Open tissue culture medium and preparation method thereof for eustoma russellianum

Abstract

The invention provides an open tissue culture medium of Eustoma eustoma and a preparation method thereof. Based on the weight of 1L medium, the medium comprises the following components: 25-35g of sucrose, 6-12g of agar, 80-120g of garlic crude extract ml, macroelements 4090mg, CaCl 2 2H 2 O 440 mg, trace elements 38.23 mg; cytokinin 1 mg, iron salt 65.1 mg; organic matter 3.1 mg; creatine 100 mg. When the culture medium of the invention is used, no high pressure sterilization is needed, and no strict aseptic operation is needed to realize good growth of plants under relatively open conditions, which greatly reduces costs and is beneficial to the application and promotion of plant tissue culture technology.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an open tissue culture medium of eustoma and a preparation method thereof. Background technique [0002] Because the traditional plant tissue culture needs to be operated under strict aseptic environment conditions, the working conditions are harsh, the operation is not easy to control, and a lot of manpower and material resources are required, and the traditional tissue culture seedlings have slow growth, serious pollution, and poor survival. The low efficiency and other problems make it difficult for plant tissue culture to be widely used and promoted in actual industrial and agricultural production. The emerging open tissue culture uses specially configured medium to make tissue culture plants grow in an antibacterial environment, so that plants can grow well under relatively open conditions. It can not only reduce the cost, but also facilitate the application and popu...

AI Technical Summary

Problems solved by technology

[0002] Because the traditional plant tissue culture needs to be operated under strict aseptic environment conditions, the working conditions are harsh, the operation is not easy to control, and a lot of manpower and material resources are required, and the traditional tissue culture seedlings have slow growth, serious pollution, and poor survival. The low efficiency and other problems make it difficult for plant tissue culture to be widely used and promoted in actual industrial and agricultural production.