Preparation method of renal biopsy electron microscopy sample

An electron microscope sample and biopsy technology, which is applied in the field of kidney biopsy electron microscope sample preparation, can solve the problems of wrong specimens, difficulty in locating glomeruli, waste of manpower and material resources, etc., to reduce time-consuming positioning, improve diagnosis rate and accuracy of diagnosis Degree, the effect of reducing labor costs

Inactive Publication Date: 2016-05-25
CENT SOUTH UNIV
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Problems solved by technology

At present, renal biopsy electron microscopy has become one of the routine examination methods for the research and diagnosis of renal diseases, especially playing an irreplaceable role in the classification of primary glomerular diseases. For example, the tissue is skin or fat, or medulla without balls, etc.), as well as subsequent blind sample preparation of puncture specimens or routine

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Embodiment Construction

[0013] The present invention will be described in further detail below.

[0014] Move the fixed renal puncture tissue submitted for electron microscope diagnosis to a clean glass slide, place it under a microscope with a light source, select a 2.5x objective lens, and turn the condenser to the highest position, first determine whether the specimen is kidney tissue, If it is kidney tissue, judge whether the specimen has glomeruli and the number and distribution of glomeruli. Then use a double-sided razor blade to cut out kidney tissue segments with glomeruli, each segment is about 1-3mm in length, paying attention to focus on cutting out glomerulus-dense areas as much as possible. Draw and mark the tissue section with the ball for later use (this operation should be done quickly, and the tissue specimen should be moisturized with physiological saline or fixative solution to prevent drying). If the optical permeability of the specimen is poor, the specimen can be turned over fo...

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Abstract

The invention discloses a preparation method of a renal biopsy electron microscopy sample. The method comprises the following steps: transferring fixed renal biopsy tissues to a slide glass, placing the slide glass under a microscope with a light source, and cutting the tissues by using a double side blade to form a renal tissue segment with the length of 1-3mm and with glomerulus; cleaning the cut renal tissue, dehydrating the cleaned renal tissue, and immersing the dehydrated renal tissue; embedding the renal tissue; carrying out block trimming and positioning on the above obtained embedded and clustered block sample; accurately trimming and positioning the sample according to an electron microscopy observation result; and cutting the sample through using an ultrathin slicer to obtain an ultrathin slice. The preparation method of the renal biopsy electron microscopy sample allows adverse misbipsy samples to be found in the early stage and to be timely corrected, so repeated sample production is avoided; and the method also has the advantages of fast block trimming, short positioning time, accurate positioning, effective shortening of the sample preparation time, and substantial improvement of the diagnosable rate of renal biopsy samples and the diagnosis precision.

Description

technical field [0001] The invention relates to a method for preparing a kidney biopsy electron microscope sample. Background technique [0002] Renal biopsy is percutaneous renal biopsy, which is not only an important branch of pathology, but also an indispensable part of nephrology. Renal biopsy is very important for understanding the pathological changes, pathological types, and progression of kidney diseases and continuously optimizing individualized treatment plans. , Judging the prognosis is of great significance. In the 1950s, the application of electron microscopy in renal biopsy, especially transmission electron microscopy, allowed the observation of pathological changes in the kidney to the subcellular level. At present, renal biopsy electron microscopy has become one of the routine examination methods for the research and diagnosis of renal diseases, especially playing an irreplaceable role in the classification of primary glomerular diseases. For example, the t...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
Inventor 吴晓英
Owner CENT SOUTH UNIV
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