Method for breeding rice sterile line with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis
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A technology of rice leaf roller and rice stem borer, applied in the field of breeding, can solve the problems of lack of sterile lines resistant to various rice pests, poor reliability of identification results of natural induction in the field, etc.
Inactive Publication Date: 2017-01-04
RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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Problems solved by technology
[0005] In order to solve the current situation of lack of sterile lines resistant to various rice pests due to the fact that the Bt gene-transferred sterile lines cannot be used for production at present, and the problem of poor reliability of the identification results of natural induction in the field, the present invention provides a combination of breeding and breeding. Method for resisting rice stem borer, rice stem borer and rice leaf roller
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Embodiment 1
[0025] 1. In 2010, C22-113 was used as the female parent, and the production and protection material (Rong 18B) with excellent agronomic characteristics and good flowering habit was used as the male parent to obtain hybrid seeds;
[0026] 2. In 2011, planting F 1 generation, remove false hybrids in the field, and harvest F 2 substitute seeds;
[0027] 3. In the spring of 2012, plant F in southern Hainan 2 Generation, sowing April 5-10 in spring, F 2 Rice Lepidoptera pests are not controlled in the Honda period, each F 2 More than 2,000 plants were planted on behalf of the generation, and in F 2 Plant 5 rows of insect-susceptible controls around the generation, use single plants, and select single plants that are also resistant to C. 3 substitute seeds;
[0028] 4. In the autumn of 2012, F was planted in southern Hainan 3 Generation, sow seeds from September 10 to 15 in the autumn of that year, F 3 Rice Lepidoptera pests are not controlled in the generation Honda stage,...
Embodiment 2
[0033] 1.1. In 2010, the breeding material (Rong 18B) with excellent agronomic traits and flowering habit was used as the female parent, and C22-113 was used as the male parent to obtain hybrid seeds;
[0034] 2. In 2011, planting F 1 generation, remove false hybrids in the field, and harvest F 2 substitute seeds;
[0035] 3. In the autumn of 2013, F was planted in southern Hainan 2 Generation, sowing in autumn from September 10 to 15, in F 2 Rice Lepidoptera pests are not controlled in the Honda period, each F 2 More than 2,000 plants were planted on behalf of the generation, and in F 2 Plant 5 rows of insect-susceptible controls around the generation, use single plants, and select single plants that are also resistant to C. 3 substitute seeds;
[0036] 4. In 2014, plant F in southern Hainan 3 Generation, sow seeds from April 5 to 10 in the second spring, F 3 Rice Lepidoptera pests are not controlled in the generation Honda stage, and 30-50 plants are planted for each...
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Abstract
The invention relates to a method for breeding a rice sterile line with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis. The method comprises the following steps: C22-113 containing insect-resistant genes with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis and containing three-line sterile genes is taken as a parent and hybridizes with a three-line maintainer line I with excellent agronomic characters and good flowering habits; a hybrid is taken as a breeding material and subjected to multi-generation self-cross selective breeding with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis as the standard under the condition of high incidence of stem borers, and a strain II with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis is obtained; a three-line sterile line III as a female parent hybridizes with the strain II as a male parent, a sterile plant in the hybrids is subjected to continuous backcross with the male parent strain II as a recurrent parent, the rice sterile line A with resistance to Chilo suppressalis, Scirpophaga incertulas and Cnaphalocrocis medinalis is bred, and the corresponding recurrent parent is the corresponding maintainer line B. The method has no safety dispute, improves a field natural insect luring identification method and improves the accuracy of identification results.
Description
technical field [0001] The invention relates to breeding technology, in particular to a method for breeding rice sterile lines that are also resistant to the rice stem borer, the rice stem borer and the rice leaf roller. Background technique [0002] Chilo suppressalis (Walker)), rice borer (Scirpophaga incertulas (Walk)) and rice leaf roller (Cnaphalocrocismedinalis (Guenée)), which belong to Lepidoptera, are three major insect pests that affect the high and stable yield of rice. The main means of controlling these three pests is to use chemical pesticides. With the continuous increase in the frequency and quantity of pesticide application, on the one hand, it will lead to the increase of pest resistance and fall into a vicious cycle of increasing pesticide dosage. On the other hand, it will cause the accumulation of pesticide pollution in the farmland environment and the increase of pesticide residues in agricultural products, directly threatening the ecology of farmland. ...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 谢戎李永洪王中华刘成元向箭宇
Owner RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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