A rice pollen-specific expression promoter ospoll2 and its application
A pollen-specific, promoter technology, applied in the fields of biotechnology and crop genetic engineering, can solve the problems of the target gene expression time or space cannot be well controlled, the improvement effect is not obvious, and the plant growth and development is high.
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[0059] In this embodiment, the present invention utilizes the above-mentioned rice pollen-specific expression promoter OsPoll2 to drive the antisense fragment of the OsRIP gene (LOC_Os12g03822).
[0060] First, using a method similar to the above, the rice pollen-specific expression promoter OsPoll2 is first connected to the upstream of the antisense fragment of the target gene to obtain a crop expression vector in which the promoter OsPoll2 is fused with the antisense fragment of the target gene.
[0061] Next, the combination product of the rice pollen-specific expression promoter OsPoll2 and the antisense fragment of the target gene was transferred into Agrobacterium tumefaciens. The seeds of the plant of interest are transformed with Agrobacterium tumefaciens transformed with the conjugated product. Finally, the transformed seeds were used to cultivate the corresponding plants. In the obtained plants, it was found by potassium iodide staining that part of the pollen was ab...
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