31 results about "Porcine tissue" patented technology

The present invention relates to an antigenic fusionprotein, which carries multiple Galα1,3Gal epitopes. The fusion protein according to the invention may also be comprised of a heavily glycosylated mucin part, which mediates binding to selecting, such as PSGL-1, and a part, which exhibits immunoglobulin properties, such as the Fc part of IgG. The fusionprotein according to the invention is preferably used as an absorber to prevent a hyperacute rejection of a xenotransplant, such as a pig tissue or organ transplanted into a human patient. In addition, the invention relates to a method for the prevention of a hyperacute rejection reaction in a patient who is to receive a xenotransplant.

The invention discloses a method for detecting a menbutone residue in pig tissue. The method comprises the steps as follows: firstly, the pig tissue subjected to intramuscular menbutone injection is weighed, cut up and then subjected to homogenate, and the tissue subjected to homogenate is extracted by using acetonitrile, then after centrifugation, the extracted liquid is subjected to rotary evaporation in vacuum with the temperature of 60 DEG C to dry, and after cooling, moving phase is added into the residue for ultraphonic dissolution and then centrifugation, and a liquid supernatant is collected and filtered by a millipore filter membrane with filtering particle diameter of 0.22 mu m, and the filter liquor is used as a test solution; secondly, a menbutone reference substance is weighed precisely, and after dissolved by acetonitrile and diluted by moving phase, the reference substance has a concentration of 9 to 11 mu g / mL, and a reference substance solution is obtained; thirdly, HPLC (High-Performance Liquid Chromatography) is adopted for testing the test solution and the reference substance solution, the chromatogram is recorded, and the menbutone residue in the tissue is calculated by peak area according to external standard method. The method is good in specificity, high in sensitivity and good in repeatability, can quickly detect the menbutone residue in the pig tissue, and is a reliable method for controlling the quantity of the menbutone residue in animal-origin food.

The invention discloses a preparation method of multiple acellular materials modified with nucleus pulposus cells for porcine small intestinal submucosa (SIS). The method specially comprises the following steps: stripping the porcine SIS tissues; carrying out treatment on the stripped porcine SIS tissues through a normal saline buffer solution containing a protease inhibitor, an organic solvent solution, a PBS (Phosphate Buffered Saline) buffer solution containing Triton X, a PBS buffer solution containing SDS (Sodium Dodecyl Sulfate) and a PBS buffer solution containing DNA enzyme to obtain acellular SIS materials; performing ball milling and pulverization on the acellular SIS materials to obtain micro-particles with diameter of 200mu m; carrying out co-culture on the micro-particles andseparated nucleus pulposus cells and then carrying out decellularization treatment again so as to obtain injectable multiple acellular nucleus pulposus repairing materials. According to the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS disclosed by the invention, the completeness of original ECM (Extra Cellular Matrix) can be preserved while allogeneic cells or heterologous cells with immunoreactivity are removed at the same time, and the preparation method of the multiple acellular materials modified with the nucleus pulposus cells for the porcine SIS has an good extracellular micro-environment, bio-factors, biomechanical properties and the like, and can simulate a growth environment of nucleus pulposus cells in normalphysiological conditions to a maximum limit.