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Method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides

A technology of isatidis and polysaccharides, which is applied in the field of simultaneous extraction of isatidis polypeptides and polysaccharides, can solve problems such as unclear side effects, and achieve the effect of simple operation and high extraction yield

Inactive Publication Date: 2016-01-13
SUZHOU COSMETIC MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a traditional Chinese medicine, studying its main active ingredients and separating different ingredients to study their efficacy can solve the problem of unclear side effects commonly found in traditional Chinese medicine.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for simultaneously extracting polypeptides and polysaccharides from Radix Isatidis, includes the following steps:

[0026] 1) Crush 5g of Radix isatidis, soak in 25mL ethyl acetate for 3h, filter, rinse the filter cake 3 times with distilled water, and dry;

[0027] 2) Soak the dried filter cake in step 1) in 18mL distilled water for 1h, and ultrasonicate for 10s;

[0028] 3) Heat the solution after step 2) ultrasonic treatment to 60°C, stir for 3 hours at a stirring speed of 120 rpm, filter, and collect the filtrate;

[0029] 4) Soak the filtered cake of step 3) with 18 mL of distilled water for 1 hour, heat to 90°C, adjust the pH of the solution to 8.0 with sodium bicarbonate, stir for 1 hour at a stirring speed of 160 rpm, filter, and collect the filtrate;

[0030] 5) Soak the filtered cake in step 4) with 18 mL of distilled water for 1 hour, heat to 90°C, adjust the pH of the solution to 6.0 with carbonic acid, stir for 1 hour at a stirring speed of 160 rpm, filter,...

Embodiment 2

[0033] A process for simultaneously extracting polypeptides and polysaccharides from Radix Isatidis, includes the following steps:

[0034] 1) Crush 5g of Isatis root, soak in 30mL ethyl acetate for 8h, filter, rinse the filter cake with distilled water 3~5 times, and dry;

[0035] 2) Soak the dried filter cake in step 1) with 22mL of distilled water for 1.5h, and ultrasound for 40s;

[0036] 3) Heat the solution after step 2) ultrasonic treatment to 80°C, stir for 2h at a stirring speed of 200rpm, filter, and collect the filtrate;

[0037] 4) Soak the filtered cake in step 3) with 22 distilled water for 1.5 hours, heat to 80°C, adjust the pH of the solution to 8 with sodium bicarbonate, stir for 2 hours, stir at 200 rpm, filter, and collect the filtrate;

[0038] 5) Soak the filtered cake in step 4) with 22 distilled water for 1.5 hours, heat to 80°C, adjust the pH of the solution to 6 with carbonic acid, stir for 2 hours, stir at 200 rpm, filter, and collect the filtrate;

[0039] 6) C...

Embodiment 3

[0041] A process for simultaneously extracting polypeptides and polysaccharides from Radix Isatidis, includes the following steps:

[0042] 1) Take 5g of Radix isatidis and crush it, soak it in 40mL ethyl acetate for 5h, filter, rinse the filter cake 5 times with distilled water, and dry;

[0043] 2) Soak the dried filter cake in step 1) with 15mL of distilled water for 2h, and ultrasonic for 40s;

[0044] 3) Heat the solution after step 2) ultrasonic treatment to 80°C, stir for 2h at 180rpm, filter and collect the filtrate;

[0045] 4) Soak the filter cake filtered in step 3) with 15 mL of distilled water for 2 hours, heat to 80°C, adjust the pH of the solution to 8 with sodium bicarbonate, stir for 2 hours, stir at 180 rpm, filter, and collect the filtrate;

[0046] 5) Soak the filtered cake in step 4) with 15 mL of distilled water for 2 hours, heat to 80°C, adjust the pH of the solution to 6 with carbonic acid, stir for 2 hours, stir at 180 rpm, filter, and collect the filtrate;

[00...

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PUM

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Abstract

The invention discloses a method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides. The method comprises following steps: radix isatidis is smashed, and is immersed in ethyl acetate, an obtained solution is filtered, and an obtained filter cake is washed with distilled water for 3 to 5 times, and is dried; the filter cake is immersed in distilled water for 1 to 2h, and is subjected to ultrasonic treatment for 10 to 80s; an obtained mixed solution is heated to 60 to 90 DEG C, is stirred for 1 to 3h at a stirring speed of 120 to 300rpm, and is filtered, and a filtrate is collected; an obtained filter cake is immersed in distilled water for 1 to 2h, is heated, and is subjected to acid alkali adjustment respectively, an obtained mixture is stirred for 1 to 3h at a stirring speed of 120 to 300rpm, and is filtered, and a filtrate is collected; the filtrates obtained via the above steps are mixed, and an obtained mixed material is delivered into an evaporating dish for evaporation and concentration, ethyl alcohol is added, an obtained product is allowed to stand for 12 to 24h, and is subjected to centrifugation, a supernatant is discarded, an obtained precipitate is collected, and is subjected to vacuum drying so as to obtain a polypeptide and polysaccharide mixture. The method can be used for simultaneous extraction of radix isatidis polypeptides and polysaccharides; operation is simple; polypeptides extraction rate is as high as 95.45%; polysaccharides extraction rate is as high as 92.48%; and impurity content is less than 5%.

Description

Technical field [0001] The invention belongs to the technical field of polypeptide extraction, and specifically relates to a process for simultaneously extracting Isatis root polypeptide and polysaccharide. Background technique [0002] Banlangen is a commonly used Chinese medicine. Bitter taste, cold in nature, has the functions of clearing away heat and toxins, cooling blood and throat. It is used for diseases such as plague poisoning, high fever headache, big head plague, rotten throat pill, cheek, throat numbness, sore swelling, chicken pox, measles, hepatitis, influenza and other diseases. As a traditional Chinese medicine, studying its main active ingredients and separating different ingredients to study their efficacy can solve the problem of unclear side effects that are common in traditional Chinese medicine. According to reports in the literature, the chemical components contained in Radix Isatidis mainly fall into five categories: alkaloids, mustard, sulfur-containin...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/30C08B37/00
Inventor 金仲恩全春兰张帆喻国贞欧阳智琨陈晋纳
Owner SUZHOU COSMETIC MATERIALS
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