34results about How to "Unaffected by environmental conditions" patented technology

The invention discloses a fragrant immobilized granular activation-free chlorine dioxide air purifying agent and belongs to the field of environmental purification. The air purifying agent includes following components: a solidifying agent, a chlorine dioxide generating substance, spices, a coloring agent, a slow-releasing agent, a filling agent and the like. A preparation method includes the steps of material preparation, grinding, drying, sieving, mixing and moulding. The air purifying agent can effectively kill bacteria, virus, chemical toxins and residual pesticides and the like harmful substances. The air purifying agent is convenient to use, is simple in operation, is significant in effects, is long-acting, safe and long-shelf-life, and has wide application prospect in the fields of household environments, medical care and health care, hygiene and epidemic prevention and the like.

The present invention discloses one kind of primer series for identifying cucumber powdery mildew and its identification method. The primer series consists of upstream primer 5'-CAG TAA ATG AAA GAA AAG AAG-3' and downstream primer 5'-ATA CAT AGC CAT ACA AAA AT-3'. The identification method of cucumber powdery mildew with the primer series includes the following steps: extracting cucumber genomeDNA; PCR proliferation; gel electrophoresis analysis of the PCR proliferation product; and identifying the resistance of cucumber powdery mildew according to the relative position of the identified sample on gel strip. The said method has its result possess coincidence rate with field detection result up to 94 %, and is fast, accurate and not affected by environment condition, so that it has high application value in screening cucumber powdery mildew resistance.

The invention discloses a method for processing an iridium alloy bar or plate. The method comprises the following steps of: wrapping and welding an iridium alloy to be processed by using a hot-rolled metal molybdenum plate as a wrapping material at first; placing the iridium alloy wrapped by the hot-rolled metal molybdenum plate into a hydrogen resistance furnace and heating; processing the heated iridium alloy wrapped by the hot-rolled metal molybdenum plate to obtain a bar or plate; annealing the obtained bar or plate; and finally immersing the annealed bar or plate into chloroazotic acid and eliminating the wrapped hot-rolled metal molybdenum plate so as to obtain the iridium alloy bar or plate. The method is simple and easy to implement, and no special equipment is needed; and by using the advantage of the metal molybdenum and adopting a wrapping technology, the iridium alloy is wrapped into the hot-rolled metal molybdenum plate, so the iridium alloy cannot be influenced by the environmental condition during processing, and the iridium alloy can be processed successfully.

The invention discloses a paddy rice male fertility regulating gene OsSTRL2 and application thereof and belongs to the technical field of plant biology. The nucleotide sequence of the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID NO. 1, and the amino acid sequence of protein encoded by the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID No. 2. The invention further provides a method for culturing male sterile plants. The paddy rice male fertility regulating gene OsSTRL2 and a male sterile line generated on the basis of the gene have the advantages that the male sterile line is stable in fertility, unaffected by environment conditions and capable of being recovered by wild transgenosis; the gene and the male sterile line provide necessary components for the building of novel cross breeding systems and are significant in production practice.

The invention belongs to the field of building materials, and provides an epoxy resin sports floor material. The epoxy resin sports floor material is prepared from the following raw materials in partsby weight: 30 to 40 parts of epoxy resin, 10 to 15 parts of butadiene styrene rubber, 1 to 5 parts of carboxyl-terminated liquid nitrile butadiene rubber, 5 to 10 parts of shale fibers, 5 to 10 partsof aluminum sulfate, 1 to 5 parts of butanol, 1 to 5 parts of petroleum ether, 1 to 5 parts of vinyl distearamide and 1 to 5 parts of sodium gluconate. A prepared product is high in stability and cannot be affected by environmental conditions; the shortcoming that a conventional coating epoxy floor product is sensitive to objective environmental conditions such as temperature and humidity in a curing process is overcome; the product is uniform in texture and proper in elasticity.

The present invention discloses one kind of primer series for identifying cucumber powdery mildew and its identification method. The primer series consists of upstream primer 5'-CAG TAA ATG AAA GAA AAG AAG-3' and downstream primer 5'-ATA CAT AGC CAT ACA AAA AT-3'. The identification method of cucumber powdery mildew with the primer series includes the following steps: extracting cucumber genome DNA; PCR proliferation; gel electrophoresis analysis of the PCR proliferation product; and identifying the resistance of cucumber powdery mildew according to the relative position of the identified sample on gel strip. The said method has its result possess coincidence rate with field detection result up to 94 %, and is fast, accurate and not affected by environment condition, so that it has high application value in screening cucumber powdery mildew resistance.

