Method of breeding cytoplasmic sterile line of Brassica juncea var. integlifolia

A male sterile line and mustard cytoplasmic technology, applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the unreported and messy breeding and research of broad handle mustard cytoplasmic male sterile line , weak disease resistance and other issues, to achieve the effect of realizing heterosis utilization and broad market prospects

Inactive Publication Date: 2016-12-07
重庆市渝东南农业科学院
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Problems solved by technology

[0003] At present, there are no reports on the breeding and research of the cytoplasmic male sterile line of Brassica thaliana at home and abroad.
In particular, in terms of large-scale production of Paleopsis mustard, for a long time, no matter in Sichuan, Hunan, Guizhou and other provinces, as well as in Chongqing, local varieties with better performance have been directly used. The phenomenon of "selfing recession" is very common, and the direct result is that the growth of raw material crops is not uniform, the yield is not high, the quality is poor, and the resistance to disease (reversal) is weak, etc., which seriously restricts the rapid and healthy development of this industry.

Method used

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  • Method of breeding cytoplasmic sterile line of Brassica juncea var. integlifolia
  • Method of breeding cytoplasmic sterile line of Brassica juncea var. integlifolia

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Embodiment Construction

[0013] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples. It should be understood that the specific implementation examples described here are only used to explain the present invention, and are not intended to limit the present invention.

[0014] like figure 1 As shown, the embodiments of the present invention provide a method for breeding a cytoplasmic male sterile line of Brassica thaliana, comprising the steps of:

[0015] S1. Using the stem tumor mustard ( mustard mustard ) cytoplasmic male sterile line "JL154-5A" bred by Fan Yonghong, Chongqing Yudongnan Academy of Agricultural Sciences, etc. as the female parent, in March 2011, systematically identified and confirmed "JL154- On the basis of the stable 5A” sterility traits, the excellent inbred lines DJ, K6, HK-1, HK-2, K46, and BK of different traits, different ecological types, and different maturi...

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Abstract

The invention discloses a method of breeding a cytoplasmic sterile line of Brassica juncea var. integlifolia, comprising the steps of selecting a cytoplasmic sterile line of Brassica juncea var. tumida JL154-5A (Brassica juncea) as a female parent, selecting a good selfing line of Brassica juncea var. integlifolia as a transfer-breeding male parent, hybridizing conventionally to obtain F1, backcrossing typical single offspring plants with the male parent continuously for 7 generations to obtain a cytoplasmic sterile line and maintainer line of Brassica juncea var. integlifolia that have stable sterile line character and good economic character. The male sterile line of Brassica juncea var. tumida is used as a sterile source, good germplasm of Brassica juncea var. tumida is used as the male parent, hybridizing and continuous backcrossing are carried out, the cytoplasmic sterile line and maintainer line of Brassica juncea var. integlifolia that have stable sterile character and good economic character are bred first at home and abroad, the hybrid bred germplasms of Brassica juncea vegetables (Brassica juncea var. tumida) are enriched, and the method is original at home and aboard; 'triple-line' mating of the Brassica juncea var. tumida is implemented, a new Brassica juncea var. tumida hybrid cultivar that is high yield, high quality and high disease resistance (tolerance) is bred, utilization of hybrid advantages is achieved, and the method has a promising market prospect.

Description

technical field [0001] The invention relates to the breeding technology of brassica mustard vegetables, in particular to a method for selecting a cytoplasmic male sterile line of mustard mustard. Background technique [0002] Brassica juncea var. latipa is a variety of Brassica juncea var. latipa leaf mustard vegetable, commonly known as bamboo shoots, green vegetables, and latipa, because of its wide and thick petioles and middle ribs, crisp texture Tender, with varying degrees of spicy taste, especially delicious when boiled in soup or shabu-shabu. It has the effects of removing heat, resisting rheumatism, benefiting the five internal organs, nourishing the sun and promoting blood circulation. It is very popular all over the world. It is a world-famous Sichuan sour The main raw material of (pickled) vegetables is also a characteristic fresh vegetable commonly used by citizens. At present, it is widely cultivated in various provinces and cities in southern my country, with an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 冷容范永红李娟杨仕伟刘雪娇
Owner 重庆市渝东南农业科学院
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