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A method for artificially inducing oviposition of giant clams

A technology of artificial induction and clam, applied in the application, climate change adaptation, fish farming and other directions, can solve problems such as unfavorable long-term development of the clam aquaculture industry, low induction efficiency of clam, and inability to popularize in a large area, etc. Conservation and sustainable development, good promotion, short treatment time

Active Publication Date: 2019-07-12
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in the sun exposure heat stress induction method, the sun exposure is easy to cause damage to giant clams, and the unstable light intensity will lead to low induction efficiency of giant clams. It is reported that the induction rate of this method is about 35%; In the method, although the induction efficiency is significantly improved compared with the first method, the induction rate is about 65%, but the preparation of the giant clam gonad extract requires anatomically mature giant clam, the cost is high, and it cannot be popularized in a large area, which is not conducive to giant clam cultivation Long-term development of the industry

Method used

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  • A method for artificially inducing oviposition of giant clams
  • A method for artificially inducing oviposition of giant clams
  • A method for artificially inducing oviposition of giant clams

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Select 100 sexually mature giant clams over 25cm in size and artificially domesticate them in the breeding pond. The basic water quality requirements are: water temperature 28°C, salinity 33‰, dissolved oxygen 6mg / L, pH value 8.1, transparency 15m;

[0024] (2) Prepare 100 100L white buckets, add 500-mesh screen to filter 30L of seawater in the bucket, and put in a small amount of air stone to inflate;

[0025] (3) Select 40 giant clams with good growth status, use a syringe to inject 2 mL of serotonin solution with a concentration of 20 μmol / L into each gonad, and insert the syringe vertically into the gonad 1 cm from the water outlet to avoid damage to the giant clams due to misoperation and affect oviposition. Put the induced giant clams in 40 white water buckets, the water level is 3cm higher than the giant clams, pay attention to observe the spawning of giant clams;

[0026] (4) After the serotonin solution induction treatment, the giant clams will start to dis...

Embodiment 2

[0028] (1) Select 100 sexually mature giant clams over 25cm in size for artificial domestication in the breeding pond. The basic water quality requirements are: water temperature 29°C, salinity 34‰, dissolved oxygen 7mg / L, pH value 8.2, and transparency 16m;

[0029] (2) Prepare 100 100L white buckets, add 500-mesh screen to filter 30L of seawater in the buckets, and put in a small amount of air stones to inflate;

[0030] (3) Select 40 giant clams with good growth status, use a syringe to inject 2 mL of serotonin solution with a concentration of 20 μmol / L into each gonad, and insert the syringe vertically into the gonads 2 cm from the water outlet to avoid damage to giant clams due to misoperation and affect oviposition. Put the induced giant clams in 40 white water buckets, the water level is 3cm higher than the giant clams, pay attention to observe the spawning of giant clams;

[0031] (4) After the serotonin solution induction treatment, the giant clams will start to disch...

Embodiment 3

[0033] (1) Select 100 sexually mature giant clams over 25cm in size for artificial domestication in the breeding pond. The basic water quality requirements are: water temperature 30°C, salinity 35‰, dissolved oxygen 8mg / L, pH value 8.3, transparency 17m;

[0034] (2) Prepare 100 100L white buckets, add 500-mesh screen to filter 30L of seawater in the bucket, and put in a small amount of air stone to inflate;

[0035] (3) Select 40 giant clams with good growth status, use a syringe to inject 2 mL of serotonin solution with a concentration of 20 μmol / L into each gonad, and insert the syringe vertically into the gonad 1 cm from the water outlet to avoid damage to the giant clams due to misoperation and affect oviposition. Put the induced giant clams in 40 white water buckets, the water level is 3cm higher than the giant clams, pay attention to observe the spawning of giant clams;

[0036] (4) After the serotonin solution induction treatment, the giant clams will start to discharg...

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Abstract

The invention discloses an artificially induced spawning method of tridacnas. The method comprises the following steps that gonad-maturation tridacnas are induced with 5-hydroxytryptamine with the concentration being 20 [mu]mol / L early in the morning under the full moon or the new moon in tridacna mating seasons from July to September every year according to reproductive characteristics of the tridacnas; and the tridacnas are enabled to spawn within 2 h, and specific operation comprises the following sub-steps that the selected gonad-maturation tridacnas are moved out of water, 2 mL of 5-hydroxytryptamine with the concentration being 20 [mu]mol / L is injected into the gonads through an injector, the tridacnas are placed into seawater filtered through a 500-mesh sieve, the seawater level is 2-3 cm higher than the tridacnas, a small amount of oxygen is supplied to the seawater, and expelled sperms and eggs are uniformly blended for fertilization according to the volume ratio of 1:200. The artificially induced spawning method of the tridacnas has the characteristics of easy operation, safety, reliability, high spawning rate, high rate of fertilization, probability of large-scale operation and the like, the status quo of biological resource damage of the tridacnas can be effectively relieved, and a major breakthrough of artificial tridacna breeding is achieved.

Description

technical field [0001] The invention belongs to the field of aquaculture, and relates to a method for breeding medium and large marine shellfish seedlings, in particular to an artificially induced oviposition method for giant clams in the South China Sea. Background technique [0002] Tridacna, commonly known as giant oyster, is a general term for mollusk Phylumbranchia Tridacnaidae. There are 1 family, 2 genera and 9 species. They are widely distributed in tropical seas from the western Pacific to the east coast of Africa in the Indian Ocean. There are 6 species of giant clams that are distributed in my country and are relatively common, mainly distributed in Taiwan, Hainan, Xisha Islands and other South China Sea islands. [0003] Tridacna is the same as other bivalve molluscs, with a mature age of 3 to 5 years. Tridacna is the largest creature among the bivalve molluscs, known as the "king of shellfish". Among them, the largest individual giant clam recorded has a shell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54
CPCY02A40/81
Inventor 周智俞小鹏黄勃唐佳陈国华倪兴振张程凯李灿吴逸波
Owner HAINAN UNIV
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