Staining module for biological samples on glass slide and staining method thereof

Abstract

The invention relates to a dyeing module applied to biological samples on glass slides and a dyeing method thereof. The dyeing module comprises a shell with an opening in the upper part and a module cover articulated with the shell, wherein a slicing frame is transversely arranged in the shell; a plurality of spring clips used for horizontally clamping glass slides are arranged on the slicing frame along a length direction at equal intervals; a plurality of module tanks are arranged in the shell along a length direction at equal intervals; a plurality of drainage holes are also formed in one side of each module tank; the drainage holes are communicated with a drainage tank beside the module tanks; the drainage tank is extended to the bottom of the shell; a drainage valve is arranged at the outlet of the drainage tank; a heating plate used for heating reagents is closely attached to the bottom of the shell; a fan is also arranged under the heating plate; the heating plate, the fan and the drainage valve are controlled by a control unit. The dyeing module applied to biological samples on glass slides has the beneficial effects that the flexibility is high, the reagents are reduced, and the reliability and stability of dyeing result can be ensured.

Description

technical field [0001] The invention relates to the field of medical instruments and scientific instruments, in particular to the technical field of immunohistochemistry and immunocytochemistry staining, in particular to a staining module for biological samples on glass slides and a staining method thereof. Background technique [0002] 1. Overview of immunohistochemistry automation [0003] Since the United States Technicon successfully developed the world's first fully automatic biochemical analyzer in 1957, a variety of fully automatic biochemical analyzers with different models and functions have emerged one after another, bringing good news to tedious clinical biochemical test workers. Greatly improved work efficiency and inspection accuracy. Since the liquid phase-liquid phase reaction technology in biochemical analysis is relatively simple and mature, and the key details of the control required are few, the fully automatic development is relatively rapid. However, i...

AI Technical Summary

Problems solved by technology

After the incubation is completed, the liquid in the gap between the slides is blotted dry with an absorbent pad. Each reagent and washing step is completed by filling and drying the capillary. The main defect of this technique is that the capillary movement in the gap between the slides is very slow Much depends on the surface tension of the liquid, and the thickness, folding, and rolling of tissue sections will also affect the gap between slides or between coverslips and slides, so reagents may cover unevenly, resulting in uneven or weakened staining

[0008] 3. Challenges faced by immunohistochemical automation

[0033] (1) The flexibility of reagent selection is poor;

[0034] (2) The flexibility of antigen retrieval operation is relatively poor, and the antigen retrieval effect is usually not as good as manual;

[0035] (3) The reagent consumption is relatively large;

[0036] (4) Higher cost;

[0037] (5) Regular maintenance is required;

However, the more factors an instrument can change, the less consistent, stable and reliable staining results will be

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