Bacterial magnetic particle expressing recombinant protein g and its application

A technology of bacterial magnetic particles and recombinant proteins, which is applied in the application of nano magnetic beads and medical testing, can solve the problems of increasing the cost of anti-human ball tests, and achieve the effects of improving stability, improving amplification ability, and enhancing load capacity

Active Publication Date: 2017-12-08
BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In automated detection, the anti-human globulin test needs to be used in combination with micro-column gel cards. As the cost of micro-column gel cards becomes higher and higher, the anti-human globulin test in routine tests and blood group antibody screening increases to a certain extent. cost of testing

Method used

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  • Bacterial magnetic particle expressing recombinant protein g and its application
  • Bacterial magnetic particle expressing recombinant protein g and its application
  • Bacterial magnetic particle expressing recombinant protein g and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Bacterial Magnetic Particles Expressing Recombinant Protein G

[0049] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, separating and purifying the recombinant strain of magnetotactic bacterium MSR-1.

Embodiment 2

[0050] Example 2 Bacterial Magnetic Particles Expressing Recombinant Protein G

[0051] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, separating and purifying the recombinant strain of magnetotactic bacterium MSR-1;

[0052] The sequence of the recombinant protein G is:

[0053] The magnetotactic bacteria MSR-1 recombinant strain is constructed by the following method:

[0054] a. Construction of a mutant strain of magnetotactic bacteria MSR-1 with deletion of the bacterial magnetic particle membrane protein mamC / mamF gene;

[0055] b. Construction of bacterial magnetic particle membrane protein fusion expression plasmid;

[0056] c. Transfer the functionalized bacterial magnetic particle fusion expression plasmid into the mutant strain of magnetotactic bacteria MSR-1 prepared in step a to construct a recombinant strain of magnetotactic bacteria MSR-1. ...

Embodiment 3

[0057] Example 3 Bacterial Magnetic Particles Expressing Recombinant Protein G

[0058] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, separating and purifying the recombinant strain of magnetotactic bacterium MSR-1;

[0059] The sequence of the recombinant protein G is:

[0060] The preparation method of the bacterial magnetic particles expressing recombinant protein G is:

[0061] a. Construction of a mutant strain of magnetotactic bacteria MSR-1 with deletion of the bacterial magnetic particle membrane protein mamC / mamF gene;

[0062] (a) Amplify two homologous fragments with a length of 700-1000 bp on the left and right sides of the bacterial magnetic particle membrane protein mamC / mamF gene; both left and right homologous fragments have two restriction sites;

[0063] (b) The left and right homologous fragments are double-digested with corresponding...

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Abstract

The invention provides bacterial magnetic particles with recombinant protein G expression capability. The bacterial magnetic particle with recombinant protein G expression capability are obtained by performing enlarged culture and separation and purification on magnetotactic bacteria MSR-1 recombinant strains. The invention further provides application of the bacterial magnetic particles with recombinant protein G expression capability in detection of blood type irregular antibody IgG. By use of the magnetic property of the bacterial magnetic particles and the effect of an applied magnetic field, the bacterial magnetic particles with recombinant protein G expression capability, provided by the invention, are capable of assisting agglutination of sensitized erythrocytes, so that on the one hand, the usage amount of the bacterial magnetic particles is reduced to the greatest extent, and on the other hand, the detection sensitivity of low-potency IgG antibodies is improved to the greatest extent.

Description

technical field [0001] The invention relates to the fields of application of nanometer magnetic beads and medical detection, in particular to bacterial magnetic particles expressing recombinant protein G and applications thereof. Background technique [0002] Bacterial magnetic particles are a kind of magnetic nanoparticles produced by magnetotactic bacteria, and the core is Fe 3 o 4 The crystal is coated with a layer of phospholipid biofilm outside, and the particle size is between 30-120nm. The bacterial magnetic particles produced by the same magnetotactic bacteria have basically the same particle size and crystal form, uniform magnetic properties, natural biofilm coating, and good water-soluble and colloidal properties. In addition, because the bacterial magnetic particles are of biological origin, they have good biocompatibility. There are a large number of amino groups on the bacterial magnetic particle membrane, which can be connected with different functional macr...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/21G01N33/80G01N33/68G01N33/554C12N15/74
CPCC07K14/315C12N15/74G01N33/554G01N33/6854G01N33/80G01N2333/315
Inventor张金菊王红光
OwnerBEIJING GUO KE RONG ZHI BIOTECH CO LTD