A kind of protection solution and preservation method for artemia nauplius preservation

A technology of artemia nauplii and protection solution, which is applied in the field of artemia breeding, and can solve the problems of low survival rate and other problems

Active Publication Date: 2020-05-26
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention aims to propose a protective solution and preservation method for the preservation of Artemia nauplii, to solve the problem of low survival rate of Artemia nauplii after cryopreservation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] First use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 22g dissolved salt / L, pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 25°C;

[0023] The hatched Artemia nauplii are added into the protection solution, and the temperature of the protection solution is 25°C.

[0024] The density of the Artemia nauplii in the protection solution is 30 / mL; and the shelled Bohai Bay worm eggs are used.

[0025] After 24 hours of storage at 4°C, the survival rate of Artemia nauplii reached 80%.

[0026] The protected solution includes components with the following concentrations, Angelica polysaccharide 10mg / L; NaCl 20g / L; MgSO 4 0.005g / L; FeCl 3 0.005g / L.

[0027] The polysaccharides of Angelica sinensis are extracted through the follo...

Embodiment 2

[0029] Use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. For incubation, use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 22g dissolved salt / L, pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 25°C;

[0030] The hatched Artemia nauplii were added to the protection solution at a temperature of 25°C.

[0031] The density of the Artemia nauplii in the protection solution is 30 / mL; and the eggs of Bohai Bay are used.

[0032] After 24 hours of storage at 4°C, the survival rate of Artemia nauplii reached 80%.

[0033] The protected solution includes components with the following concentrations, Angelica polysaccharide 9mg / L; NaCl 19g / L; MgSO 4 ...

Embodiment 3

[0036] First use a transparent incubator and a 250ml incubation tube for incubation, and 200ml of incubation solution in the incubation tube for incubation. The hatching solution is prepared by mixing artificial sea crystals with tap water, its salinity is 22g dissolved salt / L, pH is 8.5, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 25°C;

[0037] The hatched Artemia nauplii were added to the protection solution at a temperature of 4°C.

[0038] The density of the Artemia nauplii in the protection solution is 30 / mL; and unshelled Bohai Bay worm eggs are used.

[0039] After 24 hours of storage at 4°C, the survival rate of Artemia nauplii reached 80%.

[0040] The protected solution includes components with the following concentrations, Angelica polysaccharide 11mg / L; NaCl 21g / L; MgSO 4 0.006g / L; FeCl 3 0.006g / L.

[0041] The polysaccharides of Angelica sinensis are extracted through the following steps. Fresh Chinese herbal me...

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PUM

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Abstract

The invention provides a protective solution for preservation of Artemia nauplii and a preservation method. The protective solution comprises 5-15 mg / L of angelica polysaccharides. Density of Artemia nauplii in the protective solution is 1-30 pieces / mL. Preservation temperature is 0-10 DEG C, and pH of the protective solution is 7-10. Stability of survival rate of Artemia nauplii can be guaranteed. Through controlling factors such as temperature, etc., survival rate of Artemia nauplii can be effectively guaranteed at low temperature, survival rate of Artemia nauplii within 24 h reaches 80% and above, and the problem of low survival rate of Artemia nauplii at low temperature is solved.

Description

technical field [0001] The invention relates to the technical field of Artemia culture, in particular to a protection solution and a preservation method for preserving Artemia nauplii. Background technique [0002] Artemia nauplii is generally used as live feed for aquaculture, especially as live feed for marine fish and sea shrimp larvae in the early stage. Generally, there is no such live feed for Artemia nauplii in the market, only Artemia nauplii eggs, nauplii It is hatched from Artemia eggs, and the hatching of Artemia eggs can be completed in the hatching solution, but at present, there are very few researches on the cryopreservation methods of Artemia nauplii. If this problem is solved, Artemia nauplii can be sold directly, reducing the burden on aquaculture enterprises and making Artemia hatching more commercial. Contents of the invention [0003] In view of this, the present invention aims to propose a protection solution and a preservation method for preservatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 于宏孙朦朦路斌王永苓
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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