33 results about "Porcine tissue" patented technology

The invention discloses a method for directly cryopreserving pig ear edge tissues by cycloastragenol and application of cycloastragenol, and relates to the technical field of refrigeration or cryopreservation. On the basis of an existing refrigerating fluid formula, the concentration of DMSO is reduced, high-glucose DMEM is used for replacing DPBS, meanwhile, trehalose, cycloastragenol and the like are added, and a brand-new refrigerating fluid formula is provided. The method has the following advantages: 1) an unfreezing solution and an equilibrium solution do not need to be additionally prepared; the unfreezing method is easy to operate, only simple melting is needed, and the method is suitable for large-scale preservation. And 3) the tissue does not need to be subjected to complex pretreatment, and only the tissue needs to be simply cleaned. The method has the advantages that the method is suitable for field sampling, strict sterile conditions are not needed, the cell survival rate, the cell apoptosis rate, the mitochondrial membrane potential, the active oxygen, the cell proliferation rate and the like are not obviously different from those of cells cultured from fresh tissue, and the volume of the supported cryopreserved tissue is the maximum cryopreserved volume in the cryopreserved pig tissue which can be found at present.

This disclosure provides compositions and methods for reducing hyperacute rejection in xenotransplantation using porcine cells including a sequence encoding human ST8Sia6. The disclosure further provides methods of producing porcine cells and porcine tissues encoding human ST8Sia6. The disclosure further provides methods of treatment using said porcine cells and porcine tissues.