The invention relates to a method using sequence characterize amplified region (SCAR) signs to identify sugarcane brown rust disease resistance, which comprises extraction of genome deoxyribonucleic acid (DNA), detection system establishment of SCAR- polymerase chain reaction (PCR) signs and detection of SCAR-PCR products. The method using SCAR signs to identify sugarcane brown rust disease resistance has the advantages of being high in flexibility and small in using amount of DNA. Compared with general resistance identification, the method has the advantages of being convenient in operation,short in detection time, high in accuracy, easy in judgment, good in repeatability, free of effect of environmental conditions, and the like. The method provides a convenient and effective brown rustdisease resistance identification system for assessment of sugarcane germplasm resources and accreditation of new products and has significance on enhancing brown rust resistance sugarcane breading and protection on brown rust resistance sugarcane germplasm resources.

The invention is a primer sequence for identifying resistance to cucumber frost mildew and identifying method, where the primer sequence is composed of an upper primer with a nucleotide sequence described in sequence list SEQ ID No.1 and an lower primer with that in sequence list SEQ ID No.2; includes the steps: (1) extracting a cucumber gene group DNA; (2) making PCR amplification by using the above two nucleotide sequences; (3) making gel electrophoretic analysis on the product of PCR amplification; (4) according to the relative position of all the identified samples, identifying the resistance to cucumber frost mildew, and the fitting rate of the identified result to the field resistance to diseases reaches 94.7% and it has the advantages of rapidness, accuracy, no influence by the environmental conditions, etc, and has very great application value in screening the resistance to cucumber frost mildew.

The invention discloses a rice fertility gene SAW1 and its application. The nucleotide sequence of the gene SAW1 is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. Knocking out the gene SAW1 can abort rice anthers, and can be applied to the creation of sterile lines and the preparation of hybrid seeds. The male sterile line produced based on the mutation of gene SAW1 has stable fertility and is not affected by environmental conditions. It provides necessary elements for the construction of a new hybrid breeding system and has good application value and prospects.

The invention discloses gene segments linked with yellow and green cotyledons of cucumbers and application. The gene segment linked with yellow cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.1, and the gene segment linked with green cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.2. The gene segment linked with yellow cotyledons of cucumbers and the gene segment linked with green cotyledons of cucumbers are used for detecting the constructed F2-generation segregation population and cucumber germplasm resources, the identification shows that the coincidence rate of the detection result and the artificial phenotype reaches 96.77%, and the linkage inheritance distance of the genes related to the colors of cucumber cotyledons is 3.2cm. The invention has the advantages of rapidness, accuracy, no influence of environmental conditions and the like, and has a great application value for molecular marker-assisted breeding of cucumbers.

The invention discloses a paddy rice male fertility regulating gene OsSTRL2 and application thereof and belongs to the technical field of plant biology. The nucleotide sequence of the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID NO. 1, and the amino acid sequence of protein encoded by the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID No. 2. The invention further provides a method for culturing male sterile plants. The paddy rice male fertility regulating gene OsSTRL2 and a male sterile line generated on the basis of the gene have the advantages that the male sterile line is stable in fertility, unaffected by environment conditions and capable of being recovered by wild transgenosis; the gene and the male sterile line provide necessary components for the building of novel cross breeding systems and are significant in production practice.

The invention provides steel plate rust removal equipment. The steel plate rust removal equipment comprises a grinding and cleaning head, wherein the grinding and cleaning head is a disc, a replaceable steel wire brush is arranged on the disc, and the disc is connected with a driving motor; the grinding and cleaning head is connected with a long handle, a cable is connected to the rear end of thehandle, and a control switch is arranged on the handle; and a blowing nozzle is arranged in the middle of the grinding and cleaning head, fan blades are arranged inside the blowing nozzle, and the fanblades are connected with a motor shaft of the driving motor through a gear. The steel plate dust removal method comprises the steps that during rust removal, firstly, a rust remover is uniformly sprayed on a steel plate needing rust removal; then rust removing and grinding are carried out on the steel plate by using the rust removal equipment with the steel wire brush; and finally, antirust paint is sprayed on the steel plate.

The invention discloses a primer sequence for identifying resistance of cucumber against alternaria cucumerina and an identification method thereof. The primer sequence comprises nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table. The method for identifying the resistance of the cucumber against the alternaria cucumerina comprises the following steps of: (1) extracting genomic DNA of the cucumber; (2) performing polymerase chain reaction (PCR) amplification by taking the nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2 in the sequence table as primers; (3) performing gel electrophoresis analysis on the amplification product; and (4) identifying the resistance of the cucumber against the alternaria cucumerina according to the relative position of each identified sample on a gel strip. By evaluating the resistance of the constructed segregation population of generation F2 and cucumber germplasm resources against the alternaria cucumerina with the primer sequence of the invention, the coincidence rates for the result and artificial inoculation evaluation reach 95.04 percent and 94.74 percent respectively; the linkage genetic distance with the alternaria cucumerina resistance gene is 4.96cm; and the primer sequence has the advantages of high speed, accuracy and freedom from environmental condition and the like, and has great application value for screening the resistance of the resources in the cucumber breeding.

