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Gene segments linked with yellow and green cotyledons of cucumbers and application

A technology of gene fragments and cotyledons, which is applied in the field of gene fragments linked to the color of cucumber cotyledons, can solve the problems of few reports on the molecular mechanism of cucumber leaf color mutation traits, and achieve the effect of not being affected by environmental conditions.

Inactive Publication Date: 2012-09-19
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, there were many studies on the physiology and genetics of cucumber true leaf color mutation traits, but less research on cucumber cotyledon color, and few reports on the molecular mechanism of cucumber leaf color mutation traits.

Method used

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  • Gene segments linked with yellow and green cotyledons of cucumbers and application
  • Gene segments linked with yellow and green cotyledons of cucumbers and application
  • Gene segments linked with yellow and green cotyledons of cucumbers and application

Examples

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Embodiment 2

[0033] Using the obtained gene segment linked to yellow cotyledons of cucumber and the gene segment linked to green cotyledons of cucumber to carry out the method of molecular marker assisted breeding of cucumber, that is to test or identify the purity of cucumber germplasm resources or hybrids.

[0034]The commercial varieties bred by Tianjin Kerun Cucumber Research Institute, Jinyou 3, Jinyou 12, Jinyou 30, Jinyou 32, Jinyou 38 and some cucumber germplasm resources W17-2-1, A13, P53 , P54, P60-1, 66, Q21, etc., using the method of artificial phenotyping at the seedling stage to identify the cotyledon color of the above materials. According to the AFLP-specific primers E-AGA / M-AAG closely linked to the cucumber cotyledon color gene obtained in Example 1, the genomic DNA of the above-mentioned cucumber material is subjected to PCR amplification, and any material with a specific fragment of 258bp in the amplified product is yellow Cotyledon resources; those materials in which o...

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Abstract

The invention discloses gene segments linked with yellow and green cotyledons of cucumbers and application. The gene segment linked with yellow cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.1, and the gene segment linked with green cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.2. The gene segment linked with yellow cotyledons of cucumbers and the gene segment linked with green cotyledons of cucumbers are used for detecting the constructed F2-generation segregation population and cucumber germplasm resources, the identification shows that the coincidence rate of the detection result and the artificial phenotype reaches 96.77%, and the linkage inheritance distance of the genes related to the colors of cucumber cotyledons is 3.2cm. The invention has the advantages of rapidness, accuracy, no influence of environmental conditions and the like, and has a great application value for molecular marker-assisted breeding of cucumbers.

Description

technical field [0001] The invention relates to the technical field of biogenetic engineering, and relates to methods and technologies for conventional hybrid breeding and molecular marker-assisted breeding of cucumbers, in particular to gene fragments linked with the color of cucumber cotyledons and their application. Background technique [0002] Plant leaf color mutation is a relatively common mutation phenomenon in nature, which mainly comes from spontaneous mutation, artificially induced mutation, insertion mutation and gene silencing mutation. Leaf color mutation traits are of great value in genetic breeding as an indicator marker, which can be used to simplify the breeding of improved varieties and the production of hybrids. Mutants with special and excellent traits can also provide excellent germplasm resources for crop genetic breeding . [0003] In 2007 and 2008, the author found several mutation-type individuals in the parental inbred line population of the North...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 王惠哲李淑菊杨瑞环管炜
Owner TIANJIN RES INST OF VEGETABLE
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