Walnut oligopeptide powder, and preparation method and application thereof

A technology of walnut oligopeptide and walnut peptide is applied in the field of preparation and enzymolysis, and can solve the problems of easy blockage of ultrafiltration membrane, difficulty in large-scale production, and reduction of polypeptide yield.

Pending Publication Date: 2020-09-08
SINPHAR TIAN LI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patent involves the use of a supercritical extractor when removing grease, which increases the cost and is not easy for large-scale production; it involves 4 kinds of enzymatic hydrolysis in four steps, the steps are cumbersome and the cost is high; it involves no microfiltration after the enzymatic hydrolysis is completed Membrane removal of impurities, direct ultrafiltration membrane, resulting in easy clogging of the ultrafiltration membrane, prolonged ultrafiltration time, reducing the yield of polypeptides, and shortening the service life of the ultrafiltration membrane; this patent does not involve the percentage of peptides and peptides in peptide products molecular weight distribution
[0009] It can be seen that the preparation conditions of the above walnut polypeptides are harsh, the process steps are cumbersome, and it is difficult to achieve large-scale production. Therefore, finding a set of preparation methods for walnut polypeptides with simple process, low cost, high content, and high activity has become a search for large-scale production. One of the research hotspots

Method used

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  • Walnut oligopeptide powder, and preparation method and application thereof
  • Walnut oligopeptide powder, and preparation method and application thereof
  • Walnut oligopeptide powder, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0039] Mix 100kg of cold-pressed and degreased walnut meal (recorded as A) with water at a weight ratio of 1:10, adjust the pH to 10, and extract at room temperature for 2 hours; The same amount of walnut meal (recorded as B), adjust the pH to 10, and extract at room temperature for 2 hours; after B completes the first extraction, the filtrate is set aside, and the filter residue continues to be extracted for the second time; after A completes the second extraction, the filter residue is discarded , the filtrate was poured into an equivalent amount of walnut meal (recorded as C), adjusted to pH 10 and extracted at room temperature for 2 hours; after B completed the second extraction, the filter residue was discarded, and the filtrate was poured into C to complete the first extraction of the filter residue for 2 hours. And the filtrate extracted for the first time of C is for use; after the second extraction of sample C, the filter residue is discarded, and the filtrate is for use...

preparation Embodiment 2

[0043] Mix 100kg of cold-pressed and degreased walnut meal (recorded as A) with water at a weight ratio of 1:10, adjust the pH to 10, and extract at room temperature for 2 hours; The same amount of walnut meal (recorded as B), adjust the pH to 10, and extract at room temperature for 2 hours; after B completes the first extraction, the filtrate is set aside, and the filter residue continues to be extracted for the second time; after A completes the second extraction, the filter residue is discarded , the filtrate was poured into an equivalent amount of walnut meal (recorded as C), adjusted to pH 10 and extracted at room temperature for 2 hours; after B completed the second extraction, the filter residue was discarded, and the filtrate was poured into C to complete the first extraction of the filter residue for 2 hours. And the filtrate extracted for the first time of C is for use; after the second extraction of sample C, the filter residue is discarded, and the filtrate is for u...

Embodiment 1

[0047] Evaluation of effect of walnut peptide on sedation and hypnosis to improve sleep

[0048] 1.1 Experimental scheme:

[0049] Randomly select normal wild-type AB strain zebrafish 5 days after fertilization (5dpf) in a six-well plate, 30 fish per well (ie, each experimental group), and give walnut oligopeptides of 222 μg / mL, 667 μg / mL, and 2000 μg respectively in water. / mL, positive control drug oxazepam 100 μ M concentration, set the normal control group (fish culture water treatment zebrafish) and model control group at the same time, the capacity of each well (experimental group) is 3mL. After 24 hours of treatment with the test product, transfer the zebrafish to a 96-well plate, one fish per well (that is, each concentration group). Except for the normal control group, the rest of the experimental groups were induced with PTZ (pentylenetetrazole) to establish an insomnia model. 10 zebrafish were randomly selected in each experimental group, and the insomnia time and ...

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Abstract

The invention relates to walnut oligopeptide powder, and a preparation method and application thereof. The peptide content in the oligopeptide powder is 80wt% or above, and the molecular weight of 95%or more of walnut peptide is less than 1500 Dalton. The method comprises the following steps: extracting walnut proteins by using a high-efficiency countercurrent extraction method, performing filtering, performing enzymolysis, performing high-efficiency separation and purification on the obtained protein enzymatic hydrolysate through a microfiltration membrane and an ultrafiltration membrane insequence, and finally performing concentrating and spraying to obtain the oligopeptide powder. The walnut oligopeptide powder has the effects of improving sleep, protecting alcoholic fatty liver, inhibiting fat absorption, regulating the pH value of intestinal tracts to improve beneficial flora, and enhancing nutrient absorption.

Description

technical field [0001] The invention relates to a high-purity, low-molecular-weight walnut oligopeptide product. The invention also relates to the preparation and enzymatic hydrolysis of walnut peptide to produce oligomeric walnut peptide powder. The peptide powder can be used as food, health food or medicine. Background technique [0002] Walnut (Juglans regia L), also known as walnut, belongs to one of the four major nuts and has high nutritional and medicinal value. In ancient medical books in my country, there are clear records, Li Shizhen's "Compendium of Materia Medica" records "replenishing qi and nourishing blood, moistening dryness and resolving phlegm, benefiting the gate of life, benefiting the triple burner, warming the lungs and intestines. Treating the lungs and intestines. Treating deficiency and cold, asthma and cough , severe pain in the waist and feet, confidant colic, bloody dysentery and intestinal wind, dispelling swelling and poison..." Song Liuhan's "K...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34A23L33/18A23J3/34A23J1/14A61K38/01A61P25/20A61P1/00A61K36/52
CPCC12P21/06C07K1/145C07K1/34A23L33/18A23J3/346A23J1/14A61K38/011A61P25/20A61P1/00A23V2002/00A23V2200/30A23V2250/55
Inventor 王昭日杨进平崔玉梅李易达
Owner SINPHAR TIAN LI PHARMA
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