30 results about "Porcine tissue" patented technology

A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg / mL.

The invention provides a Seneca valley virus SVV-ZM-201801 and an application thereof. Separation is performed from pig tissues, and sub-culture and plaque purification are performed, so that the Seneca valley virus is obtained. The separated strain can be stably proliferated on sub-culture cells, typical cytopathic effects are formed, and the virus titer is as high as 108.5-1010.5TCID50 / mL. The separated strain does not have obvious pathogenicity on piggies. The Seneca valley virus separated strain has good proliferating properties, and is good in immunogenicity. Vaccines prepared from the separated strain can induce the piggies to generate high-level neutralizing antibodies, and powerful technical support is provided for effective prevention and control of the Seneca valley virus.

The invention relates to the field of detection of veterinary drug residues, in particular to a confirmatory analysis method for efficiently detecting piperazine residues in poultry and pig tissues (muscle, liver, kidney and sebum). In this method, tissue samples are extracted, purified and derivatized by accelerated solvent extraction, and then detected by gas chromatography-tandem mass spectrometry (GC / MS-MS). Compared with other detection methods in terms of recovery rate, precision and sensitivity, the present invention has the advantages of simplicity, speed, accuracy and sensitivity.

Porcine nucleic acid sequences flanking potentially infectious porcine endogenous retroviral (PERV) insertion sites have been identified and isolated. The unique flanking sequences include porcine nucleic acid sequences that flank the 3′ end and porcine nucleic acid sequences that flank the 5′ end of PERV insertion sites. The present invention provides compositions and methods for detecting presence of PERV in a sample, particularly those with infectious potential. In addition, the invention relates to breeding of pigs or selection of porcine tissue that is free of infectious PERV for use as a xenotransplant tissue.

The present invention relates to an antigenic fusionprotein, which carries multiple Galα1,3Gal epitopes. The fusion protein according to the invention may also be comprised of a heavily glycosylated mucin part, which mediates binding to selecting, such as PSGL-1, and a part, which exhibits immunoglobulin properties, such as the Fc part of IgG. The fusionprotein according to the invention is preferably used as an absorber to prevent a hyperacute rejection of a xenotransplant, such as a pig tissue or organ transplanted into a human patient. In addition, the invention relates to a method for the prevention of a hyperacute rejection reaction in a patient who is to receive a xenotransplant.

The invention discloses a PCR primer pair for identifying or assisting identification of an animal tissue / or an organ and application thereof. The primer pair provided by the invention is at least one from five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ, wherein the PCR primer pair for identifying or assisting identification of pig tissue / or organ is always included. The five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ respectively consist of two DNA single chains shown as a sequence 1 and a sequence 2, two DNA single chains shown as a sequence 3 and a sequence 4, two DNA single chains shown as a sequence 5 and a sequence 6, two DNA single chains shown as a sequence 7 and a sequence 8, and two DNA single chains shown as a sequence 9 and a sequence 10 in the sequence table. An annealing temperature of 62 DEG C is employed for the five primer pairs to complete all PCR identifications at once and at a same temperature; besides, the PCR primer pair has strong singularity and a short detection time.

The invention discloses a pig IGFN1 protein polyclonal antibody, further discloses antigenic polypeptide for inducing the pig IGFN1 protein polyclonal antibody and belongs to the field of animal gene engineering. The antigenic polypeptide is formed by the amino acid sequence as shown by SEQ ID No: 1. The nucleotide sequence shown by SEQ ID No: 2 forms the antigenic polypeptide encoding genes. The pig IGFN1 protein polyclonal antibody is high in pig IGFN1 protein expression level detection specificity, and an important tool is provided for expression analysis and correlational studies of IGFN1 in pig tissues. The antigen sequence is long, and the detection sensitivity is high.

