A kind of breeding method of the cytoplasmic male sterile line of Brassica thaliana

Abstract

The invention discloses a method of breeding a cytoplasmic sterile line of Brassica juncea var. integlifolia, comprising the steps of selecting a cytoplasmic sterile line of Brassica juncea var. tumida JL154-5A (Brassica juncea) as a female parent, selecting a good selfing line of Brassica juncea var. integlifolia as a transfer-breeding male parent, hybridizing conventionally to obtain F1, backcrossing typical single offspring plants with the male parent continuously for 7 generations to obtain a cytoplasmic sterile line and maintainer line of Brassica juncea var. integlifolia that have stable sterile line character and good economic character. The male sterile line of Brassica juncea var. tumida is used as a sterile source, good germplasm of Brassica juncea var. tumida is used as the male parent, hybridizing and continuous backcrossing are carried out, the cytoplasmic sterile line and maintainer line of Brassica juncea var. integlifolia that have stable sterile character and good economic character are bred first at home and abroad, the hybrid bred germplasms of Brassica juncea vegetables (Brassica juncea var. tumida) are enriched, and the method is original at home and aboard; 'triple-line' mating of the Brassica juncea var. tumida is implemented, a new Brassica juncea var. tumida hybrid cultivar that is high yield, high quality and high disease resistance (tolerance) is bred, utilization of hybrid advantages is achieved, and the method has a promising market prospect.

Description

technical field [0001] The invention relates to the breeding technology of brassica mustard vegetables, in particular to a method for selecting a cytoplasmic male sterile line of mustard mustard. Background technique [0002] Brassica juncea var. latipa is a variety of Brassica juncea var. latipa leaf mustard vegetable, commonly known as bamboo shoots, green vegetables, and latipa, because of its wide and thick petioles and middle ribs, crisp texture Tender, with varying degrees of spicy taste, especially delicious when boiled in soup or shabu-shabu. It has the effects of removing heat, resisting rheumatism, benefiting the five internal organs, nourishing the sun and promoting blood circulation. It is very popular all over the world. It is a world-famous Sichuan sour The main raw material of (pickled) vegetables is also a characteristic fresh vegetable commonly used by citizens. At present, it is widely cultivated in various provinces and cities in southern my country, with an...

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports on the breeding and research of the cytoplasmic male sterile line of Brassica thaliana at home and abroad.

In particular, in terms of large-scale production of Paleopsis mustard, for a long time, no matter in Sichuan, Hunan, Guizhou and other provinces, as well as in Chongqing, local varieties with better performance have been directly used. The phenomenon of "selfing recession" is very common, and the direct result is that the growth of raw material crops is not uniform, the yield is not high, the quality is poor, and the resistance to disease (reversal) is weak, etc., which seriously restricts the rapid and healthy development of this industry.

Images