Application of isorhamnetin to measurement of copper ions

A technology of isorhamnetin and copper ions, which is applied in the field of optical analysis and detection, can solve the problems of less research on fluorescence performance and unreported detection research, and achieve the effects of high sensitivity, low interference and low detection limit

Inactive Publication Date: 2016-07-06
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Isorhamnetin has various biological activities such as anti-oxidation, anti-inflammation, and anti-tumor, but there are few studies on its fluorescence properties, and the research on its detection of metal ions as a fluorescent probe has not been reported yet.

Method used

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  • Application of isorhamnetin to measurement of copper ions
  • Application of isorhamnetin to measurement of copper ions
  • Application of isorhamnetin to measurement of copper ions

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The application of isorhamnetin in measuring copper ions comprises the following steps:

[0022] (1) Accurately weigh 0.0316g of isorhamnetin, dilute it to a 10mL volumetric flask with methanol, and make a concentration of 1×10 -2 mol L -1 Solution, take 0.1mL and dilute to 100mL volumetric flask with PBS buffer solution (pH=7.40) to make 1×10 -5 mol L -1 Solution, ready for use; accurately weigh 0.0200g Cu(AC) 2 ·H 2 Dilute O to a 10mL volumetric flask with water to make a solution containing copper ions with a concentration of 1×10 -2 mol L -1 solution, and successively diluted to 1×10 -3 , 1×10 -4 , 1×10 -5 mol L -1 solution, ready to use.

[0023] (2) Prepare several 5mL dry and clean sample tubes, add 2mL1×10 -5 mol L -1 The isorhamnetin solution, and the copper ion solution with different volumes and different concentrations, the concentration of isorhamnetin is 1 × 10 -5 mol L -1 , the copper ion concentration is 0×10 -7 mol L -1 , 0.1×10 -7 mol ...

Embodiment 2

[0031] Containing isorhamnetin 1 x 10 -5 mol L -1 In the buffer, add Li-containing + 、Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ 、Cu 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3+ 、Eu 3+ 、Gd 3+ 、Dy 3+ acetate solution, where Li + 、Na + 、K + , Mg 2+ , Ca 2+ Concentration is 5 times of copper ion concentration, Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3+ 、Eu 3+ 、Gd 3+ 、Dy 3+ Concentration is 2 times of copper ion concentration, and the fluorescent intensity measured under the same condition and method of embodiment 1 is as follows image 3 As shown (other unmarked metal ions in the figure are from left to right: Li + 、Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3 + 、Eu 3+ 、Gd 3+ 、Dy 3+ ), it can be seen from the figure that only the addition of copper ions will cause obvious fluorescence quenching, and other metal ions have litt...

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Abstract

The invention discloses an application of isorhamnetin to measurement of copper ions, and belongs to the technical field of optical analysis and detection. According to the application, a 10<-5>mol / L solution is prepared from isorhamnetin by virtue of a CH3OH-PBS buffer solution with pH of 7.40, copper ions are added to form different concentrations, fluorescence intensity changes are measured by adopting fluorescence spectroscopy, a standard curve of the copper ion concentrations at different fluorescence intensities is drawn, and the copper ion concentration of an unknown sample is calculated according to the standard curve. A measurement process has the characteristics of being simple, sensitive, accurate, environmentally friendly, and the like, and is high in practicability.

Description

technical field [0001] The invention belongs to the technical field of optical analysis and detection, and in particular relates to the application of isorhamnetin in the determination of copper ions. Background technique [0002] Copper widely exists in nature and the human body, and is an indispensable trace element for the human body. At the same time, copper is widely used in metal smelting, electronics, packaging and other industries closely related to human life. With the accelerated development of human society, mineral resources The phenomenon of indiscriminate mining is becoming more and more serious. In addition to the three industrial wastes, domestic garbage and electronic waste are also increasing, which eventually leads to the loss of heavy metal ions, resulting in excessive heavy metal ion content in soil and water. Although copper is an essential trace element for the human body, if the content of copper in the body is too high, it will cause gastrointestinal...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 徐莉杨世龙姜维娜赵俸艺
Owner NANJING FORESTRY UNIV
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