A Confirmatory Analytical Method for the Detection of Piperazine Residues in Poultry and Pig Tissues

An analysis method and tissue technology, which are applied in the field of confirmatory analysis for the detection of piperazine residues in poultry and pig tissues, and can solve problems such as unreported, no luminescent group in piperazine, detection and the like.

Active Publication Date: 2018-01-09
YANGZHOU UNIV
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Problems solved by technology

In liquid chromatography, piperazine has no luminescent groups and cannot be directly detected by ultraviolet detectors or fluorescence detectors. Therefore, piperazine is often derivatized with specific groups through pre-column derivatization to make detection possible.
So far, the research of gas chromatography-tandem mass spectrometry (GC-MS / MS) for the detection of piperazine residues in animal tissues by a relatively simple acetic anhydride pre-column derivatization method has not been reported at home and abroad.

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  • A Confirmatory Analytical Method for the Detection of Piperazine Residues in Poultry and Pig Tissues
  • A Confirmatory Analytical Method for the Detection of Piperazine Residues in Poultry and Pig Tissues
  • A Confirmatory Analytical Method for the Detection of Piperazine Residues in Poultry and Pig Tissues

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[0019] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0020] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. These examples are only for illustration of the present invention and do not limit the scope of the present invention in any way.

[0021] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0022] 1. Breeding and Sample Collection of Experimental Poultry and Pigs

[0023] Three-year-old three-way hybrid pigs and poultry were selected from the mar...

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Abstract

The invention relates to the field of detection of veterinary drug residues, in particular to a confirmatory analysis method for efficiently detecting piperazine residues in poultry and pig tissues (muscle, liver, kidney and sebum). In this method, tissue samples are extracted, purified and derivatized by accelerated solvent extraction, and then detected by gas chromatography-tandem mass spectrometry (GC / MS-MS). Compared with other detection methods in terms of recovery rate, precision and sensitivity, the present invention has the advantages of simplicity, speed, accuracy and sensitivity.

Description

technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to a confirmatory analysis method for detection of piperazine residues in poultry and pig tissues. Background technique [0002] Although there are many reports on piperazine residue detection methods at home and abroad, there are mainly gravimetric methods, colorimetry, non-aqueous titration, gas chromatography, high performance liquid chromatography and (ultra) high performance liquid chromatography-tandem mass spectrometry method, etc., but gravimetric method, colorimetric method and non-aqueous titration method are not suitable for the detection of piperazine in biological samples, and the sensitivity is low. In liquid chromatography, piperazine has no luminescent groups and cannot be directly detected by ultraviolet detectors or fluorescence detectors. Therefore, piperazine is often derivatized with specific groups through pre-column derivatization, thereby ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/025
Inventor 谢恺舟刘亚楠庞茂达谢星崔璐璐王波高强张杨杨张跟喜戴国俊卜仕金王冉王金玉
Owner YANGZHOU UNIV
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