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Rice male fertility regulation gene mutant as well as molecular marker and application thereof

A technology of male fertility and mutants, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of fertility affected by light and temperature environment, high risk of seed production, and geographical restrictions of seed production, achieving great application value, Effective method, high utilization rate of germplasm resources

Pending Publication Date: 2021-12-07
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fertility of this type of sterile line is affected by the light and temperature environment, it also leads to high risk of seed production, and the area of ​​seed production is limited

Method used

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  • Rice male fertility regulation gene mutant as well as molecular marker and application thereof
  • Rice male fertility regulation gene mutant as well as molecular marker and application thereof
  • Rice male fertility regulation gene mutant as well as molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Screening of rice male sterile mutant gms2

[0092] In June 2013, 10 kg of 93-11 seeds were irradiated with cobalt 60 to obtain M 0 generation. The irradiated seeds were planted in the experimental field of Lingao County, Hainan Province, and harvested by individual plants after maturity. A total of M 1 There are about 6,500 substitute materials. In the spring of 2014, 3617 M with more seeds were selected 1 The generation materials were planted into strains, and 50 individual plants were planted in each strain. Various types of mutants such as plant type, panicle type, fertility, yield, etc. were screened at the tillering stage, booting stage, heading stage, flowering stage, and grain filling stage, and harvested and stored. Among them, a sterile mutant was found in the line numbered 3148, named gms2.

Embodiment 2

[0093] Example 2 Phenotype analysis of rice male sterile mutant gms2

[0094] Compared with wild type, gms2 mutant plants ( figure 1) and Xiaosui ( figure 2 ) is normal in shape, and the flowering period is a little later. The size of palea and lemma, the opening size of florets, and the opening time were not significantly different from those of the wild type ( image 3 ). Observing the shape of the mutant florets under a stereomicroscope, it was found that the ovary, style, and stigma were slightly larger than those of the wild type ( Figure 4 ), but the anthers are thinner and lighter than the wild type ( Figure 5 ). With iodine-potassium iodide solution (0.6% KI, 0.3% I 2 , w / w) solution dyes pollen, such as Image 6 As shown, the wild-type pollen grains are large and round and are stained blue-black, while the mutant pollen grains are shrunken and cannot be stained. The wild-type plants of the same family set fruit normally after bagging selfing, but the gms2 m...

Embodiment 3

[0095] Example 3 Genetic Analysis of Rice Male Sterility Mutant gms2

[0096] In the M5 generation, 80 strains of the segregation population of gms2 were planted, of which 64 strains had normal fertility and 16 strains were sterile, and the segregation ratio between fertile and sterile strains was 3:1 (χ 2 =0.57, P>0.05). By backcrossing gms2 with 93-11, all F1 plants were fertile. In the F2 generation, 70 strains of gms2 segregation population were planted, of which 57 strains had normal fertility and 13 strains were sterile, and the segregation ratio between fertile and sterile strains was 3:1 (χ 2 =0.85, P>0.05). The above results indicated that the sterility trait of gms2 was controlled by a recessive single gene.

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Abstract

The invention relates to the technical field of biology, in particular to a rice male fertility regulation gene mutant as well as a molecular marker and application thereof. The invention provides a rice male fertility regulation protein GMS2 mutant that can enable rice pollen to be completely sterile and lead to rice male sterility. The GMS2 mutant has a genome nucleotide sequence as shown in SEQ ID NO: 6, a CDS sequence as shown in SEQ ID NO: 7, and an amino acid sequence as shown in SEQ ID NO: 8. The mutant can be used for cultivating a new genetic male sterility line, and a simple, rapid and effective method is provided for cultivating the rice genetic male sterility line. A genetic male sterility rice material according to the invention can be used for replacing artificial emasculation during rice hybridization, save labor, especially can be used for recurrent selection breeding that requires a large number of hybridizations, and plays an important role in expanding the germplasm base of rice.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant fertility-related protein GMS2 mutant, the mutant's coding gene, its molecular marker and its application in hybrid breeding. Background technique [0002] Rice is one of the most important food crops in the world. With the growth of population and the improvement of quality of life, it is estimated that by 2050, the annual output of rice will increase by 1-2 times to meet the needs of human development. Hybrid rice is the generation of offspring obtained by crossing parents. Its yield is often 15% higher than that of conventional rice parents, and its resistance and adaptability are far better than the parents. Therefore, the application and popularization of hybrid rice is an important way to increase rice yield. [0003] The male sterile line is a key node in hybrid rice breeding technology. Male sterile lines refer to plant strains with abnormal development of male gam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/02A01H6/46A01H1/02C12Q1/6895C12N15/11
CPCC07K14/415C12N15/8218C12N15/8289A01H1/02C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 龙湍唐杰李燕群李佳林刘昊曾翔李新鹏安保光吴永忠黄培劲
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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