A strain of Aspergillus terreus and its application in the preparation of xylanase

A technology of xylanase and Aspergillus terreus, applied in the direction of glycosylase, enzyme, hydrolase, etc., to achieve selective and unique selective effect

Active Publication Date: 2020-02-07
广西多得乐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aspergillus terreus is a commonly used strain for producing lovastatin and phytase in industry, but there are relatively few reports on the production of xylanase by Aspergillus terreus, and xylanase produced by Aspergillus terreus can hydrolyze xylan, and the resulting product Mainly xylobiose has not been reported yet

Method used

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  • A strain of Aspergillus terreus and its application in the preparation of xylanase
  • A strain of Aspergillus terreus and its application in the preparation of xylanase
  • A strain of Aspergillus terreus and its application in the preparation of xylanase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, acquisition and identification of Aspergillus terreus strain FC7-1

[0033] 1. Obtaining the strain

[0034] (1) Collection of soil samples: The soil in a shallow soil layer of 8-20 cm in a nature reserve in Guangxi, China was collected.

[0035] (2) Isolation and screening of bacterial strains

[0036] 1. prepare screening medium with distilled water; Contain in every liter of screening medium: bagasse xylan 10g, NaNO 3 2g, KH 2 PO 4 1g, FeSO 4 ·7H 2 O 0.001g, MgSO 4 ·7H 2 O 0.5g, agar 15g; pH 7.0; add water to 1L, sterilize with moist heat at 121°C for 20min, mix well, pour plate.

[0037] ②Take 1g of soil sample and put it into a 150mL Erlenmeyer flask, add 19mL of sterile water, stir on a magnetic stirrer for 30min, take the suspension for gradient dilution (10 -1 、10 -2 、10 -3 、10 -4 and 10 -5 ), 100 μL each was spread on a screening medium plate, and cultured at 28°C.

[0038] ③After 5 days, observe the growth of the colonies, ...

Embodiment 2

[0052] 1. Obtaining xylanase preparation

[0053] (1) Preparation of fermentation medium: the fermentation medium of every L is composed of 5g yeast powder, 5g peptone, 30g corn cob, 0.1g MgSO 4 ·7H 2 O, 0.1g CaCl 2 , 1g KH 2 PO 4 , 1g Tween 80 and water, make up the volume with water.

[0054] (2) 35L fermentation medium was loaded into a 50L fermenter, sterilized at 121°C for 20min, and the Aspergillus terreus (Aspergillusterreus) strain FC7-1 was inoculated into the fermenter at an inoculum size of 1%, fermented at 26°C and 200rpm for 4 days, and fermented During the process, the pH is controlled to be 4.0, and the dissolved oxygen is controlled to be 20%. The xylanase activity of the obtained fermented liquid (ie xylanase preparation) after fermentation is 388U / mL.

[0055] 2. Enzymatic characteristics of xylanase in fermentation broth

[0056] 1. Optimum pH value and optimum temperature of xylanase in fermentation broth

[0057] 1), the most suitable pH value

[...

Embodiment 3

[0065] Embodiment 3, utilize aspergillus terreus (Aspergillus terreus) bacterial strain FC7-1 to prepare xylanase

[0066] 1. Obtaining xylanase preparation

[0067] (1) Preparation of fermentation medium: the fermentation medium of every L is composed of 10g yeast powder, 10g peptone, 40g corn cob, 0.5g MgSO 4 ·7H 2 O, 0.3g CaCl 2 , 2g KH 2 PO 4 , 2g Tween 80 and water, make up the volume with water.

[0068] (2) 35L of fermentation medium was loaded into a 50L fermenter, sterilized at 121°C for 20min, inoculated into the fermenter with an inoculum of 2% of Aspergillus sterreus strain FC7-1, fermented at 28°C and 250rpm for 5 days, and fermented During the process, the pH is controlled to be 5.0, and the dissolved oxygen is controlled to be 30%. The xylanase activity of the obtained fermented liquid (ie xylanase preparation) after fermentation is 263U / mL.

[0069] 2. Enzymatic characteristics of xylanase in fermentation broth

[0070] The optimum action pH value and t...

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PUM

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Abstract

The invention discloses a strain of Aspergillus terreus and its application in preparing xylanase. The Aspergillus terreus strain FC7-1 provided by the present invention has a preservation number of CGMCC No.11657. The present invention also provides the application of Aspergillus terreus strain in the preparation of xylanase and the method for producing xylanase, comprising the following steps: carrying out liquid fermentation of the above-mentioned (Aspergillus terreus) bacterial strain FC7-1 in a fermenter, collecting Fermentation product, the fermentation supernatant obtained after centrifugation to remove bacteria can be used as xylanase preparation, the optimum pH value of the xylanase contained in the xylanase preparation is 5.5, and the optimum temperature is 55-60°C , the main product of the hydrolysis of bagasse xylan is xylobiose. This invention is of great significance for making full use of agricultural waste, improving the comprehensive economic benefits of the sugarcane industry and reducing environmental pressure.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Aspergillus terreus strain and its application in preparing xylanase. Background technique [0002] Xylooligosaccharides are a general term for disaccharides to heptasaccharides connected by xylose units through β-1,4-glycosidic bonds. They are functional oligosaccharides and cannot be digested by the human body. Studies have found that xylooligosaccharides, especially xylobiose in them, can be used as prebiotics to stimulate the growth of probiotics in the human intestinal tract - bifidobacteria, and have a more significant effect than fructooligosaccharides or other oligosaccharides . [0003] At present, the production of xylooligosaccharide mainly includes physical chemical method and biological method. The main process of biological production of xylo-oligosaccharides is to obtain xylan by alkaline extraction, and then use xylanase to degrade xylan to produce xylo-oligosacc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/66
CPCC12N9/2482C12Y302/01008C12N1/145C12R2001/66
Inventor 杨齐黄妹平黄斌良吴华德黄燕菲蓝健益
Owner 广西多得乐生物科技有限公司
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