerebralis ) is commonly known as cerebral hydatid disease, which is the larva of Taenia polycephala, which parasitizes in the brains of ungulate ruminants such as sheep and goats, and can cause a serious and fatal parasitic disease. Humans can also be infected as an intermediate host . Its adult worms live in the small intestine of canine carnivores. The lethality rate of cerebral polycephalus can reach 100%, which has caused huge economic losses to animal husbandry production. The disease has a global distribution and is an important zoonotic parasitic disease. Therefore, the early diagnosis of the disease has very important social and economic significance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C12Q1/68C12N15/11C12N15/70G01N33/68
CPCC07K14/00C12Q1/6893G01N33/56905G01N33/68
Inventor 李文卉张念章闫鸿斌李立岳龙付宝权
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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