The invention provides a pretreatment kit for detecting ochratoxin A in pig tissue. The pretreatment kit is characterized by comprising a buffer solution, an extracting solution 1, an extracting solution 2, an IAC column, a leacheate and an elution solution. The invention also provides a method for detecting the ochratoxin A in the pig tissue by utilizing the pretreatment kit. The method is characterized in that after the pig tissue is pretreated by utilizing the kit, the ochratoxin A is quantitatively detected by utilizing liquid chromatography-mass spectrometry under a certain liquid and mass spectrometry condition. The kit and the method have characteristics of low detection limit and high sensitivity and are relatively high in stability, precision and accuracy.

The invention discloses a method for detecting a menbutone residue in pig tissue. The method comprises the steps as follows: firstly, the pig tissue subjected to intramuscular menbutone injection is weighed, cut up and then subjected to homogenate, and the tissue subjected to homogenate is extracted by using acetonitrile, then after centrifugation, the extracted liquid is subjected to rotary evaporation in vacuum with the temperature of 60 DEG C to dry, and after cooling, moving phase is added into the residue for ultraphonic dissolution and then centrifugation, and a liquid supernatant is collected and filtered by a millipore filter membrane with filtering particle diameter of 0.22 mu m, and the filter liquor is used as a test solution; secondly, a menbutone reference substance is weighed precisely, and after dissolved by acetonitrile and diluted by moving phase, the reference substance has a concentration of 9 to 11 mu g / mL, and a reference substance solution is obtained; thirdly, HPLC (High-Performance Liquid Chromatography) is adopted for testing the test solution and the reference substance solution, the chromatogram is recorded, and the menbutone residue in the tissue is calculated by peak area according to external standard method. The method is good in specificity, high in sensitivity and good in repeatability, can quickly detect the menbutone residue in the pig tissue, and is a reliable method for controlling the quantity of the menbutone residue in animal-origin food.

The invention discloses a porcine OPN monoclonal antibody, its hybridoma cell line and application. The hybridoma cell line secreting porcine OPN monoclonal antibody, which is classified as hybridoma cell line Cy2, was deposited in the China Center for Type Culture Collection (CCTCC) on December 19, 2019, and the preservation address is Wuhan, China .Wuhan University, the preservation number is CCTCC NO: C2019293. The porcine OPN monoclonal antibody is secreted and produced by the above-mentioned hybridoma cell line or its passaged cell line. The monoclonal antibody is suitable for quickly and accurately detecting the content of OPN protein in porcine tissue, semen or porcine oocytes. Therefore, the monoclonal antibody can be used to prepare related detection kits or colloidal gold test paper, etc. The in-depth research of OPN has laid a firm foundation.

The invention discloses a preparation method of multiple acellular materials modified with nucleus pulposus cells for porcine small intestinal submucosa (SIS). The method specially comprises the following steps: stripping the porcine SIS tissues; carrying out treatment on the stripped porcine SIS tissues through a normal saline buffer solution containing a protease inhibitor, an organic solvent solution, a PBS (Phosphate Buffered Saline) buffer solution containing Triton X, a PBS buffer solution containing SDS (Sodium Dodecyl Sulfate) and a PBS buffer solution containing DNA enzyme to obtain acellular SIS materials; performing ball milling and pulverization on the acellular SIS materials to obtain micro-particles with diameter of 200mu m; carrying out co-culture on the micro-particles andseparated nucleus pulposus cells and then carrying out decellularization treatment again so as to obtain injectable multiple acellular nucleus pulposus repairing materials. According to the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS disclosed by the invention, the completeness of original ECM (Extra Cellular Matrix) can be preserved while allogeneic cells or heterologous cells with immunoreactivity are removed at the same time, and the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS has an good extracellular micro-environment, bio-factors, biomechanical properties and the like, and can simulate a growth environment of nucleus pulposus cells in normalphysiological conditions to a maximum limit.

