Rice pollen specific expression promoter OsPoll2 and application thereof

Abstract

The invention provides a rice pollen specific expression promoter OsPoll2 and application thereof. The invention further provides an expression box and a recombinant expression vector containing the promoter; besides, the invention further provides a method for driving special purpose gene expression through the rice pollen specific expression promoter OsPoll2 in pollen of plants. The promoter is applied to crop genetic engineering. The promoter can specifically drive the target gene to be expressed in crop pollen, so that activity of the pollen can effectively changed, and the risk of transgenosis drifting caused by diffusion of active pollen is eliminated. The promoter is utilized in cooperation with the target gene with a pollen activity inhibition function to improve an existing transgenosis rice variety, excellent characters on other aspects of transgenosis rice are retained, the risk of gene drifting is also avoided, a good foundation is laid for deep research and application and popularization of the transgenosis variety, and effective safe guarantee is provided.

Description

Technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice pollen-specific expression promoter and its application. The promoter can drive the specific expression of target genes in pollen in crop genetic engineering, thereby effectively preventing and controlling transgene drift by changing pollen vigor. Background technique [0002] Transgenic technology is considered to be one of the most effective ways for humans to solve the contradiction between the sharp decline of land resources and the rapid expansion of population. However, due to the short development and utilization of this technology, humans have very limited understanding of its biological safety. And among the public, many people have concerns about the safety of plant genetically modified technology. Exogenous genes carried by transgenic plants may drift with pollen to closely related species or wild spe...

AI Technical Summary

Problems solved by technology

[0005] At present, some constitutive strong promoters are widely used in the field of agricultural biotechnology, such as the CaMV35S promoter and the corn Ubiquitin-1 promoter. However, when using these promoters to induce the target gene to transform rice and other crops to improve crop quality , the improvement effect is often not obvious because the time or space of the expression of the target gene cannot be well controlled, or the growth and development of the plant are affected because the gene expression induced by these constitutive promoters is too high

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