A kind of rice fertility regulation gene and its mutant and application
A technology of mutants and rice, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of geographical restrictions on seed production, fertility affected by light and temperature environment, high risk of seed production, etc., and achieve the effect of stable sterility traits
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Embodiment 1
[0092] Example 1 Screening of rice male sterile mutant gms1
[0093] In June 2013, 10 kg of 93-11 seeds were irradiated with cobalt 60 to obtain M 0 generation. The irradiated seeds were planted in the experimental field of Lingao County, Hainan Province, and harvested by individual plants after maturity. A total of M 1 There are about 6,500 substitute materials. In the spring of 2014, 3617 M with more seeds were selected 1The generation materials were planted into strains, and 50 individual plants were planted in each strain. Various types of mutants such as plant type, panicle type, fertility, yield, etc. were screened at the tillering stage, booting stage, heading stage, flowering stage, and grain filling stage, and harvested and stored. One of the mutants exhibited male sterility and was named gms1.
Embodiment 2
[0094] Example 2 Phenotype analysis of rice male sterile mutant gms1
[0095] Compared with the wild type, gms1 mutant plants ( figure 1 ) and Xiaosui ( figure 2 ) is normal in shape, and the flowering period is a little later. The size of palea and lemma, the opening size of florets, and the opening time were not significantly different from those of the wild type ( image 3 ). Observing the shape of the mutant florets under a stereomicroscope, it was found that the ovary, style, and stigma were slightly larger than those of the wild type ( Figure 4 ), but the anthers are thinner and lighter than the wild type ( Figure 5 ). With iodine-potassium iodide solution (0.6% KI, 0.3% I 2 , w / w) solution dyes pollen, such as Figure 6 The wild-type pollen grains shown are large, round and stained blue-black, while the mutant pollen grains are shrunken and cannot be stained. The wild-type plants of the same family set fruit normally after bagging selfing, but the 3013 mutant...
Embodiment 3
[0096] Example 3 Genetic Analysis of Rice Male Sterility Mutant gms1
[0097] In the segregation population of gms1 planted in the M4 generation, 436 plants were planted, of which 330 plants had normal fertility and 106 plants were sterile, and the segregation ratio between fertile and sterile plants was 3:1 ( 2 =0.08, P2 = 1.06, P<0.05). The above results indicated that the sterility trait of gms1 was controlled by a recessive single gene.
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