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Rice fertility regulating gene and its mutant and application thereof

A mutant, rice technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of fertility affected by light and temperature environment, high risk of seed production, and geographical restrictions of seed production.

Active Publication Date: 2019-11-29
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fertility of this type of sterile line is affected by the light and temperature environment, it also leads to high risk of seed production, and the area of ​​seed production is limited

Method used

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  • Rice fertility regulating gene and its mutant and application thereof
  • Rice fertility regulating gene and its mutant and application thereof
  • Rice fertility regulating gene and its mutant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1 Screening of rice male sterile mutant gms1

[0093] In June 2013, 10 kg of 93-11 seeds were irradiated with cobalt 60 to obtain M 0 generation. The irradiated seeds were planted in the experimental field of Lingao County, Hainan Province, and harvested by individual plants after maturity. A total of M 1 There are about 6,500 substitute materials. In the spring of 2014, 3617 M with more seeds were selected 1The generation materials were planted into strains, and 50 individual plants were planted in each strain. Various types of mutants such as plant type, panicle type, fertility, yield, etc. were screened at the tillering stage, booting stage, heading stage, flowering stage, and grain filling stage, and harvested and stored. One of the mutants exhibited male sterility and was named gms1.

Embodiment 2

[0094] Example 2 Phenotype analysis of rice male sterile mutant gms1

[0095] Compared with the wild type, gms1 mutant plants ( figure 1 ) and Xiaosui ( figure 2 ) is normal in shape, and the flowering period is a little later. The size of palea and lemma, the opening size of florets, and the opening time were not significantly different from those of the wild type ( image 3 ). Observing the shape of the mutant florets under a stereomicroscope, it was found that the ovary, style, and stigma were slightly larger than those of the wild type ( Figure 4 ), but the anthers are thinner and lighter than the wild type ( Figure 5 ). With iodine-potassium iodide solution (0.6% KI, 0.3% I 2 , w / w) solution dyes pollen, such as Figure 6 The wild-type pollen grains shown are large, round and stained blue-black, while the mutant pollen grains are shrunken and cannot be stained. The wild-type plants of the same family set fruit normally after bagging selfing, but the 3013 mutant...

Embodiment 3

[0096] Example 3 Genetic Analysis of Rice Male Sterility Mutant gms1

[0097] In the segregation population of gms1 planted in the M4 generation, 436 plants were planted, of which 330 plants had normal fertility and 106 plants were sterile, and the segregation ratio between fertile and sterile plants was 3:1 ( 2 =0.08, P2 = 1.06, P<0.05). The above results indicated that the sterility trait of gms1 was controlled by a recessive single gene.

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Abstract

The invention provides a rice fertility regulating gene and its mutant and an application thereof. The invention provides a rice gene GMS1, which has the functions of regulating rice male germ cell development and pollen fertility, a CDS sequence is shown as SEQ ID NO: 2, and an amino acid sequence is shown as SEQ ID NO: 3. The invention provides a radiation mutagenesis mutant and a CRISPR knockout mutant of the GMS1 gene, and provides a molecular marker identification method for the mutant. The rice gene GMS1 provided by the present invention can be used for sterile breeding and production ofrice hybrids, and has great application value and economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant fertility regulation gene GMS1, GMS1 encoded protein, radiation mutagenesis and gene knockout mutant of GMS1, and application of GMS1 gene, protein and mutant in hybrid breeding. Background technique [0002] Rice is one of the most important food crops in the world. With the growth of population and the improvement of quality of life, it is estimated that by 2050, the annual output of rice will increase by 1-2 times to meet the needs of human development. Hybrid rice is the generation of offspring obtained by crossing parents. Its yield is often 15% higher than that of conventional rice parents, and its resistance and adaptability are far better than the parents. Therefore, the application and popularization of hybrid rice is an important way to increase rice yield. [0003] The male sterile line is a key node in hybrid rice breeding technology. Male sterile l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/18C12N15/82A01H5/02A01H6/46C12Q1/6895C12N15/11
CPCC12N9/18C12N15/8213C12N15/8289C12N2810/10C12Q1/6895C12Q2600/13C12Q2600/156A01H5/00A01H5/02A01H6/46C12N15/11C12N15/113C12N15/82
Inventor 黄培劲龙湍唐杰刘昊李佳林李新鹏安保光曾翔吴永忠
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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