Application of mtDNA copy number to evaluation of coronary artery bypass transplanting postoperative new onset atrial fibrillation

A technology of coronary artery and copy number, applied in the field of medical bioengineering

Inactive Publication Date: 2016-07-06
张建
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its purpose is to solve the current biological diagnosis of new-onset atrial fibrillation after off-pump coronary artery bypass grafting

Method used

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  • Application of mtDNA copy number to evaluation of coronary artery bypass transplanting postoperative new onset atrial fibrillation
  • Application of mtDNA copy number to evaluation of coronary artery bypass transplanting postoperative new onset atrial fibrillation
  • Application of mtDNA copy number to evaluation of coronary artery bypass transplanting postoperative new onset atrial fibrillation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Mitochondrial DNA copy number expression in peripheral blood of patients undergoing off-pump coronary artery bypass grafting

[0029] From January 2015 to June 2015, 335 patients who underwent off-pump coronary artery bypass grafting were selected in the Department of Cardiovascular Surgery, General Hospital of Shenyang Military Region. 3 ml of peripheral blood was collected before operation, and whole blood genome extraction kit ( SangonBiotech), carried out real-time quantitative PCR on the extracted genome to detect the copy number expression of mitochondrial DNA in the genome.

[0030] The specific method is as follows: the mitochondrial DNA copy number in the preoperative whole blood of patients planning to undergo off-pump coronary artery bypass grafting was detected by real-time quantitative PCR. The peripheral blood whole genome extraction kit (SangonBiotech) was used, and the primers were designed as follows: ND1-F5'-CCCTAAAAACCCGCCACATCT-3'; ND1-R

...

Embodiment 2

[0035] Example 2 Chi-square test analysis of the correlation between mitochondrial DNA copy number and clinical data

[0036] In the patient of Example 1, the expression of copy number of mitochondrial DNA was analyzed, and the median Ct value of 17.16 was taken as the cut-off point. If the value was higher than 17.16, the copy number of mitochondrial DNA was increased, and if it was lower than 17.16, the copy number of mitochondrial DNA was decreased.

[0037] Table 1 Correlation analysis between mitochondrial DNA copy number and clinical data

[0038]

[0039]

[0040] The results showed that the mitochondrial DNA copy number in patients before off-pump coronary artery bypass grafting was significantly correlated with patient age and postoperative atrial fibrillation.

Embodiment 3

[0041] Example 3 Logistic Regression Analysis Factors Affecting Atrial Fibrillation After Off-pump Coronary Artery Bypass Grafting

[0042] Among the patients in Example 1, binary Logistic regression analysis was performed using SPSS18.0 analysis software.

[0043] Table 2. Univariate and multivariate Logistic regression analysis

[0044]

[0045]

[0046] The results showed that preoperative mitochondrial DNA copy number in peripheral blood was an independent risk factor for atrial fibrillation after off-pump coronary artery bypass grafting.

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Abstract

The invention provides a method for assessing new-onset atrial fibrillation after coronary artery bypass grafting under off-pump coronary artery bypass grafting by using the copy number of mitochondrial DNA in peripheral blood. The steps of the method are as follows: detecting the mitochondrial DNA copy number in the blood of the preoperative coronary artery bypass grafting patient by real-time quantitative PCR, and according to the quantitative PCR result, it is possible to judge the occurrence of postoperative new atrial fibrillation in the patient and guide the coronary artery bypass grafting Individualized treatment of postoperative patients.

Description

technical field [0001] The invention belongs to the technical field of medical bioengineering. Specifically, the present invention relates to the use of mitochondrial DNA copy number (mtDNA copy number) in circulating blood to evaluate the marker application of new-onset atrial fibrillation after coronary artery bypass grafting under off-pump coronary artery bypass grafting. Background technique [0002] Postoperative new-onset atrial fibrillation is the most common complication after coronary artery bypass grafting, with an incidence rate between 15-50%. Postoperative new-onset atrial fibrillation is closely related to the long-term mortality of patients. It has been found that advanced age, race, structural cardiomyopathy, metabolic syndrome, and obesity are all associated with postoperative AF. Studies have reported that the expression of ROS production-related enzymes (NAPDH oxidase) in the atrial tissue of patients with postoperative atrial fibrillation was significan...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/118C12Q2600/158
Inventor张建王辉山徐银丽徐殊
Owner张建