DNA molecular identification method of Haliotis sieboldii and Haliotis discus Hannai hybrid

Abstract

The invention discloses a DNA molecule identification method of coenospecies of Xishi abalone and Haliotis discus hannai Ino, relating to the DNA molecule marker detection of an abalone, in particular to a method for carrying out molecule identification to the authenticity of the coenospecies of an interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino by utilizing a molecule marker technology (microsatellite technology). The invention provides a DNA molecule identification method of the coenospecies of the Xishi abalone and the Haliotis discus hannai Ino by using microsatellite molecular marker, which has the advantages of high polymorphism, stable heredity and not being affected by environment conditions, etc, and can identify the authenticity of the coenospecies of the interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino during the large scale interspecific hybridization species production process of the Xishi abalone and the Haliotis discus hannai Ino. The genome DNA of the coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino is extracted by adopting a phenol chloroform method and PCR amplification reaction is carried out by taking the extracted genome DNA as a template to obtain the amplification product; gel electrophoresis and dyeing are carried out to the amplification product, and the map is compared; and comparison is carried out according to the electrophoresis result and the provided standard map.

Description

DNA Molecular Identification Method of Hybrids of Siberian Abalone and Panbao wrinkle technical field The present invention relates to the detection of DNA molecular markers of abalone, in particular to a method for molecular identification of the authenticity of the hybrids of the first generation of interspecific hybrids of Siberian abalone and wrinkled disc abalone using molecular marker technology-microsatellite (SSR) technology . Background technique Abalone is a kind of marine shellfish, known as the first of the eight seafood treasures, known as "soft gold", and has extremely high economic value. The main cultured abalone species in my country are Haliotis discushannai and Haliotis diversicolor. Among them, Haliotis discushannai is a temperate species, which is mainly distributed in the Liaodong Peninsula and Shandong Peninsula in my country, and belongs to the northern part of my country. The main cultured abalone species in coastal areas. Due to its high economic...

AI Technical Summary

Problems solved by technology

However, the technical requirements are strict, the procedure is complicated, the recognition is not intuitive, and the expression of isozymes is related to the tissue and developmental stage, so the polymorphism is low, the types of enzymes available are less, the repeatability is not good enough, and the requirements are high The laboratory conditions are not easy to analyze a large number of samples

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