A kind of physical method to effectively avoid the self-fertilization of Clam shell

A technology of self-fertilization and physical method, which is applied in the field of physics to effectively avoid self-fertilization of clam shells, can solve the problems of degeneration and provide technical support that has not been reported, and achieve the effect of preventing germplasm degradation and avoiding fertilization.

Active Publication Date: 2019-03-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low temperature is used to suppress the fertilization activity of sperm, and physical filtration is used to remove sperm to obtain unfertilized eggs, so as to avoid fertilization of sperm eggs from the same individual, and to provide technical support for the cultivation of giant clam artificial seedlings and the prevention of germplasm degradation. to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 The avoidance and prevention of self-fertilization of giant clam Tridacna maxima:

[0012] At the beginning of 2016, we carried out the research on the artificial breeding technology of giant clam (Tridacna maxima) at the Hainan Sanya Comprehensive Experimental Station of the Chinese Academy of Sciences. The artificial induced spawning method stimulated the production of 2 seedlings. In order to avoid self-fertilization and improve the breeding efficiency of seedlings, the specific operations are as follows:

[0013] 1. Prepare clean sand-filtered seawater in advance, and make 30L each of normal temperature seawater (about 28°C), medium temperature (transitional) seawater (24°C), and low temperature seawater (20°C) for use.

[0014] 2. Collect sperm under normal temperature conditions. Assign ID numbers to all the induced laboring clams, and place them in normal temperature sperm discharge barrels (for sperm collection). After 5-hydroxytryptamine injection, sp...

Embodiment 2

[0019] Example 2: Avoidance and prevention of self-fertilization of Tridac oyster Hippopus hippopus:

[0020] At the beginning of 2016, four 200L capacity plastic barrels were used to carry out the artificial breeding of giant oysters (Hippopus hippopus). In order to avoid self-fertilization and improve seed breeding efficiency, the specific operations are as follows:

[0021] 1. Prepare clean sand-filtered seawater in advance, and make 30L each of normal temperature seawater (about 28°C), medium temperature (transition) seawater (24°C), and low temperature seawater (20°C) for use.

[0022] 2. Collect sperm under normal temperature conditions. Assign ID numbers to all induced oysters, and place them in normal temperature sperm discharge barrels (for sperm collection). After 5-HT injection, sperm discharge begins 5 minutes later, and the sperm discharge lasts for about 2-3 minutes before being removed. Clam the oysters and filter the semen through a 500-mesh silk screen to fi...

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Abstract

The invention discloses a physical method capable of effectively avoiding self-fertilization of Tridacna sp. The physical method includes: allocating ID numbers to all the induced Tridacna sp, arranging the Tridacna sp into sperm discharging barrels holding normal temperature sea water, allowing the Tridacna sp to discharge sperms for 2-3 minutes, removing the Tridacna sp out from the sperm discharging barrels, using a 500 mesh bolting cloth to filter tissue blocks to obtain filtered liquid, wherein the filtered liquid is just sperm liquid, and the mark number of the sperm liquid is male+ the ID number; at meanwhile, arranging the removed Tridacna sp in intermediate barrels holding middle-temperature sea water for 1 minute, taking out the Tridacna sp and arranging the Tridacna sp in a ovum discharging barrels holding low-temperature sea water, allowing the Tridacna sp to continuously discharge ovum in the ovum discharging barrels for 15-20 minutes, and taking out the Tridacna sp from the ovum discharging barrels after ovum discharging to obtain ovum liquid, using a 300 mesh bolting cloth screen to filter to filter the ovum liquid to collect the ovum on the bolting cloth screen, marking the ovum as female + the ID number, and putting the collected ovum to barrels holding normal temperature sea water according to the numbers to recover for fertilization.

Description

technical field [0001] The invention belongs to the fields of giant clam shellfish breeding and ecological resource restoration in the marine aquatic industry, and in particular relates to a physical method for effectively avoiding giant clam shellfish self-fertilization. Background technique: [0002] Tridacna shellfish is an important marine economic shellfish. Its meat is sweet and crisp, and it has been reported to have the effect of nourishing yin and nourishing yang. At the same time, its shell can be carved and processed into valuable handicrafts. There used to be a large number of distributions in the South my country Sea. According to data, there are mainly species of giant clams, saffron clams, scaled clams, and oysters in the South my country Sea. At present, the library-type giant clam is nearly extinct in my country, and other species of giant clam are also facing unfavorable situations such as illegal harvesting and digging, as well as changes in the ecological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54
CPCY02A40/81
Inventor 肖述张跃环喻子牛马海涛向志明
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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