PCR primer pair for identifying or assisting identification of animal tissue / or organ and application thereof

Abstract

The invention discloses a PCR primer pair for identifying or assisting identification of an animal tissue / or an organ and application thereof. The primer pair provided by the invention is at least one from five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ, wherein the PCR primer pair for identifying or assisting identification of pig tissue / or organ is always included. The five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ respectively consist of two DNA single chains shown as a sequence 1 and a sequence 2, two DNA single chains shown as a sequence 3 and a sequence 4, two DNA single chains shown as a sequence 5 and a sequence 6, two DNA single chains shown as a sequence 7 and a sequence 8, and two DNA single chains shown as a sequence 9 and a sequence 10 in the sequence table. An annealing temperature of 62 DEG C is employed for the five primer pairs to complete all PCR identifications at once and at a same temperature; besides, the PCR primer pair has strong singularity and a short detection time.

Description

technical field [0001] The present invention relates to a pair of PCR primers for identifying or assisting identification of animal tissues and / or organs and applications thereof, in particular to the tissue and / or identification of at least one animal among the five types of cattle, sheep, pigs, chickens and ducks or PCR primer pairs for organs. Background technique [0002] At present, in the field of production and sales of raw meat and meat products in China, some unscrupulous traders use adulteration and other means to deceive consumers in order to make huge profits. For example, pork is used as mutton, pork as beef, and chicken or duck as other meat with higher prices. Therefore, a set of methods for quickly and accurately identifying meat types and being able to adapt to the identification of mixed meat products will undoubtedly provide strong technical support for law enforcement officers. [0003] The traditional identification methods of meat products are mainly ...

AI Technical Summary

Problems solved by technology

This type of identification technology is greatly affected by the structure of the protein (the part that plays a major role is the antigenic determinant), and the problems that often occur in practical applications include: 1. The thermal processing process will change the structure of the protein (the antigenic determinant). Detect heat-processed meat; 2. Cross-reaction may occur in the detection of mixed meat, because the structure of proteins of different species may have a certain degree of similarity, and the sensitivity of the test depends on the specificity of the prepared antibody

At present, there are also reports on the use of PCR methods to identify beef, sheep, pigs, chickens, ducks, etc., but generally focus on a single meat species, and the system is poor; even if the method is integrated, in the process of PCR identification, different meat species The identity of the annealing temperature is poor, and multiple annealing temperatures and different PCR reactions are required to judge

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