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Preparation method of protein self-assembly artificial liver support

An artificial liver and self-assembly technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of rejection, high technical difficulty and high cost, and achieve the effect of improving accuracy, reducing rejection, and reducing consumables.

Inactive Publication Date: 2016-05-11
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

But so far, medical students mainly use traditional technology when preparing hybrid AL, which has a series of problems such as technical difficulty, long development time cycle, and high cost.
[0006] Therefore, in order to overcome the shortcomings of traditional technologies such as poor biocompatibility and rejection reactions, and combine advanced bioprinting technology, the present invention takes the lead in using silk protein as a scaffold material, using protein self-assembly characteristics and combining advanced bioprinting technology to prepare Methods of Liver Stenting

Method used

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Embodiment Construction

[0028] Describe the present invention below in conjunction with specific embodiment:

[0029] The preparation method of the protein self-assembled artificial liver scaffold in this embodiment comprises the following steps:

[0030] Step 1: Prepare printing materials:

[0031] Step 1.1: Expose silk to CaCl 2 Dissolve in the aqueous solution to obtain the dissolving treatment solution. In order to make full use of the silk, in this embodiment the silk is cut into fragments with the largest size less than 2mm, and then placed in CaCl 2 Dissolve in aqueous solution; filter the solution to obtain fibroin / CaCl 2 Mixed aqueous solution; for silk protein / CaCl 2 The mixed aqueous solution is placed in a dialysis bag for dialysis purification with water to obtain a fibroin solution; and the concentration of the fibroin solution is adjusted to 40mg / ml-80mg / ml, preferably 60mg / ml in this embodiment.

[0032] Step 1.2: subculture the patient's healthy hepatocytes; in this embodiment, t...

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Abstract

The invention provides a preparation method of a protein self-assembly artificial liver support. The method comprises the steps that a three-dimensional model of the liver of a patient is established according to the individual characteristics of the liver of the patient, and then a prepared fibroin solution with a certain concentration and liver cells, subjected to in-vitro subculturing, of the patient are evenly mixed to be a printing material; the mixed printing material is added into a biological forming machine; printing is conducted in an external ultraviolet irradiation environment finally, and preparation of the liver support is achieved. According to the method, the self-assembly characteristic of protein is utilized, an advanced biological printing technology is combined, and therefore preparation of the artificial liver support on the basis of the protein is achieved; the method can not only improve the preparation precision of the liver support, but also has higher biocompatibility.

Description

technical field [0001] The invention relates to a method for preparing an artificial liver scaffold based on protein self-assembly properties. It involves the technical field of cell culture, the technical field of protein self-assembly characteristics, and the technical field of bioprinted organs. Background technique [0002] Protein is an organic macromolecular substance, which is necessary to maintain life activities and growth, and is also one of the commonly used materials for liver tissue engineering. The self-assembly behavior of protein molecules refers to the spontaneous formation of ordered structures between specific protein structural units. Protein molecules exhibit highly ordered self-assembly properties during their formation. The self-assembly behavior of proteins is an important guarantee and driving force for maintaining the normal operation of life systems and promoting the evolution of life forms. Protein denaturation refers to the unstable conformati...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/38
CPCA61L27/225A61L27/3804A61L27/3839A61L2430/28C08L89/00
Inventor 汪焰恩王淑志魏庆华李欣培杨明明柴卫红
Owner NORTHWESTERN POLYTECHNICAL UNIV
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