The invention relates to medicinal kallikrein (KLK1) and a preparing method and application thereof. The kallikrein does not contain KLK1c. Tissue kallikrein from porcine pancreas is composed of three components with different glycosylation degrees. A protein electrophoresis analysis result is three stripes, named KLK1b, KLK1a and KLK1c respectively in a molecular weight descending order, wherein KLK1c has the lowest glycosylation degree and the lowest specific activity. Compared with existing tissue kallikrein from porcine pancreas available in the market, the tissue kallikrein with KLK1c removed has the advantages of being higher in purity and stability, higher in biological activity, good in pharmacodynamic effect and smaller in side effect. The invention also provides a method for purifying porcine tissue kallikrein. The problem that KLK1b, KLK1a and KLK1c are hard to separate and industrialization can not be achieved is solved.

The subject of the invention is a sterile, dehydrated acellular implant, which during its rehydration by water or bodily fluids displays anisotropic expansion and can act as a substrate for adhesion, migration and growth of live cells. Collagen structures of the transplant are at least partially denatured through the action of heat or organic solvents, such as lower aliphatic alcohols and ketones, which simultaneously act as preservative and sterilization agents, especially for certain types of viruses. The implant is sterilized by radiation while in an substantially dehydrated state, preferably by accelerated electrons. The transplant can be derived from various animal tissues, especially mammalian tissues, such as human or porcine tissues. The tissues suitable for the invention can be, for example, skin, placenta, pericardium, peritoneum, intestinal wall, tendon, blood vessel, etc. The implant is suitable for use in human and veterinary medicine, for instance as a temporary wound and burn cover, for the repair, substitution and regeneration of tissues, and also as a substrate for laboratory cell cultivation.

The invention provides a Seneca valley virus SVV-ZM-201801 and an application thereof. Separation is performed from pig tissues, and sub-culture and plaque purification are performed, so that the Seneca valley virus is obtained. The separated strain can be stably proliferated on sub-culture cells, typical cytopathic effects are formed, and the virus titer is as high as 108.5-1010.5TCID50 / mL. The separated strain does not have obvious pathogenicity on piggies. The Seneca valley virus separated strain has good proliferating properties, and is good in immunogenicity. Vaccines prepared from the separated strain can induce the piggies to generate high-level neutralizing antibodies, and powerful technical support is provided for effective prevention and control of the Seneca valley virus.

The invention belongs to the field of biochemistry and molecular immunology, and relates to a preparation method and application of a porcine neurointermediate B polypeptide and its polyclonal antibody. Blue protein KLH coupling to obtain NMB modified polypeptide-KLH coupled protein; emulsify NMB modified polypeptide-KLH coupled protein and immunize New Zealand white rabbits; collect and separate serum containing rabbit anti-pig NMB modified polypeptide antibody; use Protein G to purify The prepared NMB polyclonal antibody was purified by column method. The polypeptide antigen and polyclonal antibody have the characteristics of simple preparation method, low cost and high titer, and can specifically bind NMB protein in pig tissue.

The invention discloses a method for simultaneously determining the contents of bacitracin B2 and bacitracin B3 in pig tissues, which adopts a liquid chromatography-tandem mass spectrometry method for determination, and the chromatographic conditions are as follows: a mobile phase is chromatographically pure acetonitrile and a 0.1% formic acid solution; the mobile phase ratio is as follows: the mobile phase ratio is 20% chromatographic pure acetonitrile in 0-0.2 min, the mobile phase ratio is changed from 20% chromatographic pure acetonitrile in 0.2-6.0 min to 30% chromatographic pure acetonitrile, the mobile phase ratio is changed from 30% chromatographic pure acetonitrile in 6.0-6.1 min to 80% chromatographic pure acetonitrile, the mobile phase ratio is changed from 80% chromatographic pure acetonitrile in 6.1-7.0 min, the mobile phase ratio is changed from 80% chromatographic pure acetonitrile in 7.0-7.1 min to 20% chromatographic pure acetonitrile, and the mobile phase ratio is changed from 7.1-9.0 min to 20% chromatographic pure acetonitrile; the flow velocity of the mobile phase is 0.300 mL / min; the sample size is 10.0 microliters.