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Root dipping method of low concentration BA and glycerin gel

A glycerin gel, low-concentration technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, unsatisfactory production effect, etc., and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2015-12-23
何其家
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A low-concentration BA and glycerin gel root sticking method, characterized in that the method includes hormone mother liquor preparation, working fluid preparation, hormone gel preparation, root sticking, cuttings, and the specific operation steps are as follows:

[0014] ①Preparation of hormone mother liquid: accurately weigh 0.1g of BA and dissolve it with a little 1M KOH, and dilute it to 100mL with distilled water, which is 1mg / mL BA mother liquid;

[0015] ②Working solution preparation: Dilute the above-mentioned mother liquor into a low-concentration hormone working solution with a concentration of 10mg / L according to a volume ratio of 1:100, and add 10ml glycerin according to 90ml hormone working solution at the same time;

[0016] ③Preparation of hormone gel: accurately weigh out 1g sodium carboxymethylcellulose and 8g sodium alginate, and dissolve them with 100mL of 10mg / L hormone and glycerol working solution under 50℃ water bath conditions;

[0017] Root dip: dip th...

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PUM

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Abstract

The invention discloses a root dipping method of low concentration BA and glycerin gel, and belongs to the technical field of plant biology. The method comprises the following steps: at first, preparing a BA mother liquor with a concentration of 1 mg / mL, diluting the mother liquid into a low concentration BA work fluid with a concentration of 10 mg / L, using the work fluid to prepare a low concentration BA and glycerin gel confining liquid, which contains sodium carboxymethyl cellulose with a mass concentration of 1% and sodium alginate with a mass concentration of 8%, dipping the lower ends of healthy and robust branches into the gel confining liquid for 3 to 5 seconds, taking out the branches, and inserting the branches into a cultivation medium to make the branches grow out roots.

Description

technical field [0001] The invention discloses a root dipping method of low-concentration BA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as BA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/90A01P21/00A01G1/00A01G7/06
Inventor 何其家
Owner 何其家