A method for separating tangeretin and nobiletin from citrus flavonoids extract

A technology of citrus flavonoids and nobiletin, applied in the direction of organic chemistry, can solve the problems of difficult to obtain market recognition for quality, difficult to achieve mass production, complex production process, etc., to achieve large-scale mass production, low cost, and extraction efficiency high effect

Active Publication Date: 2018-03-30
MIANYANG DIAO PHARMA
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Problems solved by technology

Therefore, its production process is very complicated, the production cost is extremely high, it is difficult to achieve mass production, and because it is a mixture, it is difficult to be recognized by the market in terms of quality

Method used

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Embodiment

[0034] The invention provides a method for separating tangeretin and nobiletin from citrus flavonoids extract, wherein the method for separating tangeretin is as follows:

[0035] (1) Grinding Fructus Fructus Citrus Fructus or Tangerine Peel to obtain the crushed raw material, then adding the first organic solvent with a concentration of 30-50%, and under the reflux condition of 80-90°C, reflux extracting the crushed raw material for 2-3 hours to obtain Extraction solution; in the present embodiment, the first organic solvent is any one in methanol, ethanol, ethyl acetate, acetone and n-butanol;

[0036] (2) adding the first organic solvent with a concentration of 30-50% to mix again, and then extracting the pulverized raw materials for 2-3 hours under the reflux condition of 80-90° C. to obtain a secondary extract, and mix it with the first The extracts are combined;

[0037] (3) extracting solution is recovered to obtain the crude extract of citrus flavonoid mixture;

[00...

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Abstract

The invention discloses a method for separating tangeretin and nobiletin from citrus flavonoid extract, comprising the main steps: crushing immature bitter orange or orange peel, adding a first organic solvent, and refluxing for extracting to obtain first extract liquid; adding the first organic solvent again, refluxing for extracting to obtain second extract liquid, and combining the second extract liquid with the first extract liquid; recycling the extract liquid to obtain crude extract; adding clear water, mixing to obtain a mixed solution, and adding a second organic solvent for extracting to obtain a water layer and an organic layer; recycling the organic layer to obtain extract; adding a third organic solvent and activated carbon; extracting a mixture, subjecting extraction liquid to cooling and filtering to obtain filtrate and activated carbon residue, vacuum concentrating the filtrate to obtain secondary extract; adding ethyl alcohol to dissolve the secondary extract, and separating and refining to obtain tangeretin; dissolving the activated carbon residue with ethyl alcohol, and acquiring secondary extract by cooling, filtering and ethyl alcohol recycling; adding ethyl alcohol for reflux dissolving, and separating and refining to obtain nobiletin. The problem that it is difficult for the prior art to acquire high content of polymethoxyflavonoids and the component is single is solved.

Description

technical field [0001] The invention relates to the technical field of extract separation, in particular to a method for separating tangeretin and nobiletin from citrus flavone extracts. Background technique [0002] Fat-soluble citrus bioflavonoids mainly refer to citrus-specific polymethoxyflavonoids (PMFs), which have stronger anticancer activity than general flavonoids. Ligands of polymethoxyflavonoids such as nobiletin (nobiletin), tangeretin (also known as tangeretin, tangeretin) and sweet orange flavonoid (sinensetin), although the raw material content is very low, but because it has more than general flavonoids Stronger physiological activity, thus attracting more attention. At present, it has been confirmed that its anti-tumor effect is much stronger than that of general flavonoids. After research, the main reason is that polymethoxy flavonoids are fat-soluble, and they are easier to access and penetrate into tumor cells to play a role. Specifically, nobiletin (no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 周传武
Owner MIANYANG DIAO PHARMA
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