Cymbidium sinense culture and propagation method

A technology of mulberry and culture medium, which is applied in the field of plant culture and reproduction, can solve the problems of poor growth and low seedling rate, and achieve the effects of good seedling growth, high seedling rate and low cost.

Inactive Publication Date: 2016-12-07
成都东山兰韵农业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing tissue culture ofte

Method used

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  • Cymbidium sinense culture and propagation method
  • Cymbidium sinense culture and propagation method
  • Cymbidium sinense culture and propagation method

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Embodiment Construction

[0016] The present invention is described in detail below in conjunction with embodiment.

[0017] A method for cultivating orchids provided by the invention comprises the following steps:

[0018] (1) material selection: select the molan that is well-grown without damage by disease and insects that is dug on the same day, adopt pseudobulb as explant, and remove redundant leaves;

[0019] (2) Disinfection of materials: Use soft bristles to preliminarily clean the selected Molan pseudobulbs in a pool to remove surface sediment, then clean them with detergent, rinse them under running water for 60 minutes, and place the Molan pseudobulbs Disinfect with 75% alcohol on the ultra-clean workbench for 30 seconds, rinse with sterile water for 5 times, and then sterilize with 0.2% mercuric chloride solution for 2 to 3 times for a total of 6 to 7 minutes. After being sterilized with mercuric chloride solution for the first time, rinse the pseudobulbs of Molan with sterile water for 5 t...

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Abstract

The invention relates to the technical field of plant cultivation and reproduction, in particular to a method for cultivation and reproduction of orchids, comprising the following steps: material selection, material disinfection, induction cultivation, proliferation cultivation, rooting cultivation, and transplanting; the cultivated seedlings can be made The plant has a high seedling rate, strong adaptability after cultivation, and the seedlings grow well. Through biotechnology means, it meets the needs of commercial production in a short period of time, and provides technical support for the scale and industrialization of enterprises. The cost of the present invention is low, with 1/2MS medium as basic mother solution, and adopts other nutrients to replace expensive coconut juice as nutrient solution, the consumption of its macroelement is half of the macronutrient consumption of standard MS medium, in Under the premise that the average leaf length, the average elongated height of buds and the percentage of large buds are not affected, the preparation cost of the medium is significantly reduced.

Description

technical field [0001] The invention relates to the technical field of plant cultivation and propagation, in particular to a method for cultivating and propagating black orchids. Background technique [0002] Molan, also known as Baosuilan, is a ground plant of the genus Orchid. Pseudobulb ovoid, enclosed in leaf base. Leaves band-shaped, nearly thin leathery, dark green. Scapes emanate from the base of the pseudobulb, erect, thicker, generally slightly longer than the leaves; the color of the flowers varies greatly, usually dark purple or purple-brown with light-colored lips, also yellow-green, pink or white, generally Strong aroma; sepals narrowly oblong or narrowly elliptic; petals nearly narrowly ovate; lips nearly ovate-oblong; stamens slightly curved forward, with narrow wings on both sides; pollen clusters 4 in 2 Yes, broad oval. The capsule is narrowly elliptic, and the flowering period is from October to March of the following year. Growing in forests, shrubs o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈泽彬宋林贵
Owner 成都东山兰韵农业有限公司
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