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A kind of yam polysaccharide polylactic acid glycolic acid nanoparticles and preparation method and application thereof

A technology of poly(lactic-co-glycolic acid) and lactic-co-glycolic acid, which is applied in the field of yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles and its preparation, can solve the problems of low bioavailability and poor effect, and achieve stable drug properties and encapsulation efficiency. The effect of high and strong sustained release

Active Publication Date: 2019-02-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a kind of yam polysaccharide polylactic-glycolic acid nanoparticle, to solve the problems such as low bioavailability and poor effect in the prior art

Method used

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  • A kind of yam polysaccharide polylactic acid glycolic acid nanoparticles and preparation method and application thereof
  • A kind of yam polysaccharide polylactic acid glycolic acid nanoparticles and preparation method and application thereof
  • A kind of yam polysaccharide polylactic acid glycolic acid nanoparticles and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] The preparation of embodiment 1 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticle

[0040] Accurately weigh a certain amount of yam polysaccharide in a small beaker, and dissolve it with ultrasound to form an inner water phase with a concentration of 10 mg / mL; accurately weigh a certain amount of polylactic acid glycolic acid, add acetone, and ultrasonically dissolve it to form an oil phase with a concentration of 20 mg / mL; Accurately weigh a certain amount of poloxamer 188, dissolve it in a certain amount of deionized water to form an external aqueous phase, and its concentration is 0.7%. Slowly add the inner water phase into the oil phase, and conduct ultrasound with a power of 120W, ultrasonic 2s, intermittent 3s, and a total time of 2min to obtain stable water-in-oil colostrum, slowly add the colostrum to the outer water phase, and 150W, ultrasonic 2s, intermittent 3s, total time 2min, sonicate to obtain a stable water-in-oil-in-water type double...

Embodiment 2

[0052] The characterization of embodiment 2 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticles

[0053] (1) Particle size potential detection and transmission electron microscope observation

[0054] Get the yam polysaccharide polylactic-glycolic acid nanoparticles (CYPP) and blank polylactic-glycolic acid nanoparticles (BP) prepared in Example 1 respectively (its preparation method is the same as in Example 1, the difference is that when preparing colostrum , using an equal volume of deionized water as the inner water phase to replace the prepared nanoparticles of the yam polysaccharide solution), using a Malvern particle size analyzer to analyze the particle size and potential of the two nanoparticles, the results are shown in Table 3.

[0055] The surface morphology was analyzed using transmission electron microscopy and scanning electron microscopy, the results are shown in figure 1 .

[0056] table 3

[0057]

[0058]

[0059] It can be seen f...

Embodiment 3

[0064] Embodiment 3 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticle in vitro immune enhancement activity comparison

[0065] Taking yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles (CYPP) prepared according to the optimal preparation conditions as the research object, and using yam polysaccharide (CYP) and blank poly(lactic-co-glycolic acid) (BP) as controls, to explore its effect on mouse lymphocyte proliferation in vitro Impact.

[0066] (1) Preparation of spleen lymphocytes

[0067] Mice were sacrificed by cervical dislocation and soaked in 75% ethanol for 3-5 minutes. In the ultra-clean bench, place the mouse in a plate with the left side facing up, open the skin and abdominal cavity at the last rib, carefully remove the spleen, and move the spleen into a 200-mesh steel sieve. Crush the spleen with scissors and tweezers, add a little PBS, grind the spleen gently with a glass grinding rod, add PBS while grinding (just add about 5 mL), a...

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Abstract

The invention discloses Chinese yam polysaccharide polylactic acid glycolic acid nanoparticles which are prepared from acetone, polylactic acid glycolic acid, Chinese yam polysaccharide and poloxamer 188 through a compound emulsion method. The invention further discloses a preparation method of the Chinese yam polysaccharide polylactic acid glycolic acid nanoparticles and application thereof in preparing immunity enhancement drugs. Compared with the prior art, the nanoparticles have the advantages that the nanoparticles are high in suspension encapsulation rate, stable in drug property and capable of making drug to be slowly released in animal bodies and effectively prolonging efficacy, have immunity enhancement effect and are of important significance in preventing and treating animal epidemic diseases. In addition, the nanoparticles serve as a novel Chinese veterinary drug and are free of side effect.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a yam polysaccharide polylactic-glycolic acid nanoparticle and a preparation method and application thereof. Background technique [0002] Yam is one of the medicinal and edible plants. Traditional Chinese medicine believes that yam has the effects of nourishing and strengthening, nourishing the spleen and stomach, promoting fluid and benefiting the lungs, tonifying the kidney and astringent essence, and curing thirst. Modern research has found that yam not only has a variety of nutrients, but also has medicinal values ​​such as flat nature, sweet taste, invigorating the spleen and stopping diarrhea, nourishing the lungs and kidneys. Yam contains active ingredients such as polysaccharides, allantoin, saponins, and glycoproteins, and polysaccharides, as active ingredients of plants and edible fungi, have become a hot spot in current pharmaceutical research. Yam polysaccharides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K36/8945A61K31/715A61P37/04
CPCA61K9/5146A61K31/715A61K36/8945
Inventor 王德云罗莉郑思思胡元亮刘家国武毅
Owner NANJING AGRICULTURAL UNIVERSITY
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