Lentinula edodes strain medium and preparing method thereof
A culture medium for bacteria and shiitake mushroom technology, which is applied in the field of agricultural breeding, can solve the problems of polluting the environment, wasting reusable resources, etc., and achieves the effects of simple preparation method, strong anti-bacteria ability, and avoiding environmental pollution
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Embodiment 1
[0012] A culture medium for shiitake mushrooms, prepared from the following raw materials in parts by weight: 20 parts of sweet potato powder, 50 parts of dried chestnut powder, 2 parts of lead acetate powder, 20 parts of beef peptone, 2 parts of potassium dihydrogen phosphate, and 1 part of magnesium sulfate , 5 parts of corn flour.
[0013] A preparation method of mushroom culture medium, comprising the following steps:
[0014] (1) Put the dry powder of chestnut branches into warm water at 55°C, the quality of the warm water is ten times the weight of the dry powder of chestnut branches, soak for 6 hours, cool it to 20-25°C, filter it with gauze to remove the slag, and obtain the filtrate;
[0015] (2) Use 2% sodium hydroxide solution in the obtained filtrate to adjust the filtrate to a pH value of 8, then weigh other raw materials except chestnut branch dry powder according to the above weight parts, add them to the filtrate in turn, stir well, and place overnight;
[00...
Embodiment 2
[0018] A culture medium for mushroom strains, prepared from the following raw materials in parts by weight: 22 parts of sweet potato powder, 48 parts of dried chestnut powder, 3 parts of lead acetate powder, 22 parts of beef peptone, 2 parts of potassium dihydrogen phosphate, and 1 part of magnesium sulfate , 2 parts of corn flour.
[0019] A preparation method of mushroom culture medium, comprising the following steps:
[0020] (1) Put the dried chestnut branch powder into warm water at 58°C, the quality of the warm water is ten times the weight of the dried chestnut branch powder, soak for 5 hours, cool it to 20-25°C, filter it with gauze to remove the slag, and obtain the filtrate;
[0021] (2) Use 2% sodium hydroxide solution in the obtained filtrate to adjust the filtrate to a pH value of 8, then weigh other raw materials except chestnut branch dry powder according to the above weight parts, add them to the filtrate in turn, stir well, and place overnight;
[0022] (3) ...
Embodiment 3
[0024] A culture medium for shiitake mushrooms, prepared from the following raw materials in parts by weight: 25 parts of sweet potato powder, 45 parts of dried chestnut powder, 2 parts of lead acetate powder, 20 parts of beef peptone, 3 parts of potassium dihydrogen phosphate, and 2 parts of magnesium sulfate , 3 parts of corn flour.
[0025] A preparation method of mushroom culture medium, comprising the following steps:
[0026] (1) Put the dry powder of chestnut branches into warm water at 60°C, the quality of the warm water is ten times the weight of the dry powder of chestnut branches, soak for 4 hours, cool it to 20-25°C, filter it with gauze to remove the slag, and obtain the filtrate;
[0027] (2) Use 2% sodium hydroxide solution in the obtained filtrate to adjust the filtrate to a pH value of 8, then weigh other raw materials except chestnut branch dry powder according to the above weight parts, add them to the filtrate in turn, stir well, and place overnight;
[0...
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