Open tissue culture medium and preparation method thereof for eustoma russellianum

A tissue culture medium and an open technology, which is applied in the fields of botany equipment and methods, horticultural methods, horticultural tools/equipment, etc., can solve the problems of pollution, a large amount of manpower, material resources, and the difficulty of plant tissue culture to be widely used and promoted. To achieve the effect of cost reduction and good growth

Inactive Publication Date: 2016-08-17
FOSHAN UNIVERSITY
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Problems solved by technology

[0002] Because the traditional plant tissue culture needs to be operated under strict aseptic environment conditions, the working conditions are harsh, the operation is not easy to control, and a lot of manpower and material resources are required, and ...

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Embodiment Construction

[0060] The present invention provides an eustoma open tissue culture medium and a preparation method thereof. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0061] The eustoma open tissue culture medium of the present invention, wherein, by 1L medium weight, said medium comprises the following components:

[0062] Sucrose 25-35g;

[0063] Agar 6-12g;

[0064] Garlic crude extract 80-120 ml;

[0065] Cytokinin 1mg;

[0066] Naphthaleneacetic acid 0.5mg;

[0067] KNO 3 1900mg;

[0068] NH 4 NO 3 1650 mg;

[0069] MgSO 4 ·7H 2 O 370 mg;

[0070] K H 2 PO 4 170 mg;

[0071] CaCl 2 2H 2 O 440g;

[0072] MnSO 4 4H 2 O 22.3 mg;

[0073] ZnSO 4·7H 2 O 8.6mg;

[0074] h 3 BO 3 6.2 mg;...

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Abstract

The invention provides an open tissue culture medium of Eustoma eustoma and a preparation method thereof. Based on the weight of 1L medium, the medium comprises the following components: 25-35g of sucrose, 6-12g of agar, 80-120g of garlic crude extract ml, macroelements 4090mg, CaCl 2 2H 2 O 440 mg, trace elements 38.23 mg; cytokinin 1 mg, iron salt 65.1 mg; organic matter 3.1 mg; creatine 100 mg. When the culture medium of the invention is used, no high pressure sterilization is needed, and no strict aseptic operation is needed to realize good growth of plants under relatively open conditions, which greatly reduces costs and is beneficial to the application and promotion of plant tissue culture technology.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an open tissue culture medium of eustoma and a preparation method thereof. Background technique [0002] Because the traditional plant tissue culture needs to be operated under strict aseptic environment conditions, the working conditions are harsh, the operation is not easy to control, and a lot of manpower and material resources are required, and the traditional tissue culture seedlings have slow growth, serious pollution, and poor survival. The low efficiency and other problems make it difficult for plant tissue culture to be widely used and promoted in actual industrial and agricultural production. The emerging open tissue culture uses specially configured medium to make tissue culture plants grow in an antibacterial environment, so that plants can grow well under relatively open conditions. It can not only reduce the cost, but also facilitate the application and popu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 周权驹许桂莲陈春晓杨泉女王蕴波
Owner FOSHAN UNIVERSITY
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