Oncorhynchus keta walbaum narcosis method

A technology of autumn salmon and anesthetic, which is applied in the field of anesthesia for autumn salmon, achieves the effects of high safety factor, protection of fish welfare, and reduction of injuries

Inactive Publication Date: 2017-04-26
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Factors affecting the anesthetic effect of anesthetic mainly include the type of anesthetic to be anesthetic, concentration of anesthetic, anesthesia water temperature, anesthesia time, time out of w

Method used

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  • Oncorhynchus keta walbaum narcosis method
  • Oncorhynchus keta walbaum narcosis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The anesthesia method of mid-autumn salmon in the present embodiment mainly comprises the following steps:

[0019] 1) Preparation: add the breeding water in the breeding system to the anesthesia box 1 and the recovery box, open the oxygenation equipment in the box, and ensure that the dissolved oxygen content in the water is above 7mg / L; add ice packs to the anesthesia box 1 and the recovery box to ensure The anesthesia water temperature and recovery water temperature are the same as or slightly lower than the culture water temperature. Add the anesthetic MS-222 to the culture water in the anesthesia box 1 and dissolve it completely until the concentration of the anesthetic reaches 0.1 g / L.

[0020] 2), anesthesia: fish 20 autumn salmon seedlings with a body weight range of 40-80g into the anesthesia box 1 in turn and begin to record the anesthesia time. The anesthesia box 1 is covered with a nylon net to prevent the fish from jumping out of the water during the anesth...

Embodiment 2

[0025] Change the concentration of anesthetic MS-222 into 0.2g / L in step 1, implement embodiment 2 in the same manner as embodiment 1.

Embodiment 3

[0027] Change the concentration of anesthetic MS-222 into 0.3g / L in step 1, implement embodiment 3 in the same manner as embodiment 1.

[0028] The results are shown in Table 1.

[0029] Table 1

[0030]

[0031] It can be seen from Table 1 that when the concentration of the anesthetic agent is in the range of 0.1-0.2g / L, with the increase of the concentration of the anesthetic agent, the anesthesia time of autumn salmon is shortened, the recovery time is increased, and there are occasional cases of early awakening during the out-of-water operation. Survival rate is all 100% (embodiment 1, embodiment 2) after resuscitating 24h, and analysis is because anesthesia is not thorough and causes; During the out-of-water operation, no early awakening was found, but the resuscitated autumn salmon died within 24 hours (Example 3), which was analyzed due to excessive anesthesia.

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Abstract

The invention discloses an Oncorhynchus keta walbaum narcosis method. Two narcosis procedures adopting different containers and different anesthetic concentrations are conducted for primary narcosis and deep narcosis of Oncorhynchus keta walbaum, conditions such as the anesthetic concentrations, narcosis time, water-leaving time and the like are optimized, the situations that the Oncorhynchus keta walbaum revives too early due to insufficient narcosis and the Oncorhynchus keta walbaum dies due to excessive narcosis in the operation process can be effectively avoided, harm to the Oncorhynchus keta walbaum is reduced in the water-leaving operation process, and the method has great significance to Oncorhynchus keta walbaum standard breeding operation, breeding data collection, fish welfare guarantee and the like.

Description

technical field [0001] The invention relates to an anesthesia method for fish, in particular to an anesthesia method for autumn salmon. Background technique [0002] Autumn salmon (Oncorhynchus keta Walbaum), also known as chum and salmon, belongs to the order Salmoniformes, the family Salmonidae, and the genus Oncorhynchus. It is a famous rare and precious cold-water fish in my country. During the breeding process, autumn salmon has a ferocious appetite and strong swimming ability, especially at the seedling stage of less than 100g, it is easy to have a strong stress response to various physical and chemical stimuli. Very vulnerable to mechanical damage, leading to bacterial infection. Usually, before leaving the water, the breeding technicians will put the fish into the water containing a certain concentration of a specific anesthetic to anesthetize for a period of time, and when they are under anesthesia, they will quickly take them out of the water for the operation. H...

Claims

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Application Information

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IPC IPC(8): A01K61/13A01K61/10
CPCA01K61/00Y02A40/81
Inventor 姜黎明胡丽萍张黎黎王顺奎刘伟黄绍山刘学貌王潇俊李杰王振洋刘晓
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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