The invention discloses a method for processing an iridium alloy bar or plate. The method comprises the following steps of: wrapping and welding an iridium alloy to be processed by using a hot-rolled metal molybdenum plate as a wrapping material at first; placing the iridium alloy wrapped by the hot-rolled metal molybdenum plate into a hydrogen resistance furnace and heating; processing the heated iridium alloy wrapped by the hot-rolled metal molybdenum plate to obtain a bar or plate; annealing the obtained bar or plate; and finally immersing the annealed bar or plate into chloroazotic acid and eliminating the wrapped hot-rolled metal molybdenum plate so as to obtain the iridium alloy bar or plate. The method is simple and easy to implement, and no special equipment is needed; and by using the advantage of the metal molybdenum and adopting a wrapping technology, the iridium alloy is wrapped into the hot-rolled metal molybdenum plate, so the iridium alloy cannot be influenced by the environmental condition during processing, and the iridium alloy can be processed successfully.

The invention relates to the field of interaction of laser and matter in strong-field physics, in particular to an optical soliton generation device and method with an adjustable center frequency. The optical soliton generation device comprises a laser, an action structure and a receiver. The method includes the steps that S1, an interval d between periodical medium planes is selected, the size delta of a periodical medium is obtained according to the formula that d=2*delta, and a periodical micro-nano structure is formed; S2, short-period ultra-short laser pulse is introduced, and the laser pulse is transmitted in the medium; S3, the reflection electric field and the transmission electric field generated when the pulse is transmitted in the periodical micro-nano structure are recorded respectively; S4, the recorded data are processed and analyzed, and then soliton pulse is obtained. By changing the size of the medium in the periodical micro-nano structure, the transmission process of the pulse in the micro-nano structure is affected, the final transmission electric field is further affected, and solitons of different frequencies are obtained. The optical soliton generation method is simple, easy to implement, free of influences of environment conditions and good in stability and repeatability of the system, and well realizes the adjustable center frequency of the optical solitons.

The invention provides a rice fertility regulation gene, its mutant and application. The invention provides a rice gene GMS1 with functions of regulating rice male germ cell development and pollen fertility, its CDS sequence is shown in SEQ ID NO:2, and its amino acid sequence is shown in SEQ ID NO:3. The invention provides radiation mutagenesis mutants and CRISPR knockout mutants of the GMS1 gene, and a molecular marker identification method for the mutants. The rice gene GMS1 provided by the invention can be used for sterile seed production and production of rice hybrids, and has great application value and economic value.

The invention discloses a DNA molecule identification method of coenospecies of Xishi abalone and Haliotis discus hannai Ino, relating to the DNA molecule marker detection of an abalone, in particular to a method for carrying out molecule identification to the authenticity of the coenospecies of an interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino by utilizing a molecule marker technology (microsatellite technology). The invention provides a DNA molecule identification method of the coenospecies of the Xishi abalone and the Haliotis discus hannai Ino by using microsatellite molecular marker, which has the advantages of high polymorphism, stable heredity and not being affected by environment conditions, etc, and can identify the authenticity of the coenospecies of the interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino during the large scale interspecific hybridization species production process of the Xishi abalone and the Haliotis discus hannai Ino. The genome DNA of the coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino is extracted by adopting a phenol chloroform method and PCR amplification reaction is carried out by taking the extracted genome DNA as a template to obtain the amplification product; gel electrophoresis and dyeing are carried out to the amplification product, and the map is compared; and comparison is carried out according to the electrophoresis result and the provided standard map.

The invention relates to a marker gene for identifying blueberry heat-resistant varieties and application thereof, belonging to the field of plant biotechnology. Utilizing the marker gene, thioredoxin o-type (TRXo), was able to up-regulate expression to different degrees in different heat-tolerant blueberry varieties under high temperature stress. Primers were designed using the CDS sequence of the gene as a template, and the detection method was to artificially simulate the climate and heat stress at 45°C for 6 h, extract total RNA, reverse transcribe it into cDNA as a template, perform qRT‑PCR detection, and use the 2‑ΔΔCt method to calculate analyze. By analyzing the ratio of the expression of the gene under heat stress and the control, if the ratio is greater than or equal to 5, the blueberry variety can be identified as a heat-tolerant variety. Compared with the field natural identification method, the method is faster, more stable and more accurate, and is the basis for rapid screening of heat-resistant varieties for popularization and planting and breeding of new heat-resistant varieties.

The invention provides a rice fertility regulation gene GMS3 as well as a mutant and application thereof. The invention provides a rice gene GMS3 with functions of regulating and controlling rice male germ cell development and pollen fertility, the CDS sequence of the rice gene GMS3 is as shown in SEQ ID NO: 2, and the amino acid sequence of the rice gene GMS3 is as shown in SEQ ID NO: 3. The invention provides a radiation mutagenesis mutant of a GMS3 gene and a CRISPR (clustered regularly interspaced short palindromic repeats) knockout mutant, and provides a molecular marker identification method of the mutants. The rice gene GMS3 provided by the invention can be used for sterile seed production and production of rice hybrids, and has huge application value and economic value.