Antibody regulation double-antigen specific T cell, method for preparing same and application of antibody regulation double-antigen specific T cell

Abstract

The invention provides a double-antigen specific T cell, a method for preparing the same and application of the double-antigen specific T cell. The double-antigen specific T cell comprises mesothelin antigen specific T cell receptor genes and antibody Fc (fibrillar center) fragment specific T cell receptor genes. The antibody Fc fragment specific T cell receptor genes can be mediated by HIF-1 alpha (hypoxia inducible factor-1 alpha) antibodies to specifically recognize HIF-1 alpha antigens. The method includes simultaneously infecting T cells by slow viruses with mesothelin antigen specific T cell receptor genes and slow viruses with antibody Fc fragment specific T cell receptor genes. The double-antigen specific T cell, the method and the application in an embodiment of the invention have the advantages that the double-antigen specific T cell has two specific binding domains and double-specificity for targeting to Meso (mesothelin) and being mediated by the HIF-1 alpha antibodies to specifically recognize the HIF-1 alpha antigens, accordingly, TNBC (triple negative breast cancer) cells can be assuredly specifically recognized and killed by the double-antigen specific T cell, and normal cells of organisms can be prevented from being mistakenly killed.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a double-antigen-specific T cell and a production method thereof, and a pharmaceutical preparation containing double-antigen-specific T cells targeting triple-negative breast cancer cells. Background technique [0002] T lymphocytes are the main effector cells involved in tumor cell immunotherapy, and the activation of T cells is carefully regulated by "dual signals". The first signal is the combination of a specific receptor TCR (T cell receptor) molecule on the surface of T lymphocytes and the antigen peptide presented by the major histocompatibility complex (MHC) on the surface of tumor cells, that is, the T cell’s response to the antigen. Recognition; the second signal is a signal mediated by the interaction of co-stimulatory molecules with corresponding receptors or ligands on the surface of T cells. CD28 / B7 is an important positive co-stimulatory mol...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to overcome the above-mentioned deficiencies of the prior art, and provide a double-antigen-specific T cell and its preparation method to solve the current technology that cannot effectively identify and kill TNBC, and is likely to cause accidental killing of n

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