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Rice fertility regulation gene GMS3 as well as mutant and application thereof

A mutant, rice technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of geographical limitation of seed production, high seed production risk, fertility affected by light and temperature environment, etc., and achieve the effect of stable sterile traits

Pending Publication Date: 2022-05-27
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fertility of this type of sterile line is affected by the light and temperature environment, it also leads to high risk of seed production, and the area of ​​seed production is limited

Method used

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  • Rice fertility regulation gene GMS3 as well as mutant and application thereof
  • Rice fertility regulation gene GMS3 as well as mutant and application thereof
  • Rice fertility regulation gene GMS3 as well as mutant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Screening of rice male sterile mutant gms3

[0089] 10 kilograms of dry seeds of indica rice 93-11 were sent to the Hunan Academy of Agricultural Sciences for irradiation with cobalt 60, and the total radiation dose was 250 roentgens (Gy) to obtain M 0 It was planted in the Lingao Xinying base, and harvested separately after maturity, with a total of M 1 Substitute about 6000 copies of materials. The M1 generation material with more than 100 seeds was planted into a line, a total of 3500 copies, and 50 individual plants were planted in each line. Various types of mutants such as plant type, panicle type, fertility and yield were screened at tillering stage, booting stage, heading stage, flowering stage and grain-filling stage, and were harvested and stored. One of the mutants, 1199, exhibited male sterility and was named gms3.

Embodiment 2

[0090] Example 2 Phenotypic analysis of rice male sterile mutant gms3

[0091] Compared with wild type, gms3 mutant plants ( figure 1 ) and spikelets ( figure 2 ) in normal form, with a slightly late flowering. The floret morphology of the mutant was observed under a stereomicroscope, and it was found that the anther of gms3 was thinner and lighter than the wild type ( image 3 ). With iodine-potassium iodide solution (0.6% KI, 0.3% I 2 , w / w) solution stained the pollen, the mutant pollen grains were small, few, irregular and could not be stained ( Figure 4 ), while wild-type pollen grains were large, round and stained blue-black ( Figure 5 ). The wild-type plants of the same family were normally seed-bearing after bagging, but the gms3 mutant was not seed-bearing. However, using rice variety 93-11 as the male parent to pollinate the gms3 mutant can produce fruit. This indicates that the mutant is a male sterile mutant.

Embodiment 3

[0092] Example 3 Genetic analysis of rice male sterile mutant gms3

[0093] In the M4 generation, 1321 isolates of gms3 were planted, of which 996 were normal in fertility and 325 were sterile. The segregation ratio of fertile and sterile plants was in line with 3:1 (χ 2 =0.08, P2 =1.06, P<0.05). The above results indicate that the sterility trait of gms3 is controlled by a recessive single gene.

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Abstract

The invention provides a rice fertility regulation gene GMS3 as well as a mutant and application thereof. The invention provides a rice gene GMS3 with functions of regulating and controlling rice male germ cell development and pollen fertility, the CDS sequence of the rice gene GMS3 is as shown in SEQ ID NO: 2, and the amino acid sequence of the rice gene GMS3 is as shown in SEQ ID NO: 3. The invention provides a radiation mutagenesis mutant of a GMS3 gene and a CRISPR (clustered regularly interspaced short palindromic repeats) knockout mutant, and provides a molecular marker identification method of the mutants. The rice gene GMS3 provided by the invention can be used for sterile seed production and production of rice hybrids, and has huge application value and economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a plant fertility regulation gene GMS3, a GMS3-encoded protein, a radiation mutagenesis and gene knockout mutant of GMS3, and the application of GMS3 gene, protein and mutant in cross-breeding. Background technique [0002] Hybrid rice is the progeny obtained after the hybridization of the parental parent. Therefore, the application and promotion of hybrid rice is an important way to improve rice yield. [0003] Male sterile line is the key node of hybrid rice breeding technology. Male sterile lines refer to plant lines in which male gametes develop abnormally and lose fertility, while female gametes develop normally. It can only receive pollen from the male parent as a female parent, and cannot produce fruit by selfing. At present, there are two types of male sterile lines used in hybrid rice production: nuclear-cytoplasmic interaction type and photo-thermosensitive ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8289
Inventor 李京琳龙湍唐杰吴春瑜李新鹏安保光曾翔吴永忠黄培劲
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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