Preparation method and application of a capillary array for rapid detection of occult blood

A technology of capillary array and capillary, which is applied in the field of biomedical detection, can solve the problems of inability to detect in real time, cannot be stored for a long time, and unstable

Active Publication Date: 2018-06-29
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the instability of the TMB chromogenic matrix itself and the limitations of ready-to-use, this chemical detection method has certain defects, and it cannot be stored for a long time and cannot be detected immediately.

Method used

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  • Preparation method and application of a capillary array for rapid detection of occult blood
  • Preparation method and application of a capillary array for rapid detection of occult blood
  • Preparation method and application of a capillary array for rapid detection of occult blood

Examples

Experimental program
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Embodiment 1

[0054] A method for preparing a capillary array for rapid detection of occult blood, the steps comprising:

[0055] (1) Clean the inner and outer walls of the medical capillary with piranha lotion (a mixed solution of concentrated sulfuric acid and hydrogen peroxide at a volume ratio of 7:3), then wash with ultrapure water and ethanol, dry at 40°C, and then wash with 6.0 wt% Soak in the ethanol solution of hexadecyltrimethylsilane (APTES) for 6 h, take it out, wash it with absolute ethanol, dry it, and set it aside;

[0056] (2) Mix 75.0 mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 25.0 mg N-hydroxysuccinimide (NHS), then add 100 mg Mix p-hydroxyphenylpropionic acid (HPA) evenly, then add the above mixture into 20 mL of MES buffer (50.0 mM), stir at room temperature for 15 min to obtain a mixture containing EDC-NHS-activated HPA, and then add to the above mixture Add 4% (w / v) gelatin (Gel) to the mixture, heat to 50°C under stirring, cool to room t...

Embodiment 2

[0062] A method for preparing a capillary array for rapid detection of occult blood, the steps comprising:

[0063] (1) Clean the inner and outer walls of the medical capillary with piranha lotion (a mixed solution of concentrated sulfuric acid and hydrogen peroxide at a volume ratio of 7:3), then wash with ultrapure water and ethanol, dry at 40°C, and then wash with 6.0 wt% Soak in the ethanol solution of hexadecyltrimethylsilane (APTES) for 6 h, take it out, wash it with absolute ethanol, dry it, and set it aside;

[0064] (2) Mix 75.0 mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 25.0 mg N-hydroxysuccinimide (NHS), then add 20 mg Mix p-hydroxyphenylpropionic acid (HPA) evenly, then add the above mixture into 20 mL of MES buffer (50.0 mM), stir at room temperature for 15 min to obtain a mixture containing EDC-NHS-activated HPA, and then add to the above mixture Add 0.5% (w / v) gelatin (Gel) to the mixture, heat to 50°C under stirring, cool to room ...

Embodiment 3

[0070] A method for preparing a capillary array for rapid detection of occult blood, the steps comprising:

[0071] (1) Clean the inner and outer walls of the medical capillary with piranha lotion (a mixed solution of concentrated sulfuric acid and hydrogen peroxide at a volume ratio of 7:3), then wash with ultrapure water and ethanol, dry at 40°C, and then wash with 6.0 wt% Soak in the ethanol solution of hexadecyltrimethylsilane (APTES) for 6 h, take it out, wash it with absolute ethanol, dry it, and set it aside;

[0072] (2) Mix 75.0 mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 25.0 mg N-hydroxysuccinimide (NHS), then add 120 mg Mix p-hydroxyphenylpropionic acid (HPA) evenly, then add the above mixture into 20 mL of MES buffer (50.0 mM), stir at room temperature for 15 min to obtain a mixture containing EDC-NHS-activated HPA, and then add to the above mixture Add 6% (w / v) gelatin (Gel) to the mixture, heat to 50°C under stirring, cool to room t...

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Abstract

The invention relates to a preparation method and application of a capillary array for rapid occult blood detection. The capillary array realizes rapid, sensitive and high-flux visual detection for occult blood under a capillary phenomenon of automatic suction of a sample according to the development reaction that hemoglobin in the occult blood catalyzes an encapsulation substrate. Firstly, a porous hydrogel-encapsulated peroxidase catalytic reaction substrate is modified on the inner wall of a capillary, thus preparing the visually colorimetric capillary array to automatically suck the sample under the capillary action of the capillary; the capillary can be enabled to display blue colors in different degrees according to the development reaction that the hemoglobin in the occult blood sample catalyzes the porous hydrogel-encapsulated peroxidase catalytic substrate on the inner wall, so that the hemoglobin content of the sample can be directly, quickly, quantitatively or visually determined; and the preparation method has the characteristics of rapidness, sensitivity, high flux, small use amount of the sample, low cost, visualization and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and relates to a method for preparing a capillary array for rapid detection of occult blood. By means of the hemoglobin-specific catalytic substrate color reaction and the function of capillary phenomenon to automatically absorb sample liquid, a fast, sensitive, high-efficiency Through-put Visual Detection of Occult Blood by Capillary Array Analysis. Background technique [0002] Occult blood generally occurs in human saliva, urine, and feces. Usually, red blood cells are usually damaged and cannot be observed with the naked eye or under a microscope. Generally speaking, it can be attributed to the following three reasons, one is inflammation, the other is calculus, and then it is tumor. Therefore, it is very important to develop a simple and accurate analytical method for occult blood detection test for the early diagnosis of many clinical diseases. Historically, many analytical o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72
CPCG01N33/726
Inventor 王桦姜耀张立燕刘凤娟蔡园园
Owner QUFU NORMAL UNIV
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