Regulating agent based on licorice extract, its preparation method and application
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A technology of licorice extract and regulating agent, applied in application, animal feed, animal feed, etc., can solve the problems of inability to enhance the immunity of farmed fish, high production cost and breeding cost, high price of pure glycyrrhetinic acid, and achieve Enhance immunity, regulate fat, and eliminate the effect of high prices
Active Publication Date: 2018-11-23
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0007] (1) During the breeding process, fish fatty liver is often accompanied by a decline in immunity. Only using pure glycyrrhetinic acid to regulate fat cannot enhance the immunity of farmed fish and has little effect on the survival rate;
[0008] (2) Glycyrrhetinic acid is used alone, the price of pure glycyrrhetinic acid is high, and the source is unstable, resulting in high production costs and breeding costs, low economic benefits, and is not conducive to the sustainable development of farmers
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preparation example Construction
[0030] The preparation process of licorice extract is as follows:
[0031] 1) Weigh 100.0 g of licorice and chop it into pieces, add 800.00 g of 10% ethanol aqueous solution containing 0.50% ammonia at a ratio of 1:6.5 to 8.5, and extract twice at 80°C under reflux. Use a flask of not less than 1L for the extraction device, filter for 2 hours each time, filter with a vacuum pump, and use a filter cloth of appropriate specifications;
[0032] 2) Combine the two extracts, concentrate in vacuum to 1 / 8~1 / 10 of the original volume, add the diluted concentrated sulfuric acid GR V:deionized water V=1:1 dropwise under vigorous stirring, and adjust the pH to 1.5-2.0, stand for 5 hours, filter under reduced pressure, wash the filter cake with water to pH 3.5-4.0, and dry to obtain the crude product 1;
[0033] 3) Add 100 ml of 6-8% sulfuric acid aqueous solution to the crude product 1 extracted in the above steps, heat to boiling at 100°C under normal pressure, and reflux for 12 h with...
Embodiment 1
[0037] Example 1 of the regulator of the present invention is as follows: 1 part of licorice extract, 4 parts of potassium chloride, 1 part of citric acid, 1 part of ethoxyquinoline, and 1 part of zeolite powder.
[0038] 2. The application of regulators
[0039] The regulating agent of the present invention can be applied to the preparation of fish feed for reducing body fat of fish, and the regulating agent can be added in a ratio of 0.3-0.5 g / kg to any fish feed.
[0040] Embodiment 1 of the present invention:
[0041]Fish feed components: 5 parts of fish meal, 30 parts of soybean meal, 12.5 parts of rapeseed meal, 15 parts of cotton meal, 2.26 parts of soybean oil, 0 part of flour, 4.5 parts of bran, 1.8 parts of calcium dihydrogen phosphate, 1 part of compound premix and Regulator 2 parts. These components were thoroughly mixed to prepare fish feed. The regulating agent that adopts is the prescription of above-mentioned regulating agent embodiment 1.
[0042] Experime...
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Abstract
The invention belongs to the technical fields of animal breeding, nutrient adjusting and controlling and disease prevention, and particularly relates to an adjusting and controlling agent using a licorice root extract as a main material as well as a preparation method and application of the adjusting and controlling agent. The adjusting and controlling agent is prepared from the following components in parts by weight: 1-3 parts of a licorice root extract, 2-4 parts of potassium chloride, 1-3 parts of citric acid, 1-3 parts of ethoxyquin, and 1-3 parts of zeolite powder. The adjusting and controlling agent is prepared through the following steps of respectively crushing the raw materials into 120-mesh granules, thoroughly mixing the granules to obtain a mixture, and putting the mixture under 23-27 DEG C for stirring for 24 hours so as to obtain glycyrrhetinic acid; thoroughly mixing the mixture of the glycyrrhetinic acid and glycyrrhizinic acid in the proportion of the glycyrrhetinic acid to the glycyrrhizinic acid being (1-2.5) to (1-1.5 ) with 2-4 parts of potassium chloride so as to obtain a new mixture; and putting the mixture under room-temperature environment for 24 hours or more, so as to obtain an off-white mixture. The off-white mixture can be used as a fat adjusting and controlling agent for fish. According to the adjusting and controlling agent disclosed by the invention, the improvement is made, the licorice root extract is used for replacing pure glycyrrhetinic acid products, and the potassium chloride is used for replacing sodium chloride, so that the immunity of fish bodies is further improved from many aspects.
Description
technical field [0001] The invention belongs to the technical field of animal breeding, nutrition regulation and disease prevention, in particular to a regulating agent mainly composed of licorice extract, its preparation method and application. Background technique [0002] Studies have shown that in today's aquaculture process, due to problems such as feed nutrient imbalance, excessive fat and energy added to feed, water quality deterioration, and various stress sources, the fat deposition in the liver and viscera of farmed fish increases, and the muscle fat content changes. , resulting in frequent fatty liver of fish, reduced initial catch rate, decreased immunity, decreased anti-stress ability, decreased survival rate, decreased fish quality, etc., seriously affecting the healthy development of aquaculture industry and the supply of green aquatic products. At the same time, in order to reduce losses, some farmers have abused antibiotics and fishery medicines, resulting i...
Claims
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Application Information
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