Chinese yam polysaccharide polylactic acid glycolic acid nanoparticles and preparation method and application thereof
The technology of poly(lactic acid glycolic acid and lactic acid glycolic acid) is applied in the field of yam polysaccharide poly(lactic acid glycolic acid) nanoparticles and their preparation, which can solve the problems of poor effect and low bioavailability, and achieve stable drug properties and strong sustained release effect. , the effect of high encapsulation rate
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Embodiment 1
[0039] The preparation of embodiment 1 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticle
[0040] Accurately weigh a certain amount of yam polysaccharide in a small beaker, and dissolve it with ultrasound to form an inner water phase with a concentration of 10 mg / mL; accurately weigh a certain amount of polylactic acid glycolic acid, add acetone, and ultrasonically dissolve it to form an oil phase with a concentration of 20 mg / mL; Accurately weigh a certain amount of poloxamer 188, dissolve it in a certain amount of deionized water to form an external aqueous phase, and its concentration is 0.7%. Slowly add the inner water phase into the oil phase, and conduct ultrasound with a power of 120W, ultrasonic 2s, intermittent 3s, and a total time of 2min to obtain stable water-in-oil colostrum, slowly add the colostrum to the outer water phase, and 150W, ultrasonic 2s, intermittent 3s, total time 2min, sonicate to obtain a stable water-in-oil-in-water type double...
Embodiment 2
[0052] The characterization of embodiment 2 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticles
[0053] (1) Particle size potential detection and transmission electron microscope observation
[0054] Get the yam polysaccharide polylactic-glycolic acid nanoparticles (CYPP) and blank polylactic-glycolic acid nanoparticles (BP) prepared in Example 1 respectively (its preparation method is the same as in Example 1, the difference is that when preparing colostrum , using an equal volume of deionized water as the inner water phase to replace the prepared nanoparticles of the yam polysaccharide solution), using a Malvern particle size analyzer to analyze the particle size and potential of the two nanoparticles, the results are shown in Table 3.
[0055] The surface morphology was analyzed using transmission electron microscopy and scanning electron microscopy, the results are shown in figure 1 .
[0056] table 3
[0057]
[0058]
[0059] It can be seen f...
Embodiment 3
[0064] Embodiment 3 Chinese yam polysaccharide polylactic acid glycolic acid nanoparticle in vitro immune enhancement activity comparison
[0065] Taking yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles (CYPP) prepared according to the optimal preparation conditions as the research object, and using yam polysaccharide (CYP) and blank poly(lactic-co-glycolic acid) (BP) as controls, to explore its effect on the proliferation of mouse lymphocytes in vitro Impact.
[0066] (1) Preparation of spleen lymphocytes
[0067] Mice were sacrificed by cervical dislocation and soaked in 75% ethanol for 3-5 minutes. In the ultra-clean bench, place the mouse in a plate with the left side facing up, open the skin and abdominal cavity at the last rib, carefully remove the spleen, and move the spleen into a 200-mesh steel sieve. Break the spleen with scissors and tweezers, add a little PBS, grind the spleen gently with a glass grinding rod, add PBS while grinding (just add about ...
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